(Circular) (Six-base) MAPSORT of: pFN21AE0935.seq Check: 4206 from: 1 to: 5959 pFN21AE0935 5959 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2043 3302 1937 Size: 106 1259 4594 Fragments arranged by size: 4594 1259 106 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 4022 5406 5674 1632 Size: 662 1728 1384 268 1917 Fragments arranged by size: 1917 1728 1384 662 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 2754 2838 1166 Size: 1588 84 4287 Fragments arranged by size: 4287 1588 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2283 2510 2661 2745 2829 2913 3081 3165 Size: 790 227 151 84 84 84 168 84 Cuts at: 3165 3247 3268 3494 3646 5755 1493 Size: 82 21 226 152 2109 1697 Fragments arranged by size: 2109 1697 790 227 226 168 152 151 84 84 84 84 82 21 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2196 2975 3278 1391 Size: 805 779 303 4072 Fragments arranged by size: 4072 805 779 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2080 1548 Size: 532 5427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2446 5954 2446 Size: 3508 2451 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 2336 1318 Size: 1018 4941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 2931 3099 Size: 416 53 83 399 24 882 632 168 Cuts at: 3099 3265 3690 4648 442 Size: 166 425 958 1753 Fragments arranged by size: 1753 958 882 632 425 416 399 168 166 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2335 4619 1239 Size: 1096 2284 2579 Fragments arranged by size: 2579 2284 1096 Bpu10I CC'TnA_GC Cuts at: 2010 2010 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2623 4084 4949 5247 2623 Size: 1461 865 298 3335 Fragments arranged by size: 3335 1461 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2249 4622 882 Size: 617 750 2373 2219 Fragments arranged by size: 2373 2219 750 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 1178 Size: 30 165 30 780 30 4924 Fragments arranged by size: 4924 780 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5073 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2351 2975 4020 4105 5176 2351 Size: 624 1045 85 1071 3134 Fragments arranged by size: 3134 1071 1045 624 85 BsmI GAATG_Cn' Cuts at: 2072 2143 2861 2870 3470 3563 2072 Size: 71 718 9 600 93 4468 Fragments arranged by size: 4468 718 600 93 71 9 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2351 2975 3278 4020 4105 5176 Size: 399 406 155 624 303 742 85 1071 Cuts at: 5176 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 624 406 399 303 155 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2440 3848 5578 2440 Size: 1408 1730 2821 Fragments arranged by size: 2821 1730 1408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2962 4454 4628 65 Size: 1183 1714 1492 174 1396 Fragments arranged by size: 1714 1492 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 2865 794 Size: 2071 3888 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2446 3289 3656 4041 4058 5510 5596 5608 Size: 474 843 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 843 474 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2231 3406 4295 Size: 54 1425 50 275 18 401 1175 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1175 889 401 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3889 1353 Size: 2536 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2973 2973 Size: 5959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2335 4022 4619 5406 5674 1239 Size: 393 662 41 1687 597 787 268 1524 Fragments arranged by size: 1687 1524 787 662 597 393 268 41 EcoRI G'AATT_C Cuts at: 2745 2829 2913 3081 3165 3268 3646 2745 Size: 84 84 168 84 103 378 5058 Fragments arranged by size: 5058 378 168 103 84 84 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 4600 4632 4674 Size: 32 135 32 880 32 2116 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 2116 880 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 2426 3558 2426 Size: 1132 4827 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5073 5257 1305 Size: 786 2982 184 2007 Fragments arranged by size: 2982 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2233 10 Size: 54 1425 343 401 3736 Fragments arranged by size: 3736 1425 401 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2754 2838 3006 3174 3917 4276 4435 Size: 648 1588 84 168 168 743 359 159 Cuts at: 4435 518 Size: 2042 Fragments arranged by size: 2042 1588 743 648 359 168 168 159 84 MspA1I CmG'CkG Cuts at: 1606 3375 4052 5200 5445 1606 Size: 1769 677 1148 245 2120 Fragments arranged by size: 2120 1769 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2238 2238 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacI G_AGCT'C Cuts at: 2975 2975 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SexAI A'CCwGG_T Cuts at: 2127 2127 Size: 5959 SfcI C'TryA_G Cuts at: 826 1045 3307 4442 5123 5314 826 Size: 219 2262 1135 681 191 1471 Fragments arranged by size: 2262 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2638 4063 4964 5226 820 Size: 197 926 695 1425 901 262 1553 Fragments arranged by size: 1553 1425 926 901 695 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 3173 152 Size: 3021 2938 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2876 1517 Size: 1359 4600 StyI C'CwwG_G Cuts at: 513 1065 2004 3398 513 Size: 552 939 1394 3074 Fragments arranged by size: 3074 1394 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3986 4003 4156 4341 4786 325 Size: 1255 2406 17 153 185 445 1498 Fragments arranged by size: 2406 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 4205 5917 Size: 274 80 33 51 1443 1275 953 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1443 1275 953 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2456 2488 5919 5951 2456 Size: 32 3431 32 2464 Fragments arranged by size: 3431 2464 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI