Restriction map for
pFN21AA1508
(Circular) (Six-base) MAPSORT of: pFN21AA1508.seq Check: 1695 from: 1 to: 8083
pFN21AA1508 8083 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2659 4184 2659
Size: 1525 6558
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5153 5893 278
Size: 53 83 186 936 3617 740 2468
Fragments arranged by size:
3617 2468 936 740 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5426 1937
Size: 3489 4594
Acc65I G'GTAC_C
Cuts at: 2217 2836 5404 2217
Size: 619 2568 4896
Fragments arranged by size:
4896 2568 619
AclI AA'CG_TT
Cuts at: 1563 6210 6583 1563
Size: 4647 373 3063
Fragments arranged by size:
4647 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 3969 3991 5224 6146 7530 7798 1632
Size: 560 1777 22 1233 922 1384 268 1917
Fragments arranged by size:
1917 1777 1384 1233 922 560 268 22
AfeI AGC'GCT
Cuts at: 3809 4859 3809
Size: 1050 7033
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7886
AflIII A'CryG_T
Cuts at: 1203 4272 6982 7959 1203
Size: 3069 2710 977 1327
Fragments arranged by size:
3069 2710 1327 977
AgeI A'CCGG_T
Cuts at: 2904 6914 2904
Size: 4010 4073
AhdI GACnn_n'nnGTC
Cuts at: 5359 6812 5359
Size: 1453 6630
AleI CACnn'nnGTG
Cuts at: 1166 2570 1166
Size: 1404 6679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2795 3660 4100 5357 1184
Size: 1611 865 440 1257 3910
Fragments arranged by size:
3910 1611 1257 865 440
AlwNI CAG_nnn'CTG
Cuts at: 2768 3181 3683 4561 5826 7398 2768
Size: 413 502 878 1265 1572 3453
Fragments arranged by size:
3453 1572 1265 878 502 413
ApaI G_GGCC'C
Cuts at: 2027 2887 3150 3382 4464 2027
Size: 860 263 232 1082 5646
Fragments arranged by size:
5646 1082 860 263 232
ApaLI G'TGCA_C
Cuts at: 2159 6140 7296 2159
Size: 3981 1156 2946
Fragments arranged by size:
3981 2946 1156
ApoI r'AATT_y
Cuts at: 1493 5048 5392 5618 5770 7879 1493
Size: 3555 344 226 152 2109 1697
Fragments arranged by size:
3555 2109 1697 344 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6637 160
Size: 872 5605 1606
Fragments arranged by size:
5605 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8083
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2547 2632 2880 3132 3191 3375
Size: 73 204 400 85 248 252 59 184
Cuts at: 3375 4157 4916 5356 5408 1870
Size: 782 759 440 52 4545
Fragments arranged by size:
4545 782 759 440 400 252 248 204
184 85 73 59 52
AvrII C'CTAG_G
Cuts at: 2045 2045
Size: 8083
BamHI G'GATC_C
Cuts at: 5413 5780 5413
Size: 367 7716
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2217 2572 2714 2779
Size: 325 217 627 262 168 355 142 65
Cuts at: 2779 2836 2862 2988 3692 3867 3941 4210 5404
Size: 57 26 126 704 175 74 269 1194
Cuts at: 5404 6859 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1194 704 627 355 325 269
262 217 175 168 142 126 74 65
57 26
BanII G_rGCy'C
Cuts at: 1391 2027 2232 2583 2640 2742 2887 2998 3150
Size: 636 205 351 57 102 145 111 152
Cuts at: 3150 3382 3606 4158 4464 5402 1391
Size: 232 224 552 306 938 4072
Fragments arranged by size:
4072 938 636 552 351 306 232 224
205 152 145 111 102 57
BbeI G_GCGC'C
Cuts at: 2053 3871 4214 2053
Size: 1818 343 5922
Fragments arranged by size:
5922 1818 343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2271 2348 3576 928
Size: 971 372 77 1228 5435
Fragments arranged by size:
5435 1228 971 372 77
BbvCI CC'TCA_GC
Cuts at: 2587 4320 2587
Size: 1733 6350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2062 2096 2978 3012 6274 6308 1903
Size: 34 125 34 882 34 3262 34 3678
Fragments arranged by size:
3678 3262 882 125 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5975 7185 5975
Size: 1210 6873
BclI T'GATC_A
Cuts at: 1548 4413 4878 1548
Size: 2865 465 4753
Fragments arranged by size:
4753 2865 465
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 2882 2973
Size: 107 122 71 631 605 610 600 91
Cuts at: 2973 2982 3177 3240 3557 4503 6694 136
Size: 9 195 63 317 946 2191 1525
Fragments arranged by size:
2191 1525 946 631 610 605 600 317
195 122 107 91 71 63 9
BglII A'GATC_T
Cuts at: 8078 8078
Size: 8083
BlpI GC'TnA_GC
Cuts at: 4136 5500 4136
Size: 1364 6719
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2163 2665 2784 2867 2887 3058 3150
Size: 237 136 502 119 83 20 171 92
Cuts at: 3150 3382 3695 3944 4464 4782 5304 5346 6144
Size: 232 313 249 520 318 522 42 798
Cuts at: 6144 7300 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 798 522 520 502 318 313
249 237 232 171 136 119 92 83
42 20
BmgBI CAC'GTC
Cuts at: 1318 3640 1318
Size: 2322 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 2592 3053
Size: 416 53 83 399 24 613 562 461
Cuts at: 3053 3301 5814 6772 442
Size: 248 2513 958 1753
Fragments arranged by size:
2513 1753 958 613 562 461 416 399
248 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8083
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 8083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2411 2499 2644 2816 3404 3646 4691
Size: 1007 165 88 145 172 588 242 1045
Cuts at: 4691 4775 6743 1239
Size: 84 1968 2579
Fragments arranged by size:
2579 1968 1045 1007 588 242 172 165
145 88 84
Bpu10I CC'TnA_GC
Cuts at: 2577 2587 4320 2577
Size: 10 1733 6340
Fragments arranged by size:
6340 1733 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3034 4493 4508 4877 5051 6208 7073 7371 3034
Size: 1459 15 369 174 1157 865 298 3746
Fragments arranged by size:
3746 1459 1157 865 369 298 174 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2295 3664 4250 6746 882
Size: 617 796 1369 586 2496 2219
Fragments arranged by size:
2496 2219 1369 796 617 586
BsaAI yAC'GTr
Cuts at: 493 1204 4273 4541 5874 493
Size: 711 3069 268 1333 2702
Fragments arranged by size:
3069 2702 1333 711 268
BsaBI GATnn'nnATC
Cuts at: 8077 8077
Size: 8083
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 2747 3733 3868
Size: 53 83 186 936 517 697 986 135
Cuts at: 3868 4211 5150 5890 6272 6907 275
Size: 343 939 740 382 635 1451
Fragments arranged by size:
1451 986 939 936 740 697 635 517
382 343 186 135 83 53
BsaWI w'CCGG_w
Cuts at: 2904 3207 3903 6516 6914 7188 7335 7746 2904
Size: 303 696 2613 398 274 147 411 3241
Fragments arranged by size:
3241 2613 696 411 398 303 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2846 2876 1178
Size: 30 165 30 1443 30 6385
Fragments arranged by size:
6385 1443 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 2722 2927 3236 3769 4279 4688 1186
Size: 1028 508 205 309 533 510 409 4581
Fragments arranged by size:
4581 1028 533 510 508 409 309 205
BseYI C'CCAG_C
Cuts at: 2705 2786 2866 2971 3087 3096 3368 3614 3887
Size: 81 80 105 116 9 272 246 273
Cuts at: 3887 3936 4339 7286 7782 2705
Size: 49 403 2947 496 3006
Fragments arranged by size:
3006 2947 496 403 273 272 246 116
105 81 80 49 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 3362 4188 5533 6294 7322
Size: 275 186 156 1205 826 1345 761 1028
Cuts at: 7322 1540
Size: 2301
Fragments arranged by size:
2301 1345 1205 1028 826 761 275 186
156
BsiHKAI G_wGCw'C
Cuts at: 2042 2163 2998 6144 6229 7300 2042
Size: 121 835 3146 85 1071 2825
Fragments arranged by size:
3146 2825 1071 835 121 85
BsmI GAATG_Cn'
Cuts at: 5594 5687 5594
Size: 93 7990
BsmBI CGTCTCn'nnnn_
Cuts at: 2851 2851
Size: 8083
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2042 2163 2232 2583 2640 2665
Size: 399 237 15 121 69 351 57 25
Cuts at: 2665 2742 2784 2867 2887 2998 3058 3150 3382
Size: 77 42 83 20 111 60 92 232
Cuts at: 3382 3606 3695 3944 4158 4464 4782 5304 5346
Size: 224 89 249 214 306 318 522 42
Cuts at: 5346 5402 6144 6229 7300 1391
Size: 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 399 351 318 306
249 237 232 224 214 121 111 92
89 85 83 77 69 60 57 56
42 42 25 20 15
BspEI T'CCGG_A
Cuts at: 3903 7746 3903
Size: 3843 4240
BspHI T'CATG_A
Cuts at: 5972 7702 5972
Size: 1730 6353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2659 3147 3795 4184 4569 4854 5438 844
Size: 1815 488 648 389 385 285 584 3489
Fragments arranged by size:
3489 1815 648 584 488 389 385 285
BsrBI CCG'CTC
Cuts at: 1394 1823 3079 3777 4147 4646 5970 1394
Size: 429 1256 698 370 499 1324 3507
Fragments arranged by size:
3507 1324 1256 698 499 429 370
BsrDI GCAATG_nn'
Cuts at: 65 1248 6578 6752 65
Size: 1183 5330 174 1396
Fragments arranged by size:
5330 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2304 2904 3239 4162 4187 6727 6914
Size: 110 494 600 335 923 25 2540 187
Cuts at: 6914 1700
Size: 2869
Fragments arranged by size:
2869 2540 923 600 494 335 187 110
25
BsrGI T'GTAC_A
Cuts at: 96 2088 96
Size: 1992 6091
BssHII G'CGCG_C
Cuts at: 1925 4121 1925
Size: 2196 5887
BssSI C'ACGA_G
Cuts at: 4235 4746 6143 7155 4235
Size: 511 1397 1012 5163
Fragments arranged by size:
5163 1397 1012 511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8083
BstBI TT'CG_AA
Cuts at: 6887 6887
Size: 8083
BstEII G'GTnAC_C
Cuts at: 1184 5176 1184
Size: 3992 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 2794 4457 2794
Size: 1663 6420
BstYI r'GATC_y
Cuts at: 1972 5017 5413 5780 6165 6182 7634 7720 7732
Size: 3045 396 367 385 17 1452 86 12
Cuts at: 7732 8078 1972
Size: 346 1977
Fragments arranged by size:
3045 1977 1452 396 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3422 4547 5292 3422
Size: 1125 745 6213
Fragments arranged by size:
6213 1125 745
BtgI C'CryG_G
Cuts at: 513 1065 2004 2194 2232 2871 2933 3801 4689
Size: 552 939 190 38 639 62 868 888
Cuts at: 4689 4825 513
Size: 136 3771
Fragments arranged by size:
3771 939 888 868 639 552 190 136
62 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3423 3837 5059 487
Size: 683 56 2197 414 1222 3511
Fragments arranged by size:
3511 2197 1222 683 414 56
BtsI GCAGTG_nn'
Cuts at: 2161 2591 4800 5304 5599 6393 6413 2161
Size: 430 2209 504 295 794 20 3831
Fragments arranged by size:
3831 2209 794 504 430 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8083
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8048
DraI TTT'AAA
Cuts at: 5387 5387
Size: 8083
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7090 809
Size: 507 5774 1802
Fragments arranged by size:
5774 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 3359 3558
Size: 54 1425 50 275 18 324 1205 199
Cuts at: 3558 3769 3792 4185 5530 6419 8048 8
Size: 211 23 393 1345 889 1629 43
Fragments arranged by size:
1629 1425 1345 1205 889 393 324 275
211 199 54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2154 3359 4185 5530 1537
Size: 275 342 1205 826 1345 4090
Fragments arranged by size:
4090 1345 1205 826 342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 2683 5244 6013 1353
Size: 822 508 2561 769 3423
Fragments arranged by size:
3423 2561 822 769 508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6670 7044 7190 387
Size: 1064 138 5081 374 146 1280
Fragments arranged by size:
5081 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2996 2996
Size: 8083
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2246 2411 2499 2644 2816 3404
Size: 393 560 54 165 88 145 172 588
Cuts at: 3404 3646 3969 3991 4691 4775 5224 6146 6743
Size: 242 323 22 700 84 449 922 597
Cuts at: 6743 7530 7798 1239
Size: 787 268 1524
Fragments arranged by size:
1524 922 787 700 597 588 560 449
393 323 268 242 172 165 145 88
84 54 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2557 2174
Size: 383 7700
EcoO109I rG'GnC_Cy
Cuts at: 2204 3136 3146 3147 3154 3178 3379 3924 4387
Size: 932 10 1 7 24 201 545 463
Cuts at: 4387 4504 4558 2204
Size: 117 54 5729
Fragments arranged by size:
5729 932 545 463 201 117 54 24
10 7 1
EcoRI G'AATT_C
Cuts at: 5392 5770 5392
Size: 378 7705
EcoRV GAT'ATC
Cuts at: 4015 4015
Size: 8083
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4918 4918
Size: 8083
FspI TGC'GCA
Cuts at: 6589 6589
Size: 8083
HaeII r_GCGC'y
Cuts at: 1678 2053 2895 3235 3811 3871 4214 4861 7230
Size: 375 842 340 576 60 343 647 2369
Cuts at: 7230 1678
Size: 2531
Fragments arranged by size:
2531 2369 842 647 576 375 343 340
60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2335 2367 5002
Size: 32 135 32 452 32 279 32 2635
Cuts at: 5002 5034 5345 5377 6724 6756 6798 6830 1373
Size: 32 311 32 1347 32 42 32 2626
Fragments arranged by size:
2635 2626 1347 452 311 279 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5427 5673 669
Size: 988 281 3489 246 3079
Fragments arranged by size:
3489 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4424 748
Size: 3676 4407
HpaI GTT'AAC
Cuts at: 5673 5673
Size: 8083
KasI G'GCGC_C
Cuts at: 2049 3867 4210 2049
Size: 1818 343 5922
Fragments arranged by size:
5922 1818 343
KpnI G_GTAC'C
Cuts at: 2221 2840 5408 2221
Size: 619 2568 4896
Fragments arranged by size:
4896 2568 619
MfeI C'AATT_G
Cuts at: 5682 5682
Size: 8083
MluI A'CGCG_T
Cuts at: 7959 7959
Size: 8083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 5355 7197 7381 1305
Size: 1584 2466 1842 184 2007
Fragments arranged by size:
2466 2007 1842 1584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3560 3794 10
Size: 54 1425 343 1728 234 4299
Fragments arranged by size:
4299 1728 1425 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 5034 6041 6400 6559 518
Size: 648 1404 2464 1007 359 159 2042
Fragments arranged by size:
2464 2042 1404 1007 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2431 2971 3457 4080 4292 4827 5366
Size: 408 417 540 486 623 212 535 539
Cuts at: 5366 5499 6176 7324 7569 1606
Size: 133 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 623 540 539 535 486
417 408 245 212 133
NaeI GCC'GGC
Cuts at: 4164 4189 4164
Size: 25 8058
NarI GG'CG_CC
Cuts at: 2050 3868 4211 2050
Size: 1818 343 5922
Fragments arranged by size:
5922 1818 343
NcoI C'CATG_G
Cuts at: 513 1065 2004 2232 2871 513
Size: 552 939 228 639 5725
Fragments arranged by size:
5725 939 639 552 228
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8083
NgoMIV G'CCGG_C
Cuts at: 4162 4187 4162
Size: 25 8058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8083
NotI GC'GGCC_GC
Cuts at: 5530 5530
Size: 8083
NspI r_CATG'y
Cuts at: 1132 1229 4339 4728 5188 5441 6986 1132
Size: 97 3110 389 460 253 1545 2229
Fragments arranged by size:
3110 2229 1545 460 389 253 97
PasI CC'CwG_GG
Cuts at: 1348 3348 4608 1348
Size: 2000 1260 4823
Fragments arranged by size:
4823 2000 1260
PciI A'CATG_T
Cuts at: 6982 6982
Size: 8083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2033 2768 1108
Size: 925 735 6423
Fragments arranged by size:
6423 925 735
PfoI T'CCnGG_A
Cuts at: 3264 4359 5013 3264
Size: 1095 654 6334
Fragments arranged by size:
6334 1095 654
PmeI GTTT'AAAC
Cuts at: 5387 5387
Size: 8083
PmlI CAC'GTG
Cuts at: 4273 4541 4273
Size: 268 7815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6152 6184 7665 7697 6152
Size: 32 1481 32 6538
Fragments arranged by size:
6538 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2204 3136 3924 4387 4558 2204
Size: 932 788 463 171 5729
Fragments arranged by size:
5729 932 788 463 171
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8083
PsiI TTA'TAA
Cuts at: 4041 5653 4041
Size: 1612 6471
PspOMI G'GGCC_C
Cuts at: 2023 2883 3146 3378 4460 2023
Size: 860 263 232 1082 5646
Fragments arranged by size:
5646 1082 860 263 232
PstI C_TGCA'G
Cuts at: 830 2168 3177 3755 4594 4851 5435 830
Size: 1338 1009 578 839 257 584 3478
Fragments arranged by size:
3478 1338 1009 839 584 578 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8083
PvuII CAG'CTG
Cuts at: 2014 2431 2971 3457 2014
Size: 417 540 486 6640
Fragments arranged by size:
6640 540 486 417
RsrII CG'GwC_CG
Cuts at: 3237 3237
Size: 8083
SacI G_AGCT'C
Cuts at: 2998 2998
Size: 8083
SacII CC_GC'GG
Cuts at: 4828 4828
Size: 8083
SalI G'TCGA_C
Cuts at: 1936 5425 1936
Size: 3489 4594
SbfI CC_TGCA'GG
Cuts at: 3177 4851 5435 3177
Size: 1674 584 5825
Fragments arranged by size:
5825 1674 584
ScaI AGT'ACT
Cuts at: 6331 8043 6331
Size: 1712 6371
SexAI A'CCwGG_T
Cuts at: 2832 2832
Size: 8083
SfcI C'TryA_G
Cuts at: 826 1045 2164 3173 3214 3751 4249 4590 4808
Size: 219 1119 1009 41 537 498 341 218
Cuts at: 4808 4847 5431 6566 7247 7438 826
Size: 39 584 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 1009 681 584 537 498
341 219 218 191 41 39
SfiI GGCCn_nnn'nGGCC
Cuts at: 2882 3177 3557 4503 2882
Size: 295 380 946 6462
Fragments arranged by size:
6462 946 380 295
SfoI GGC'GCC
Cuts at: 2051 3869 4212 2051
Size: 1818 343 5922
Fragments arranged by size:
5922 1818 343
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8083
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8083
SmaI CCC'GGG
Cuts at: 1872 2549 2634 2882 3377 4159 5358 5410 1872
Size: 677 85 248 495 782 1199 52 4545
Fragments arranged by size:
4545 1199 782 677 495 248 85 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3013 4508 4523 4892 5066 6187
Size: 197 926 1070 1495 15 369 174 1121
Cuts at: 6187 7088 7350 820
Size: 901 262 1553
Fragments arranged by size:
1553 1495 1121 1070 926 901 369 262
197 174 15
SnaBI TAC'GTA
Cuts at: 493 5874 493
Size: 5381 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8083
SphI G_CATG'C
Cuts at: 1132 4728 5441 1132
Size: 3596 713 3774
Fragments arranged by size:
3774 3596 713
SrfI GCCC'GGGC
Cuts at: 2549 4159 2549
Size: 1610 6473
SspI AAT'ATT
Cuts at: 5 52 6007 5
Size: 47 5955 2081
Fragments arranged by size:
5955 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 3335 1517
Size: 1293 525 6265
Fragments arranged by size:
6265 1293 525
StyI C'CwwG_G
Cuts at: 513 1065 2004 2045 2232 2409 2682 2871 3481
Size: 552 939 41 187 177 273 189 610
Cuts at: 3481 3927 5522 513
Size: 446 1595 3074
Fragments arranged by size:
3074 1595 939 610 552 446 273 189
187 177 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2025 3361 6110 6127 6280 6465 6910
Size: 1255 445 1336 2749 17 153 185 445
Cuts at: 6910 325
Size: 1498
Fragments arranged by size:
2749 1498 1336 1255 445 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 6329 8041
Size: 274 80 33 51 1443 111 4241 1712
Cuts at: 8041 96
Size: 138
Fragments arranged by size:
4241 1712 1443 274 138 111 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2737 2769 2846 2878 8043 8075 2737
Size: 32 77 32 5165 32 2745
Fragments arranged by size:
5165 2745 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8083
XbaI T'CTAG_A
Cuts at: 5419 5419
Size: 8083
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2566 2108
Size: 458 7625
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8083
XmaI C'CCGG_G
Cuts at: 1870 2547 2632 2880 3375 4157 5356 5408 1870
Size: 677 85 248 495 782 1199 52 4545
Fragments arranged by size:
4545 1199 782 677 495 248 85 52
XmnI GAAnn'nnTTC
Cuts at: 6212 6212
Size: 8083
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5151 5891 276
Size: 53 83 186 936 3617 740 2468
Fragments arranged by size:
3617 2468 936 740 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII FseI
FspAI NruI NsiI PacI PspXI PsrI SanDI SapI
SwaI