Restriction map for
pFN21AA1508
(Linear) (Six-base) MAPSORT of: KIAA1508.seq Check: 8064 from: 1 to: 3378
KIAA1508.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 654 2179 3378
Size: 654 1525 1199
Fragments arranged by size:
1525 1199 654
AatII G_ACGT'C
Cuts at: 0 3148 3378
Size: 3148 230
Acc65I G'GTAC_C
Cuts at: 0 212 831 3378
Size: 212 619 2547
Fragments arranged by size:
2547 619 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1964 1986 3219 3378
Size: 187 1777 22 1233 159
Fragments arranged by size:
1777 1233 187 159 22
AfeI AGC'GCT
Cuts at: 0 1804 2854 3378
Size: 1804 1050 524
Fragments arranged by size:
1804 1050 524
AflIII A'CryG_T
Cuts at: 0 2267 3378
Size: 2267 1111
AgeI A'CCGG_T
Cuts at: 0 899 3378
Size: 899 2479
AhdI GACnn_n'nnGTC
Cuts at: 0 3354 3378
Size: 3354 24
AleI CACnn'nnGTG
Cuts at: 0 565 3378
Size: 565 2813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 790 1655 2095 3352 3378
Size: 790 865 440 1257 26
Fragments arranged by size:
1257 865 790 440 26
AlwNI CAG_nnn'CTG
Cuts at: 0 763 1176 1678 2556 3378
Size: 763 413 502 878 822
Fragments arranged by size:
878 822 763 502 413
ApaI G_GGCC'C
Cuts at: 0 22 882 1145 1377 2459 3378
Size: 22 860 263 232 1082 919
Fragments arranged by size:
1082 919 860 263 232 22
ApaLI G'TGCA_C
Cuts at: 0 154 3378
Size: 154 3224
ApoI r'AATT_y
Cuts at: 0 3043 3378
Size: 3043 335
AvaI C'yCGr_G
Cuts at: 0 142 542 627 875 1127 1186 1370 2152
Size: 142 400 85 248 252 59 184 782
Cuts at: 2152 2911 3351 3378
Size: 759 440 27
Fragments arranged by size:
782 759 440 400 252 248 184 142
85 59 27
AvrII C'CTAG_G
Cuts at: 0 40 3378
Size: 40 3338
BanI G'GyrC_C
Cuts at: 0 44 212 567 709 774 831 857 983
Size: 44 168 355 142 65 57 26 126
Cuts at: 983 1687 1862 1936 2205 3378
Size: 704 175 74 269 1173
Fragments arranged by size:
1173 704 355 269 175 168 142 126
74 65 57 44 26
BanII G_rGCy'C
Cuts at: 0 22 227 578 635 737 882 993 1145
Size: 22 205 351 57 102 145 111 152
Cuts at: 1145 1377 1601 2153 2459 3378
Size: 232 224 552 306 919
Fragments arranged by size:
919 552 351 306 232 224 205 152
145 111 102 57 22
BbeI G_GCGC'C
Cuts at: 0 48 1866 2209 3378
Size: 48 1818 343 1169
Fragments arranged by size:
1818 1169 343 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 266 343 1571 3378
Size: 266 77 1228 1807
Fragments arranged by size:
1807 1228 266 77
BbvCI CC'TCA_GC
Cuts at: 0 582 2315 3378
Size: 582 1733 1063
Fragments arranged by size:
1733 1063 582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 57 91 973 1007 3378
Size: 57 34 882 34 2371
Fragments arranged by size:
2371 882 57 34 34
BclI T'GATC_A
Cuts at: 0 2408 2873 3378
Size: 2408 465 505
Fragments arranged by size:
2408 505 465
BglI GCCn_nnn'nGGC
Cuts at: 0 277 877 968 977 1172 1235 1552 2498
Size: 277 600 91 9 195 63 317 946
Cuts at: 2498 3378
Size: 880
Fragments arranged by size:
946 880 600 317 277 195 91 63
9
BlpI GC'TnA_GC
Cuts at: 0 2131 3378
Size: 2131 1247
Bme1580I G_kGCm'C
Cuts at: 0 22 158 660 779 862 882 1053 1145
Size: 22 136 502 119 83 20 171 92
Cuts at: 1145 1377 1690 1939 2459 2777 3299 3341 3378
Size: 232 313 249 520 318 522 42 37
Fragments arranged by size:
522 520 502 318 313 249 232 171
136 119 92 83 42 37 22 20
BmgBI CAC'GTC
Cuts at: 0 1635 3378
Size: 1635 1743
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 587 1048 1296 3378
Size: 25 562 461 248 2082
Fragments arranged by size:
2082 562 461 248 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 3378
Size: 597 2781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 406 494 639 811 1399 1641 2686
Size: 241 165 88 145 172 588 242 1045
Cuts at: 2686 2770 3378
Size: 84 608
Fragments arranged by size:
1045 608 588 242 241 172 165 145
88 84
Bpu10I CC'TnA_GC
Cuts at: 0 572 582 2315 3378
Size: 572 10 1733 1063
Fragments arranged by size:
1733 1063 572 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 2488 2503 2872 3046 3378
Size: 1029 1459 15 369 174 332
Fragments arranged by size:
1459 1029 369 332 174 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 290 1659 2245 3378
Size: 290 1369 586 1133
Fragments arranged by size:
1369 1133 586 290
BsaAI yAC'GTr
Cuts at: 0 2268 2536 3378
Size: 2268 268 842
Fragments arranged by size:
2268 842 268
BsaHI Gr'CG_yC
Cuts at: 0 45 742 1728 1863 2206 3145 3378
Size: 45 697 986 135 343 939 233
Fragments arranged by size:
986 939 697 343 233 135 45
BsaWI w'CCGG_w
Cuts at: 0 899 1202 1898 3378
Size: 899 303 696 1480
Fragments arranged by size:
1480 899 696 303
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 841 871 3378
Size: 841 30 2507
Fragments arranged by size:
2507 841 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 209 717 922 1231 1764 2274 2683 3378
Size: 209 508 205 309 533 510 409 695
Fragments arranged by size:
695 533 510 508 409 309 209 205
BseYI C'CCAG_C
Cuts at: 0 700 781 861 966 1082 1091 1363 1609
Size: 700 81 80 105 116 9 272 246
Cuts at: 1609 1882 1931 2334 3378
Size: 273 49 403 1044
Fragments arranged by size:
1044 700 403 273 272 246 116 105
81 80 49 9
BsiEI CG_ry'CG
Cuts at: 0 152 1357 2183 3378
Size: 152 1205 826 1195
Fragments arranged by size:
1205 1195 826 152
BsiHKAI G_wGCw'C
Cuts at: 0 37 158 993 3378
Size: 37 121 835 2385
Fragments arranged by size:
2385 835 121 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 846 3378
Size: 846 2532
Bsp1286I G_dGCh'C
Cuts at: 0 22 37 158 227 578 635 660 737
Size: 22 15 121 69 351 57 25 77
Cuts at: 737 779 862 882 993 1053 1145 1377 1601
Size: 42 83 20 111 60 92 232 224
Cuts at: 1601 1690 1939 2153 2459 2777 3299 3341 3378
Size: 89 249 214 306 318 522 42 37
Fragments arranged by size:
522 351 318 306 249 232 224 214
121 111 92 89 83 77 69 60
57 42 42 37 25 22 20 15
BspEI T'CCGG_A
Cuts at: 0 1898 3378
Size: 1898 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 654 1142 1790 2179 2564 2849 3378
Size: 654 488 648 389 385 285 529
Fragments arranged by size:
654 648 529 488 389 385 285
BsrBI CCG'CTC
Cuts at: 0 1074 1772 2142 2641 3378
Size: 1074 698 370 499 737
Fragments arranged by size:
1074 737 698 499 370
BsrFI r'CCGG_y
Cuts at: 0 299 899 1234 2157 2182 3378
Size: 299 600 335 923 25 1196
Fragments arranged by size:
1196 923 600 335 299 25
BsrGI T'GTAC_A
Cuts at: 0 83 3378
Size: 83 3295
BssHII G'CGCG_C
Cuts at: 0 2116 3378
Size: 2116 1262
BssSI C'ACGA_G
Cuts at: 0 2230 2741 3378
Size: 2230 511 637
Fragments arranged by size:
2230 637 511
BstEII G'GTnAC_C
Cuts at: 0 3171 3378
Size: 3171 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 2452 3378
Size: 789 1663 926
Fragments arranged by size:
1663 926 789
BstYI r'GATC_y
Cuts at: 0 3012 3378
Size: 3012 366
Bsu36I CC'TnA_GG
Cuts at: 0 1417 2542 3287 3378
Size: 1417 1125 745 91
Fragments arranged by size:
1417 1125 745 91
BtgI C'CryG_G
Cuts at: 0 189 227 866 928 1796 2684 2820 3378
Size: 189 38 639 62 868 888 136 558
Fragments arranged by size:
888 868 639 558 189 136 62 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1418 1832 3054 3378
Size: 1418 414 1222 324
Fragments arranged by size:
1418 1222 414 324
BtsI GCAGTG_nn'
Cuts at: 0 156 586 2795 3299 3378
Size: 156 430 2209 504 79
Fragments arranged by size:
2209 504 430 156 79
EaeI y'GGCC_r
Cuts at: 0 149 1354 1553 1764 1787 2180 3378
Size: 149 1205 199 211 23 393 1198
Fragments arranged by size:
1205 1198 393 211 199 149 23
EagI C'GGCC_G
Cuts at: 0 149 1354 2180 3378
Size: 149 1205 826 1198
Fragments arranged by size:
1205 1198 826 149
EarI CTCTTCn'nnn_
Cuts at: 0 170 678 3239 3378
Size: 170 508 2561 139
Fragments arranged by size:
2561 508 170 139
EcoICRI GAG'CTC
Cuts at: 0 991 3378
Size: 991 2387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 241 406 494 639 811 1399 1641
Size: 187 54 165 88 145 172 588 242
Cuts at: 1641 1964 1986 2686 2770 3219 3378
Size: 323 22 700 84 449 159
Fragments arranged by size:
700 588 449 323 242 187 172 165
159 145 88 84 54 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 552 3378
Size: 169 383 2826
Fragments arranged by size:
2826 383 169
EcoO109I rG'GnC_Cy
Cuts at: 0 199 1131 1141 1142 1149 1173 1374 1919
Size: 199 932 10 1 7 24 201 545
Cuts at: 1919 2382 2499 2553 3378
Size: 463 117 54 825
Fragments arranged by size:
932 825 545 463 201 199 117 54
24 10 7 1
EcoRV GAT'ATC
Cuts at: 0 2010 3378
Size: 2010 1368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2913 3378
Size: 2913 465
HaeII r_GCGC'y
Cuts at: 0 48 890 1230 1806 1866 2209 2856 3378
Size: 48 842 340 576 60 343 647 522
Fragments arranged by size:
842 647 576 522 343 340 60 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 330 362 2997 3029 3340 3372
Size: 19 32 279 32 2635 32 311 32
Cuts at: 3372 3378
Size: 6
Fragments arranged by size:
2635 311 279 32 32 32 32 19
6
HindIII A'AGCT_T
Cuts at: 0 2419 3378
Size: 2419 959
KasI G'GCGC_C
Cuts at: 0 44 1862 2205 3378
Size: 44 1818 343 1173
Fragments arranged by size:
1818 1173 343 44
KpnI G_GTAC'C
Cuts at: 0 216 835 3378
Size: 216 619 2543
Fragments arranged by size:
2543 619 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 3350 3378
Size: 884 2466 28
Fragments arranged by size:
2466 884 28
MscI TGG'CCA
Cuts at: 0 1555 1789 3378
Size: 1555 234 1589
Fragments arranged by size:
1589 1555 234
MslI CAynn'nnrTG
Cuts at: 0 565 3029 3378
Size: 565 2464 349
Fragments arranged by size:
2464 565 349
MspA1I CmG'CkG
Cuts at: 0 9 426 966 1452 2075 2287 2822 3361
Size: 9 417 540 486 623 212 535 539
Cuts at: 3361 3378
Size: 17
Fragments arranged by size:
623 540 539 535 486 417 212 17
9
NaeI GCC'GGC
Cuts at: 0 2159 2184 3378
Size: 2159 25 1194
Fragments arranged by size:
2159 1194 25
NarI GG'CG_CC
Cuts at: 0 45 1863 2206 3378
Size: 45 1818 343 1172
Fragments arranged by size:
1818 1172 343 45
NcoI C'CATG_G
Cuts at: 0 227 866 3378
Size: 227 639 2512
Fragments arranged by size:
2512 639 227
NgoMIV G'CCGG_C
Cuts at: 0 2157 2182 3378
Size: 2157 25 1196
Fragments arranged by size:
2157 1196 25
NspI r_CATG'y
Cuts at: 0 2334 2723 3183 3378
Size: 2334 389 460 195
Fragments arranged by size:
2334 460 389 195
PasI CC'CwG_GG
Cuts at: 0 1343 2603 3378
Size: 1343 1260 775
Fragments arranged by size:
1343 1260 775
PflMI CCAn_nnn'nTGG
Cuts at: 0 28 763 3378
Size: 28 735 2615
Fragments arranged by size:
2615 735 28
PfoI T'CCnGG_A
Cuts at: 0 1259 2354 3008 3378
Size: 1259 1095 654 370
Fragments arranged by size:
1259 1095 654 370
PmlI CAC'GTG
Cuts at: 0 2268 2536 3378
Size: 2268 268 842
Fragments arranged by size:
2268 842 268
PpuMI rG'GwC_Cy
Cuts at: 0 199 1131 1919 2382 2553 3378
Size: 199 932 788 463 171 825
Fragments arranged by size:
932 825 788 463 199 171
PsiI TTA'TAA
Cuts at: 0 2036 3378
Size: 2036 1342
PspOMI G'GGCC_C
Cuts at: 0 18 878 1141 1373 2455 3378
Size: 18 860 263 232 1082 923
Fragments arranged by size:
1082 923 860 263 232 18
PstI C_TGCA'G
Cuts at: 0 163 1172 1750 2589 2846 3378
Size: 163 1009 578 839 257 532
Fragments arranged by size:
1009 839 578 532 257 163
PvuII CAG'CTG
Cuts at: 0 9 426 966 1452 3378
Size: 9 417 540 486 1926
Fragments arranged by size:
1926 540 486 417 9
RsrII CG'GwC_CG
Cuts at: 0 1232 3378
Size: 1232 2146
SacI G_AGCT'C
Cuts at: 0 993 3378
Size: 993 2385
SacII CC_GC'GG
Cuts at: 0 2823 3378
Size: 2823 555
SbfI CC_TGCA'GG
Cuts at: 0 1172 2846 3378
Size: 1172 1674 532
Fragments arranged by size:
1674 1172 532
SexAI A'CCwGG_T
Cuts at: 0 827 3378
Size: 827 2551
SfcI C'TryA_G
Cuts at: 0 159 1168 1209 1746 2244 2585 2803 2842
Size: 159 1009 41 537 498 341 218 39
Cuts at: 2842 3378
Size: 536
Fragments arranged by size:
1009 537 536 498 341 218 159 41
39
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 877 1172 1552 2498 3378
Size: 877 295 380 946 880
Fragments arranged by size:
946 880 877 380 295
SfoI GGC'GCC
Cuts at: 0 46 1864 2207 3378
Size: 46 1818 343 1171
Fragments arranged by size:
1818 1171 343 46
SmaI CCC'GGG
Cuts at: 0 544 629 877 1372 2154 3353 3378
Size: 544 85 248 495 782 1199 25
Fragments arranged by size:
1199 782 544 495 248 85 25
SmlI C'TyrA_G
Cuts at: 0 1008 2503 2518 2887 3061 3378
Size: 1008 1495 15 369 174 317
Fragments arranged by size:
1495 1008 369 317 174 15
SphI G_CATG'C
Cuts at: 0 2723 3378
Size: 2723 655
SrfI GCCC'GGGC
Cuts at: 0 544 2154 3378
Size: 544 1610 1224
Fragments arranged by size:
1610 1224 544
StuI AGG'CCT
Cuts at: 0 805 1330 3378
Size: 805 525 2048
Fragments arranged by size:
2048 805 525
StyI C'CwwG_G
Cuts at: 0 40 227 404 677 866 1476 1922 3378
Size: 40 187 177 273 189 610 446 1456
Fragments arranged by size:
1456 610 446 273 189 187 177 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 1356 3378
Size: 20 1336 2022
Fragments arranged by size:
2022 1336 20
TatI w'GTAC_w
Cuts at: 0 83 3378
Size: 83 3295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 732 764 841 873 3378
Size: 732 32 77 32 2505
Fragments arranged by size:
2505 732 77 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 561 3378
Size: 103 458 2817
Fragments arranged by size:
2817 458 103
XmaI C'CCGG_G
Cuts at: 0 542 627 875 1370 2152 3351 3378
Size: 542 85 248 495 782 1199 27
Fragments arranged by size:
1199 782 542 495 248 85 27
ZraI GAC'GTC
Cuts at: 0 3146 3378
Size: 3146 232
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuII RsrII SacI SacII SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AloI AscI AseI AsiSI BaeI
BamHI BciVI BfrBI BglII BmtI BsaBI BsgI BsiWI
BsmI BspHI BsrDI BstAPI BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EciI EcoRI FseI FspI FspAI
HincII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PspXI PsrI
PvuI SalI SanDI SapI ScaI SgfI SgrAI SnaBI
SpeI SspI SwaI Tth111I XbaI XhoI XmnI