Restriction map for
pFN21AB6998
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeIII MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 18 GACGTC GACGT^C
AcyI 18 GACGTC G[GA]^CG[CT]C
AlwI 489 GATCC GGATC(4/5)
AocI 354 CCTCAGG CC^T[ACGT]AGG
AspI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 608 TGCGCA TGC^GCA
BalI 146 TGGCCA TGG^CCA
BanI 73 GGTGCC G^G[CT][GA]CC
BanII 340 GAGCCC G[GA]GC[CT]^C
BbsI 463 GAAGAC GAAGAC(2/6)
BcnI 14 CCCGG CC^[GC]GG
BmyI 340 GAGCCC G[AGT]GC[ACT]^C
BpuAI 463 GAAGAC GAAGAC(2/6)
BsaBI 403 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 18 GACGTC G[GA]^CG[CT]C
BsgI 616 GTGCAG GTGCAG(16/14)
BsmAI 598 GAGAC GTCTC(1/5)
BsmFI 21 GTCCC GGGAC(10/14)
Bsp1286I 340 GAGCCC G[AGT]GC[ACT]^C
BsrBI 624 CCGCTC CCGCTC(-3/-3)
BsrI 481 ACTGG ACTGG(1/-1)
BssHII 134 GCGCGC G^CGCGC
BstF5I 438 CATCC GGATG(2/0)
BstXI 371 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 488 AGATCC [GA]^GATC[CT]
Bsu36I 354 CCTCAGG CC^T[ACGT]AGG
DpnII 489 GATC ^GATC
DraII 422 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 146 TGGCCA [CT]^GGCC[GA]
Eco81I 354 CCTCAGG CC^T[ACGT]AGG
EcoNI 414 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 422 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 438 CATCC GGATG(9/13)
FspI 608 TGCGCA TGC^GCA
HaeIII 147 GGCC GG^CC
HgaI 538 GACGC GACGC(5/10)
Hin1I 18 GACGTC G[GA]^CG[CT]C
HphI 411 TCACC GGTGA(8/7)
MaeII 19 ACGT A^CGT
MamI 403 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 489 GATC ^GATC
MflI 488 AGATCC [GA]^GATC[CT]
MluNI 146 TGGCCA TGG^CCA
MscI 146 TGGCCA TGG^CCA
MslI 529 CACAACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 14 CCCGG CC^[GC]GG
NdeII 489 GATC ^GATC
PflFI 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 422 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 489 GATC ^GATC
SexAI 377 ACCTGGT A^CC[AT]GGT
SfcI 504 CTACAG C^T[GA][CT]AG
Tth111I 589 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 294 TCTAGA T^CTAGA
XhoII 488 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 133 CGCG CG^CG
135 CGCG
AvaII 192 GGACC G^G[AT]CC
423 GGACC
BfaI 295 CTAG C^TAG
427 CTAG
BpmI 5 CTCCAG CTGGAG(16/14)
337 CTGGAG
BsaJI 55 CCCTGG C^C[ACGT][ACGT]GG
392 CCTCGG
BstUI 133 CGCG CG^CG
135 CGCG
Cfr13I 192 GGACC G^G[ACGT]CC
423 GGACC
DdeI 240 CTCAG C^T[ACGT]AG
355 CTCAG
HapII 15 CCGG C^CGG
477 CCGG
HpaII 15 CCGG C^CGG
477 CCGG
MaeI 295 CTAG C^TAG
427 CTAG
MboII 172 GAAGA GAAGA(8/7)
463 GAAGA
MspA1I 142 CAGCTG C[AC]G^C[GT]G
639 CAGCTG
MspI 15 CCGG C^CGG
477 CCGG
MvnI 133 CGCG CG^CG
135 CGCG
PvuII 142 CAGCTG CAG^CTG
639 CAGCTG
Sau96I 192 GGACC G^G[ACGT]CC
423 GGACC
SfaNI 308 GCATC GCATC(5/9)
437 GCATC
SmlI 493 CTCAAG C^T[CT][GA]AG
547 CTCAAG
TaqI 326 TCGA T^CGA
596 TCGA
TspRI 542 CACTG CA[GC]TG[ACGT][ACGT]^
619 CAGTG
TthHB8I 326 TCGA T^CGA
596 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BssKI BstNI Cac8I CfoI EcoRII
Fnu4HI HhaI HinP1I HinfI ItaI
MnlI MvaI MwoI NlaIII NlaIV
PleI ScrFI TseI