(Circular) (Six-base) MAPSORT of: pFN21AB4917.seq Check: 3956 from: 1 to: 5674 pFN21AB4917 5674 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2084 2357 2084 Size: 273 5401 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2919 3017 1937 Size: 982 98 4594 Fragments arranged by size: 4594 982 98 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2405 2756 2879 3737 5121 5389 1632 Size: 773 351 123 858 1384 268 1917 Fragments arranged by size: 1917 1384 858 773 351 268 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 2682 4573 5550 1203 Size: 1479 1891 977 1327 Fragments arranged by size: 1891 1479 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 2078 1166 Size: 912 4762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2305 3417 4989 2305 Size: 1112 1572 2990 Fragments arranged by size: 2990 1572 1112 ApaI G_GGCC'C Cuts at: 2846 2846 Size: 5674 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2983 3209 3361 5470 1493 Size: 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2237 2573 2999 1870 Size: 73 294 336 426 4545 Fragments arranged by size: 4545 426 336 294 73 BamHI G'GATC_C Cuts at: 2805 3004 3371 2805 Size: 199 367 5108 Fragments arranged by size: 5108 367 199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 2468 2864 2995 4450 Size: 325 217 627 558 123 396 131 1455 Cuts at: 4450 618 Size: 1842 Fragments arranged by size: 1842 1455 627 558 396 325 217 131 123 BanII G_rGCy'C Cuts at: 1391 2846 2993 1391 Size: 1455 147 4072 Fragments arranged by size: 4072 1455 147 BbeI G_GCGC'C Cuts at: 2868 2868 Size: 5674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2759 928 Size: 971 860 3843 Fragments arranged by size: 3843 971 860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2726 4285 136 Size: 107 122 71 631 605 1054 1559 1525 Fragments arranged by size: 1559 1525 1054 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 3091 3091 Size: 5674 Bme1580I G_kGCm'C Cuts at: 1790 2473 2846 3735 4891 1790 Size: 683 373 889 1156 2573 Fragments arranged by size: 2573 1156 889 683 373 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4363 442 Size: 416 53 83 399 24 1988 958 1753 Fragments arranged by size: 1988 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 2747 4334 1239 Size: 1236 272 1587 2579 Fragments arranged by size: 2579 1587 1236 272 Bpu10I CC'TnA_GC Cuts at: 2588 2588 Size: 5674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3799 4664 4962 3799 Size: 865 298 4511 Fragments arranged by size: 4511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 4337 882 Size: 617 1065 1773 2219 Fragments arranged by size: 2219 1773 1065 617 BsaAI yAC'GTr Cuts at: 493 1204 2683 3465 493 Size: 711 1479 782 2702 Fragments arranged by size: 2702 1479 782 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2657 2781 2865 3481 Size: 53 83 186 936 1124 124 84 616 Cuts at: 3481 3863 4498 275 Size: 382 635 1451 Fragments arranged by size: 1451 1124 936 635 616 382 186 124 84 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2199 2229 1178 Size: 30 165 30 796 30 4623 Fragments arranged by size: 4623 796 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 1186 Size: 1492 4182 BseYI C'CCAG_C Cuts at: 2472 2517 2644 2708 2947 4877 5373 2472 Size: 45 127 64 239 1930 496 2773 Fragments arranged by size: 2773 1930 496 239 127 64 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 3820 4891 3735 Size: 85 1071 4518 Fragments arranged by size: 4518 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2473 2846 2993 3735 3820 4891 1391 Size: 399 683 373 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 399 373 147 85 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2084 2357 2541 3029 844 Size: 1240 273 184 488 3489 Fragments arranged by size: 3489 1240 488 273 184 BsrBI CCG'CTC Cuts at: 1394 1823 2178 2623 3561 1394 Size: 429 355 445 938 3507 Fragments arranged by size: 3507 938 445 429 355 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 2550 4169 4343 65 Size: 1183 1164 138 1619 174 1396 Fragments arranged by size: 1619 1396 1183 1164 174 138 BsrFI r'CCGG_y Cuts at: 1700 1810 2555 2696 4318 4505 1700 Size: 110 745 141 1622 187 2869 Fragments arranged by size: 2869 1622 745 187 141 110 BsrGI T'GTAC_A Cuts at: 96 2139 96 Size: 2043 3631 BssHII G'CGCG_C Cuts at: 1925 2877 1925 Size: 952 4722 BssSI C'ACGA_G Cuts at: 2143 2837 3734 4746 2143 Size: 694 897 1012 3071 Fragments arranged by size: 3071 1012 897 694 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2095 2254 2374 2095 Size: 159 120 5395 Fragments arranged by size: 5395 159 120 BstYI r'GATC_y Cuts at: 1972 2388 2430 2805 2826 3004 3371 3756 3773 Size: 416 42 375 21 178 367 385 17 Cuts at: 3773 5225 5311 5323 5669 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 416 385 375 367 346 178 86 42 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2373 2508 2545 513 Size: 552 939 369 135 37 3642 Fragments arranged by size: 3642 939 552 369 135 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 2872 2890 2917 487 Size: 683 56 812 834 18 27 3244 Fragments arranged by size: 3244 834 812 683 56 27 18 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2128 2511 Size: 54 1425 50 275 18 267 31 383 Cuts at: 2511 2694 2727 3121 4010 5639 8 Size: 183 33 394 889 1629 43 Fragments arranged by size: 1629 1425 889 394 383 275 267 183 54 50 43 33 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3604 1353 Size: 2251 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2475 2747 2756 2879 3737 4334 Size: 393 773 70 272 9 123 858 597 Cuts at: 4334 5121 5389 1239 Size: 787 268 1524 Fragments arranged by size: 1524 858 787 773 597 393 272 268 123 70 9 EcoO109I rG'GnC_Cy Cuts at: 2842 2843 2962 2842 Size: 1 119 5554 Fragments arranged by size: 5554 119 1 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 2868 4821 1678 Size: 1190 1953 2531 Fragments arranged by size: 2531 1953 1190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2268 2300 2654 2686 2783 Size: 32 135 32 696 32 354 32 97 Cuts at: 2783 2815 4315 4347 4389 4421 1373 Size: 32 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 696 354 135 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 2785 3018 3264 669 Size: 988 281 796 51 233 246 3079 Fragments arranged by size: 3079 988 796 281 246 233 51 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KasI G'GCGC_C Cuts at: 2864 2864 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 2299 4788 4972 1305 Size: 873 121 2489 184 2007 Fragments arranged by size: 2489 2007 873 184 121 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 10 Size: 54 1425 343 267 3585 Fragments arranged by size: 3585 1425 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2147 2372 2378 3632 3991 4150 Size: 648 912 69 225 6 1254 359 159 Cuts at: 4150 518 Size: 2042 Fragments arranged by size: 2042 1254 912 648 359 225 159 69 6 MspA1I CmG'CkG Cuts at: 1606 2395 2495 2521 2644 2723 3090 3767 4915 Size: 789 100 26 123 79 367 677 1148 Cuts at: 4915 5160 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 789 677 367 245 123 100 79 26 NaeI GCC'GGC Cuts at: 2698 2698 Size: 5674 NarI GG'CG_CC Cuts at: 2865 2865 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 2373 513 Size: 552 939 369 3814 Fragments arranged by size: 3814 939 552 369 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NgoMIV G'CCGG_C Cuts at: 2696 2696 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 2152 2200 3032 4577 1132 Size: 97 923 48 832 1545 2229 Fragments arranged by size: 2229 1545 923 832 97 48 PasI CC'CwG_GG Cuts at: 1348 2443 2461 1348 Size: 1095 18 4561 Fragments arranged by size: 4561 1095 18 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5674 PfoI T'CCnGG_A Cuts at: 2808 2808 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2962 2962 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 2228 1613 Size: 615 5059 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PspOMI G'GGCC_C Cuts at: 2842 2842 Size: 5674 PstI C_TGCA'G Cuts at: 830 2115 2400 2961 3026 830 Size: 1285 285 561 65 3478 Fragments arranged by size: 3478 1285 561 285 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2395 2495 2521 2644 2395 Size: 100 26 123 5425 Fragments arranged by size: 5425 123 100 26 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2111 2396 2957 3022 4157 4838 5029 Size: 219 1066 285 561 65 1135 681 191 Cuts at: 5029 826 Size: 1471 Fragments arranged by size: 1471 1135 1066 681 561 285 219 191 65 SfoI GGC'GCC Cuts at: 2866 2866 Size: 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3778 4679 4941 820 Size: 197 926 1835 901 262 1553 Fragments arranged by size: 1835 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 2373 3113 513 Size: 552 939 369 740 3074 Fragments arranged by size: 3074 939 740 552 369 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2531 3701 3718 3871 4056 4501 325 Size: 1255 951 1170 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1170 951 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 2613 3920 Size: 274 80 33 51 1443 162 474 1307 Cuts at: 3920 5632 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1307 474 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2199 2231 5634 5666 2199 Size: 32 3403 32 2207 Fragments arranged by size: 3403 2207 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI