(Circular) (Six-base) MAPSORT of: pFN21AB4886.seq Check: 6138 from: 1 to: 5725 pFN21AB4886 5725 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 2436 3046 2436 Size: 610 5115 AclI AA'CG_TT Cuts at: 1563 3024 3852 4225 1563 Size: 1461 828 373 3063 Fragments arranged by size: 3063 1461 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 2600 1166 Size: 1434 4291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2957 3050 1870 Size: 73 1014 93 4545 Fragments arranged by size: 4545 1014 93 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2163 2436 2609 3046 4501 Size: 325 217 627 376 273 173 437 1455 Cuts at: 4501 618 Size: 1842 Fragments arranged by size: 1842 1455 627 437 376 325 273 217 173 BanII G_rGCy'C Cuts at: 1391 2787 2996 3044 1391 Size: 1396 209 48 4072 Fragments arranged by size: 4072 1396 209 48 BbeI G_GCGC'C Cuts at: 2613 2613 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2675 2709 3916 3950 1903 Size: 34 738 34 1207 34 3678 Fragments arranged by size: 3678 1207 738 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2485 3617 4827 2485 Size: 1132 1210 3383 Fragments arranged by size: 3383 1210 1132 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2175 3015 5720 2175 Size: 840 2705 2180 Fragments arranged by size: 2705 2180 840 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2166 2506 3786 4942 1790 Size: 376 340 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 376 340 BmgBI CAC'GTC Cuts at: 1318 2641 1318 Size: 1323 4402 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2200 2442 Size: 416 53 83 399 24 697 86 242 Cuts at: 2442 3456 4414 442 Size: 1014 958 1753 Fragments arranged by size: 1753 1014 958 697 416 399 242 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 4385 1239 Size: 797 2349 2579 Fragments arranged by size: 2579 2349 797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 2366 2996 3850 4715 5013 2188 Size: 178 630 854 865 298 2900 Fragments arranged by size: 2900 865 854 630 298 178 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2751 3516 493 Size: 711 1547 765 2702 Fragments arranged by size: 2702 1547 765 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2610 2745 3532 3914 Size: 53 83 186 936 1077 135 787 382 Cuts at: 3914 4549 275 Size: 635 1451 Fragments arranged by size: 1451 1077 936 787 635 382 186 135 83 53 BsaWI w'CCGG_w Cuts at: 2046 4158 4556 4830 4977 5388 2046 Size: 2112 398 274 147 411 2383 Fragments arranged by size: 2383 2112 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 4522 Fragments arranged by size: 4522 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 2777 1186 Size: 1098 493 4134 Fragments arranged by size: 4134 1098 493 BseYI C'CCAG_C Cuts at: 2108 4928 5424 2108 Size: 2820 496 2409 Fragments arranged by size: 2820 2409 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2937 1163 Size: 573 1201 3951 Fragments arranged by size: 3951 1201 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3175 3936 4964 1540 Size: 275 186 913 261 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 261 186 BsiHKAI G_wGCw'C Cuts at: 2787 3786 3871 4942 2787 Size: 999 85 1071 3570 Fragments arranged by size: 3570 1071 999 85 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2506 2787 2996 3044 3786 3871 Size: 399 376 340 281 209 48 742 85 Cuts at: 3871 4942 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 340 281 209 85 48 BspEI T'CCGG_A Cuts at: 2046 5388 2046 Size: 3342 2383 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2775 3080 844 Size: 1931 305 3489 Fragments arranged by size: 3489 1931 305 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2732 4369 4556 1700 Size: 110 922 1637 187 2869 Fragments arranged by size: 2869 1637 922 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 2962 1184 Size: 1778 3947 BstYI r'GATC_y Cuts at: 1972 2055 2175 3015 3055 3422 3807 3824 5276 Size: 83 120 840 40 367 385 17 1452 Cuts at: 5276 5362 5374 5720 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 840 385 367 346 120 86 83 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2326 2326 Size: 5725 BtgI C'CryG_G Cuts at: 513 1065 2158 2988 513 Size: 552 1093 830 3250 Fragments arranged by size: 3250 1093 830 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2703 3241 4035 4055 2703 Size: 538 794 20 4373 Fragments arranged by size: 4373 794 538 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2425 2590 2674 Size: 54 1425 50 275 18 595 165 84 Cuts at: 2674 2932 2985 3172 4061 5690 8 Size: 258 53 187 889 1629 43 Fragments arranged by size: 1629 1425 889 595 275 258 187 165 84 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2765 3655 1353 Size: 1412 890 3423 Fragments arranged by size: 3423 1412 890 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2785 2785 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 3788 4385 5172 5440 1239 Size: 393 404 1752 597 787 268 1524 Fragments arranged by size: 1752 1524 787 597 404 393 268 EcoO109I rG'GnC_Cy Cuts at: 2142 2151 2142 Size: 9 5716 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2223 2613 4872 1678 Size: 545 390 2259 2531 Fragments arranged by size: 2531 2259 545 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 4366 4398 4440 Size: 32 135 32 980 32 1782 32 42 Cuts at: 4440 4472 1373 Size: 32 2626 Fragments arranged by size: 2626 1782 980 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2609 2609 Size: 5725 KpnI G_GTAC'C Cuts at: 2440 3050 2440 Size: 610 5115 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2334 2709 4839 5023 1305 Size: 1029 375 2130 184 2007 Fragments arranged by size: 2130 2007 1029 375 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2427 2934 2987 10 Size: 54 1425 343 595 507 53 2748 Fragments arranged by size: 2748 1425 595 507 343 54 53 MslI CAynn'nnrTG Cuts at: 518 1166 2600 2981 3683 4042 4201 518 Size: 648 1434 381 702 359 159 2042 Fragments arranged by size: 2042 1434 702 648 381 359 159 MspA1I CmG'CkG Cuts at: 1606 2108 2287 2847 3141 3818 4966 5211 1606 Size: 502 179 560 294 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 560 502 294 245 179 NarI GG'CG_CC Cuts at: 2610 2610 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2079 3083 4628 1132 Size: 97 850 1004 1545 2229 Fragments arranged by size: 2229 1545 1004 850 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2190 1108 Size: 1082 4643 PfoI T'CCnGG_A Cuts at: 2058 2457 2058 Size: 399 5326 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PmlI CAC'GTG Cuts at: 2751 2751 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2142 2151 2142 Size: 9 5716 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2108 2108 Size: 5725 SacI G_AGCT'C Cuts at: 2787 2787 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2374 3073 4208 4889 5080 826 Size: 219 1329 699 1135 681 191 1471 Fragments arranged by size: 1471 1329 1135 699 681 219 191 SfoI GGC'GCC Cuts at: 2611 2611 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2203 2381 3011 3829 4730 4992 Size: 197 926 260 178 630 818 901 262 Cuts at: 4992 820 Size: 1553 Fragments arranged by size: 1553 926 901 818 630 262 260 197 178 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 3164 513 Size: 552 2099 3074 Fragments arranged by size: 3074 2099 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 3752 3769 3922 4107 4552 325 Size: 1255 633 1539 17 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 633 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2793 3971 5683 Size: 274 80 33 51 1443 816 1178 1712 Cuts at: 5683 96 Size: 138 Fragments arranged by size: 1712 1443 1178 816 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 2639 2671 5685 5717 2143 Size: 32 464 32 3014 32 2151 Fragments arranged by size: 3014 2151 464 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2105 2011 Size: 94 5631 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI