################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 622 GTAC GT^AC AseI 252 ATTAAT AT^TAAT AsnI 252 ATTAAT AT^TAAT Asp700I 723 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 21 GAGCTC G[AT]GC[AT]^C AvaII 811 GGTCC G^G[AT]CC BanII 21 GAGCTC G[GA]GC[CT]^C BbsI 505 GAAGAC GAAGAC(2/6) BciVI 573 GTATCC GTATCC(6/5) BglII 57 AGATCT A^GATCT BlpI 652 GCTGAGC GC^T[ACGT]AGC BmyI 21 GAGCTC G[AGT]GC[ACT]^C BpmI 478 CTCCAG CTGGAG(16/14) Bpu1102I 652 GCTGAGC GC^T[ACGT]AGC BpuAI 505 GAAGAC GAAGAC(2/6) BsgI 487 GTGCAG GTGCAG(16/14) BsiHKAI 21 GAGCTC G[AT]GC[AT]^C Bsp1286I 21 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 621 TGTACA T^GTACA BspHI 584 TCATGA T^CATGA BsrGI 621 TGTACA T^GTACA BsrI 480 CCAGT ACTGG(1/-1) Cac8I 598 GCTTGC GC[ACGT]^[ACGT]GC CelII 652 GCTGAGC GC^T[ACGT]AGC CfoI 72 GCGC GCG^C EcoRI 415 GAATTC G^AATTC HaeII 71 AGCGCC [GA]GCGC^[CT] HaeIII 499 GGCC GG^CC HhaI 72 GCGC GCG^C HinP1I 72 GCGC G^CGC HphI 154 GGTGA GGTGA(8/7) MaeIII 117 GTTAC ^GT[ACGT]AC NdeI 362 CATATG CA^TATG PflMI 638 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 252 ATTAAT AT^TAAT RcaI 584 TCATGA T^CATGA RsaI 622 GTAC GT^AC SacI 21 GAGCTC GAGCT^C SspBI 621 TGTACA T^GTACA TaqI 731 TCGA T^CGA TthHB8I 731 TCGA T^CGA Van91I 638 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 723 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 244 GATCC GGATC(4/5) 558 GGATC BbvI 328 GCTGC GCAGC(8/12) 331 GCTGC BfaI 353 CTAG C^TAG 833 CTAG BsiYI 521 CCCTTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 638 CCAGGCAATGG BslI 521 CCCTTGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 638 CCAGGCAATGG BsrDI 36 GCAATG GCAATG(2/0) 642 GCAATG BssKI 221 CCAGG ^CC[ACGT]GG 638 CCAGG BstNI 221 CCAGG CC^[AT]GG 638 CCAGG Cfr13I 498 GGGCC G^G[ACGT]CC 811 GGTCC DraI 84 TTTAAA TTT^AAA 702 TTTAAA EcoRII 221 CCAGG ^CC[AT]GG 638 CCAGG Fnu4HI 328 GCTGC GC^[ACGT]GC 331 GCTGC HinfI 212 GATTC G^A[ACGT]TC 396 GAATC ItaI 328 GCTGC GC^[ACGT]GC 331 GCTGC MaeI 353 CTAG C^TAG 833 CTAG MvaI 221 CCAGG CC^[AT]GG 638 CCAGG NlaIV 258 GGCTCC GG[ACGT]^[ACGT]CC 810 GGGTCC Sau96I 498 GGGCC G^G[ACGT]CC 811 GGTCC ScrFI 221 CCAGG CC^[ACGT]GG 638 CCAGG SfcI 549 CTACAG C^T[GA][CT]AG 837 CTACAG SspI 107 AATATT AAT^ATT 123 AATATT TfiI 212 GATTC G^A[AT]TC 396 GAATC TseI 328 GCTGC G^C[AT]GC 331 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AflIII AluI ApoI BspLU11I BstF5I BstYI DdeI DpnII FokI MboI MboII MflI MnlI MseI NdeII NlaIII NspI Sau3AI SfaNI Tru9I Tsp509I TspRI XhoII