Restriction map for
pFN21AA1339
(Circular) (Six-base) MAPSORT of: pFN21AA1339.seq Check: 7167 from: 1 to: 6631
pFN21AA1339 6631 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2118 3016 3209 3588 2118
Size: 898 193 379 5161
Fragments arranged by size:
5161 898 379 193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2938 4441 278
Size: 53 83 186 936 1402 1503 2468
Fragments arranged by size:
2468 1503 1402 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 3974 1937
Size: 975 1062 4594
Fragments arranged by size:
4594 1062 975
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2823 2976 3379 3604 3897 4694 6078 6346
Size: 1191 153 403 225 293 797 1384 268
Cuts at: 6346 1632
Size: 1917
Fragments arranged by size:
1917 1384 1191 797 403 293 268 225
153
AfeI AGC'GCT
Cuts at: 3308 3308
Size: 6631
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6434
AflIII A'CryG_T
Cuts at: 1203 2757 3069 5530 6507 1203
Size: 1554 312 2461 977 1327
Fragments arranged by size:
2461 1554 1327 977 312
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 3045 1166
Size: 1879 4752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6631
AlwNI CAG_nnn'CTG
Cuts at: 2945 4374 5946 2945
Size: 1429 1572 3630
Fragments arranged by size:
3630 1572 1429
ApaI G_GGCC'C
Cuts at: 2734 2734
Size: 6631
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 3940 4166 4318 6427 1493
Size: 2447 226 152 2109 1697
Fragments arranged by size:
2447 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 3956 1870
Size: 73 2013 4545
Fragments arranged by size:
4545 2013 73
BamHI G'GATC_C
Cuts at: 2382 3961 4328 2382
Size: 1579 367 4685
Fragments arranged by size:
4685 1579 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2425 3952 5407 618
Size: 325 217 627 638 1527 1455 1842
Fragments arranged by size:
1842 1527 1455 638 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2734 3349 3950 1391
Size: 1343 615 601 4072
Fragments arranged by size:
4072 1343 615 601
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5660
BbvCI CC'TCA_GC
Cuts at: 2010 3158 2010
Size: 1148 5483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4822 4856 1903
Size: 34 2885 34 3678
Fragments arranged by size:
3678 2885 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4523 5733 4523
Size: 1210 5421
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 5242 136
Size: 107 122 71 631 605 484 3086 1525
Fragments arranged by size:
3086 1525 631 605 484 122 107 71
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 4048 4048
Size: 6631
Bme1580I G_kGCm'C
Cuts at: 1790 2734 2831 3124 3131 3379 4692 5848 1790
Size: 944 97 293 7 248 1313 1156 2573
Fragments arranged by size:
2573 1313 1156 944 293 248 97 7
BmgBI CAC'GTC
Cuts at: 1318 2758 1318
Size: 1440 5191
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 3118 3373
Size: 416 53 83 399 24 1235 466 255
Cuts at: 3373 3852 4362 5320 442
Size: 479 510 958 1753
Fragments arranged by size:
1753 1235 958 510 479 466 416 399
255 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2363 2382 2451 2997 5291 1239
Size: 1061 63 19 69 546 2294 2579
Fragments arranged by size:
2579 2294 1061 546 69 63 19
Bpu10I CC'TnA_GC
Cuts at: 2010 3132 3158 2010
Size: 1122 26 5483
Fragments arranged by size:
5483 1122 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3734 4756 5621 5919 3734
Size: 1022 865 298 4446
Fragments arranged by size:
4446 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3881 5294 882
Size: 617 2382 1413 2219
Fragments arranged by size:
2382 2219 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 4422 493
Size: 711 3218 2702
Fragments arranged by size:
3218 2702 711
BsaBI GATnn'nnATC
Cuts at: 6625 6625
Size: 6631
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2935 4438 4820 5455
Size: 53 83 186 936 1402 1503 382 635
Cuts at: 5455 275
Size: 1451
Fragments arranged by size:
1503 1451 1402 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2737 3339 5064 5462 5736 5883 6294 2737
Size: 602 1725 398 274 147 411 3074
Fragments arranged by size:
3074 1725 602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 3695 3725 3909
Size: 30 165 30 870 30 1392 30 184
Cuts at: 3909 3915 3939 3945 1178
Size: 6 24 6 3864
Fragments arranged by size:
3864 1392 870 184 165 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6631
BseYI C'CCAG_C
Cuts at: 2089 2299 5834 6330 2089
Size: 210 3535 496 2390
