Restriction map for
pFN21AE0784
##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AfaI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseRI BsiWI BsmI Bsp1407I
BspDI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MslI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 293 GTATAC GT^[AC][GT]AC
AccIII 582 TCCGGA T^CCGGA
AciI 1130 CCGC CCGC(-3/-1)
AflII 909 CTTAAG C^TTAAG
AflIII 1211 ACATGT A^C[GA][CT]GT
AspHI 236 GAGCAC G[AT]GC[AT]^C
AvaI 77 CTCGAG C^[CT]CG[GA]G
BanII 220 GAGCCC G[GA]GC[CT]^C
BbvI 1220 GCTGC GCAGC(8/12)
BfrI 909 CTTAAG C^TTAAG
BsaJI 742 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 582 TCCGGA [AT]^CCGG[AT]
BseAI 582 TCCGGA T^CCGGA
BsgI 1004 GTGCAG GTGCAG(16/14)
BsiEI 61 CGATCG CG[GA][CT]^CG
BsiHKAI 236 GAGCAC G[AT]GC[AT]^C
BsmBI 686 GAGACG CGTCTC(1/5)
BsoBI 77 CTCGAG C^[CT]CG[GA]G
BspEI 582 TCCGGA T^CCGGA
BspHI 419 TCATGA T^CATGA
BspLU11I 1211 ACATGT A^CATGT
Bst1107I 293 GTATAC GTA^TAC
BstZ17I 293 GTATAC GTA^TAC
CfoI 1260 GCGC GCG^C
DraII 357 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 50 GACCTAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 683 GAAGAG CTCTTC(1/4)
EcoNI 742 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 357 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 742 CCTTGG C^C[AT][AT]GG
Fnu4HI 1220 GCTGC GC^[ACGT]GC
HaeII 1259 AGCGCC [GA]GCGC^[CT]
HaeIII 316 GGCC GG^CC
HapII 583 CCGG C^CGG
HhaI 1260 GCGC GCG^C
HinP1I 1260 GCGC G^CGC
HinfI 263 GATTC G^A[ACGT]TC
HpaII 583 CCGG C^CGG
ItaI 1220 GCTGC GC^[ACGT]GC
Ksp632I 683 GAAGAG CTCTTC(1/4)
MaeII 1137 ACGT A^CGT
MaeIII 389 GTGAC ^GT[ACGT]AC
MroI 582 TCCGGA T^CCGGA
MspI 583 CCGG C^CGG
MwoI 345 GCTGGAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 709 CATATG CA^TATG
NspI 1211 ACATGT [GA]CATG^[CT]
PaeR7I 77 CTCGAG C^TCGAG
PpuMI 357 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 61 CGATCG CGAT^CG
RcaI 419 TCATGA T^CATGA
SexAI 1082 ACCAGGT A^CC[AT]GGT
SfaNI 1065 GATGC GCATC(5/9)
StyI 742 CCTTGG C^C[AT][AT]GG
TfiI 263 GATTC G^A[AT]TC
TseI 1220 GCTGC G^C[AT]GC
Tsp45I 389 GTGAC ^GT[GC]AC
TspRI 998 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 77 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 183 AAATTT [GA]^AATT[CT]
834 AAATTT
ApoI 183 AAATTT [GA]^AATT[CT]
834 AAATTT
Asp700I 395 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
832 GAAAATTTTC
AvaII 118 GGACC G^G[AT]CC
358 GGACC
BmyI 220 GAGCCC G[AGT]GC[ACT]^C
236 GAGCAC
BsiYI 742 CCTTGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1126 CCCACCGCAGG
BslI 742 CCTTGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1126 CCCACCGCAGG
BsmAI 586 GAGAC GTCTC(1/5)
686 GAGAC
BsmFI 66 GGGAC GGGAC(10/14)
357 GGGAC
Bsp1286I 220 GAGCCC G[AGT]GC[ACT]^C
236 GAGCAC
BssKI 517 CCAGG ^CC[ACGT]GG
1083 CCAGG
BstF5I 148 GGATG GGATG(2/0)
1182 GGATG
BstNI 517 CCAGG CC^[AT]GG
1083 CCAGG
BstYI 280 GGATCT [GA]^GATC[CT]
1017 AGATCC
DdeI 494 CTGAG C^T[ACGT]AG
727 CTGAG
EcoRII 517 CCAGG ^CC[AT]GG
1083 CCAGG
FokI 148 GGATG GGATG(9/13)
1182 GGATG
HindIII 698 AAGCTT A^AGCTT
916 AAGCTT
MflI 280 GGATCT [GA]^GATC[CT]
1017 AGATCC
MvaI 517 CCAGG CC^[AT]GG
1083 CCAGG
NlaIV 219 GGAGCC GG[ACGT]^[ACGT]CC
357 GGGACC
ScrFI 517 CCAGG CC^[ACGT]GG
1083 CCAGG
XhoII 280 GGATCT [GA]^GATC[CT]
1017 AGATCC
XmnI 395 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
832 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AlwI BbsI BfaI BpmI
BpuAI BsrI Cfr13I DpnII MaeI
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI Sau96I SmlI TaqI
Tru9I Tsp509I TthHB8I