Restriction map for
pFN21AA1508
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AclI AflII AscI AseI
AsnI Asp700I AspI AviII BamHI
BciVI BfrI BglII BsaBI BsgI
BsiWI BsmI BspDI BspHI BspLU11I
BsrDI Bst1107I BstAPI BstBI BstZ17I
ClaI DraI DraIII DrdI EcoRI
EcoT22I FseI FspI HincII HindII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI SapI ScaI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 3144 GACGTC GACGT^C
AccIII 1898 TCCGGA T^CCGGA
AcsI 3043 AAATTC [GA]^AATT[CT]
AflIII 2267 ACGTGT A^C[GA][CT]GT
AgeI 899 ACCGGT A^CCGGT
AhdI 3349 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 154 GTGCAC G^TGCAC
ApaLI 154 GTGCAC G^TGCAC
ApoI 3043 AAATTC [GA]^AATT[CT]
AspEI 3349 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 40 CCTAGG C^CTAGG
BfaI 41 CTAG C^TAG
BlnI 40 CCTAGG C^CTAGG
BlpI 2130 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2130 GCTGAGC GC^T[ACGT]AGC
BseAI 1898 TCCGGA T^CCGGA
BsmBI 852 GAGACG CGTCTC(1/5)
Bsp1407I 83 TGTACA T^GTACA
BspEI 1898 TCCGGA T^CCGGA
BsrGI 83 TGTACA T^GTACA
BssHII 2116 GCGCGC G^CGCGC
BstEII 3171 GGTGACC G^GT[ACGT]ACC
BstPI 3171 GGTGACC G^GT[ACGT]ACC
BstYI 3012 GGATCT [GA]^GATC[CT]
CelII 2130 GCTGAGC GC^T[ACGT]AGC
CpoI 1231 CGGACCG CG^G[AT]CCG
Eam1105I 3349 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3171 GGTGACC G^GT[ACGT]ACC
EcoRV 2008 GATATC GAT^ATC
HindIII 2419 AAGCTT A^AGCTT
KspI 2820 CCGCGG CCGC^GG
MaeI 41 CTAG C^TAG
MflI 3012 GGATCT [GA]^GATC[CT]
MroI 1898 TCCGGA T^CCGGA
PinAI 899 ACCGGT A^CCGGT
RsrII 1231 CGGACCG CG^G[AT]CCG
SacI 989 GAGCTC GAGCT^C
SacII 2820 CCGCGG CCGC^GG
SexAI 827 ACCAGGT A^CC[AT]GGT
SphI 2719 GCATGC GCATG^C
SspBI 83 TGTACA T^GTACA
TaqI 2951 TCGA T^CGA
Tsp509I 3044 AATT ^AATT
TthHB8I 2951 TCGA T^CGA
XhoII 3012 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 212 GGTACC G^GTACC
831 GGTACC
Aor51HI 1802 AGCGCT AGC^GCT
2852 AGCGCT
Asp718I 212 GGTACC G^GTACC
831 GGTACC
BalI 1553 TGGCCA TGG^CCA
1787 TGGCCA
BbrPI 2266 CACGTG CAC^GTG
2534 CACGTG
BbvCI 581 CCTCAGC CCTCAGC(-5/-2)
2314 GCTGAGG
BcgI 68 CGAGGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
984 GCACCGAGCTCG
BclI 2408 TGATCA T^GATCA
2873 TGATCA
BsaAI 2266 CACGTG [CT]AC^GT[GA]
2534 CACGTG
BssSI 2741 CACGAG CACGAG(-5/-1)
2230 CTCGTG
BstXI 782 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2445 CCATAGCTCTGG
Eco47III 1802 AGCGCT AGC^GCT
2852 AGCGCT
EcoNI 165 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
548 CCTCCACCAGG
FbaI 2408 TGATCA T^GATCA
2873 TGATCA
KpnI 212 GGTACC GGTAC^C
831 GGTACC
MluNI 1553 TGGCCA TGG^CCA
1787 TGGCCA
MscI 1553 TGGCCA TGG^CCA
1787 TGGCCA
MseI 2423 TTAA T^TAA
2707 TTAA
MslI 561 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3025 CACTACTATG
NaeI 2157 GCCGGC GCC^GGC
2182 GCCGGC
NcoI 227 CCATGG C^CATGG
866 CCATGG
NgoMIV 2157 GCCGGC G^CCGGC
2182 GCCGGC
PflMI 22 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
757 CCAGAGCCTGG
PmaCI 2266 CACGTG CAC^GTG
2534 CACGTG
PmlI 2266 CACGTG CAC^GTG
2534 CACGTG
SbfI 1167 CCTGCAGG CCTGCA^GG
2841 CCTGCAGG
Sse8387I 1167 CCTGCAGG CCTGCA^GG
2841 CCTGCAGG
StuI 803 AGGCCT AGG^CCT
1328 AGGCCT
TfiI 2043 GATTC G^A[AT]TC
2467 GATTC
Tru9I 2423 TTAA T^TAA
2707 TTAA
Van91I 22 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
757 CCAGAGCCTGG
XcmI 96 CCAGGACGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
554 CCAGGGCCACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI StyI TseI Tsp45I
TspRI XmaCI XmaI