Fragments arranged by size:
3535 2390 496 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 3526 3584 1163
Size: 573 438 1352 58 4210
Fragments arranged by size:
4210 1352 573 438 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 4842 5870 1540
Size: 275 186 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3108 3349 3579 4692 4777 5848 3108
Size: 241 230 1113 85 1071 3891
Fragments arranged by size:
3891 1113 1071 241 230 85
BsmI GAATG_Cn'
Cuts at: 4142 4235 4142
Size: 93 6538
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2734 2831 3108 3124 3131 3349 3379
Size: 399 944 97 277 16 7 218 30
Cuts at: 3379 3579 3950 4692 4777 5848 1391
Size: 200 371 742 85 1071 2174
Fragments arranged by size:
2174 1071 944 742 399 371 277 218
200 97 85 30 16 7
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 2517 4520 6250 2517
Size: 2003 1730 2898
Fragments arranged by size:
2898 2003 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2367 2958 3016 3209 3588 3986 844
Size: 1274 249 591 58 193 379 398 3489
Fragments arranged by size:
3489 1274 591 398 379 249 193 58
BsrBI CCG'CTC
Cuts at: 1394 1823 3613 3697 4518 1394
Size: 429 1790 84 821 3507
Fragments arranged by size:
3507 1790 821 429 84
BsrDI GCAATG_nn'
Cuts at: 65 1248 5126 5300 65
Size: 1183 3878 174 1396
Fragments arranged by size:
3878 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 3591 5275 5462 1700
Size: 110 377 1404 1684 187 2869
Fragments arranged by size:
2869 1684 1404 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 2760 96
Size: 2664 3967
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2876 3549 794
Size: 2082 673 3876
Fragments arranged by size:
3876 2082 673
BstBI TT'CG_AA
Cuts at: 5435 5435
Size: 6631
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstXI CCAn_nnnn'nTGG
Cuts at: 3047 3902 3047
Size: 855 5776
BstYI r'GATC_y
Cuts at: 1972 2178 2382 2816 3961 4328 4713 4730 6182
Size: 206 204 434 1145 367 385 17 1452
Cuts at: 6182 6268 6280 6626 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1145 434 385 367 346 206
204 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2721 2832 2721
Size: 111 6520
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 4597
Fragments arranged by size:
4597 939 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 4147 4941 4961 4147
Size: 794 20 5817
Fragments arranged by size:
5817 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 3935 3935
Size: 6631
DraIII CAC_nnn'GTG
Cuts at: 3212 3212
Size: 6631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5638 809
Size: 507 4322 1802
Fragments arranged by size:
4322 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 3484 4078
Size: 54 1425 50 275 18 315 1339 594
Cuts at: 4078 4967 6596 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1339 889 594 315 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2658 2709 2778 2857 4561 1353
Size: 729 576 51 69 79 1704 3423
Fragments arranged by size:
3423 1704 729 576 79 69 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3167 3491 5218 5592 5738 387
Size: 1064 138 1578 324 1727 374 146 1280
Fragments arranged by size:
1727 1578 1280 1064 374 324 146 138
EcoICRI GAG'CTC
Cuts at: 3347 3347
Size: 6631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2363 2382 2451 2823 2976 2997
Size: 393 668 63 19 69 372 153 21
Cuts at: 2997 3379 3604 3897 4694 5291 6078 6346 1239
Size: 382 225 293 797 597 787 268 1524
Fragments arranged by size:
1524 797 787 668 597 393 382 372
293 268 225 153 69 63 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2312 2312
Size: 6631
EcoO109I rG'GnC_Cy
Cuts at: 2730 2731 3363 3830 2730
Size: 1 632 467 5531
Fragments arranged by size:
5531 632 467 1
EcoRI G'AATT_C
Cuts at: 3940 4318 3940
Size: 378 6253
EcoRV GAT'ATC
Cuts at: 2416 2416
Size: 6631
FspI TGC'GCA
Cuts at: 2366 3569 5137 2366
Size: 1203 1568 3860
Fragments arranged by size:
3860 1568 1203
HaeII r_GCGC'y
Cuts at: 1678 3310 3448 5778 1678
Size: 1632 138 2330 2531
Fragments arranged by size:
2531 2330 1632 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2432 2464 2564 2596 2678
Size: 32 135 32 860 32 100 32 82
Cuts at: 2678 2710 2968 3000 3211 3243 5272 5304 5346
Size: 32 258 32 211 32 2029 32 42
Cuts at: 5346 5378 1373
Size: 32 2626
Fragments arranged by size:
2626 2029 860 258 211 135 100 82
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3871 3975 4221 669
Size: 988 281 1933 104 246 3079
Fragments arranged by size:
3079 1933 988 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6631
HpaI GTT'AAC
Cuts at: 4221 4221
Size: 6631
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 4230 4230
Size: 6631
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2192 5745 5929 1305
Size: 887 3553 184 2007
Fragments arranged by size:
3553 2007 887 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4809
Fragments arranged by size:
4809 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2035 2546 3045 4589 4948 5107 518
Size: 648 869 511 499 1544 359 159 2042
Fragments arranged by size:
2042 1544 869 648 511 499 359 159
MspA1I CmG'CkG
Cuts at: 1606 2244 2278 3372 3427 4047 4724 5872 6117
Size: 638 34 1094 55 620 677 1148 245
Cuts at: 6117 1606
Size: 2120
Fragments arranged by size:
2120 1148 1094 677 638 620 245 55
34
NaeI GCC'GGC
Cuts at: 2189 2189
Size: 6631
NcoI C'CATG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 4597
Fragments arranged by size:
4597 939 552 543
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NgoMIV G'CCGG_C
Cuts at: 2187 2187
Size: 6631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6631
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 3073 3989 5534 1132
Size: 97 1844 916 1545 2229
Fragments arranged by size:
2229 1844 1545 916 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6631
PciI A'CATG_T
Cuts at: 3069 5530 3069
Size: 2461 4170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3897 1108
Size: 2789 3842
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 4700 4732 6213 6245 3014
Size: 32 1654 32 1481 32 3400
Fragments arranged by size:
3400 1654 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3830 3830
Size: 6631
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6631
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PspOMI G'GGCC_C
Cuts at: 2730 2730
Size: 6631
PstI C_TGCA'G
Cuts at: 830 2067 2115 2316 2334 3554 3983 830
Size: 1237 48 201 18 1220 429 3478
Fragments arranged by size:
3478 1237 1220 429 201 48 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
PvuII CAG'CTG
Cuts at: 2244 2278 2244
Size: 34 6597
SacI G_AGCT'C
Cuts at: 3349 3349
Size: 6631
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SapI GCTCTTCn'nnn_
Cuts at: 2658 2709 2778 2658
Size: 51 69 6511
Fragments arranged by size:
6511 69 51
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 4879 6591 4879
Size: 1712 4919
SexAI A'CCwGG_T
Cuts at: 3826 3826
Size: 6631
SfcI C'TryA_G
Cuts at: 826 1045 2063 2111 2160 2312 2330 3550 3979
Size: 219 1018 48 49 152 18 1220 429
Cuts at: 3979 5114 5795 5986 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1220 1135 1018 681 429 219 191
152 49 48 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 3591 1700
Size: 1891 4740
SmaI CCC'GGG
Cuts at: 1872 3958 1872
Size: 2086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3749 4735 5636 5898 820
Size: 197 926 1806 986 901 262 1553
Fragments arranged by size:
1806 1553 986 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4422 493
Size: 3929 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 4555 5
Size: 47 4503 2081
Fragments arranged by size:
4503 2081 47
StuI AGG'CCT
Cuts at: 1517 2808 1517
Size: 1291 5340
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2745 4070 513
Size: 552 939 543 198 1325 3074
Fragments arranged by size:
3074 1325 939 552 543 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3246 3832 3854 4658 4675 4828 5013
Size: 1255 1666 586 22 804 17 153 185
Cuts at: 5013 5458 325
Size: 445 1498
Fragments arranged by size:
1666 1498 1255 804 586 445 185 153
22 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2760 4877 6589
Size: 274 80 33 51 1443 783 2117 1712
Cuts at: 6589 96
Size: 138
Fragments arranged by size:
2117 1712 1443 783 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 2273 2305 3262 3294 6591 6623 2033
Size: 32 208 32 957 32 3297 32 2041
Fragments arranged by size:
3297 2041 957 208 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 3883 3967 3883
Size: 84 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 3956 1870
Size: 2086 4545
XmnI GAAnn'nnTTC
Cuts at: 4760 4760
Size: 6631
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2936 4439 276
Size: 53 83 186 936 1402 1503 2468
Fragments arranged by size:
2468 1503 1402 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI XcmI