Restriction map for
pFN21AE0784
(Circular) (Six-base) MAPSORT of: pFN21AE0784.seq Check: 2211 from: 1 to: 5974
pFN21AE0784 5974 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2299 3317 1937
Size: 362 1018 4594
Fragments arranged by size:
4594 1018 362
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3192 4037 5421 5689 1632
Size: 1560 845 1384 268 1917
Fragments arranged by size:
1917 1560 1384 845 268
AflII C'TTAA_G
Cuts at: 820 1017 2914 820
Size: 197 1897 3880
Fragments arranged by size:
3880 1897 197
AflIII A'CryG_T
Cuts at: 1203 3216 4873 5850 1203
Size: 2013 1657 977 1327
Fragments arranged by size:
2013 1657 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3042 1184
Size: 1858 4116
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2188 2839 3283 3509 3661 5770 1493
Size: 695 651 444 226 152 2109 1697
Fragments arranged by size:
2109 1697 695 651 444 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 3299 1870
Size: 73 139 1217 4545
Fragments arranged by size:
4545 1217 139 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 3293 1391
Size: 838 1064 4072
Fragments arranged by size:
4072 1064 838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2382 2735 928
Size: 971 189 294 353 4167
Fragments arranged by size:
4167 971 353 294 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 3705 4663
Size: 416 53 83 399 24 683 1605 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1605 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2310 2985 4634 1239
Size: 888 183 675 1649 2579
Fragments arranged by size:
2579 1649 888 675 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2289 4099 4964 5262 2289
Size: 1810 865 298 3001
Fragments arranged by size:
3001 1810 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2587 4407 4805 5079 5226 5637 2587
Size: 1820 398 274 147 411 2924
Fragments arranged by size:
2924 1820 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5974
BseYI C'CCAG_C
Cuts at: 2359 2535 5177 5673 2359
Size: 176 2642 496 2660
Fragments arranged by size:
2660 2642 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3030 1163
Size: 573 1294 4107
Fragments arranged by size:
4107 1294 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 3424 4185 5213 1540
Size: 275 186 68 1355 761 1028 2301
Fragments arranged by size:
2301 1355 1028 761 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2245 4035 4120 5191 2245
Size: 1790 85 1071 3028
Fragments arranged by size:
3028 1790 1071 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
BsmBI CGTCTCn'nnnn_
Cuts at: 2685 2685
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2245 3293 4035 4120 5191 1391
Size: 399 439 16 1048 742 85 1071 2174
Fragments arranged by size:
2174 1071 1048 742 439 399 85 16
BspEI T'CCGG_A
Cuts at: 2587 5637 2587
Size: 3050 2924
BspHI T'CATG_A
Cuts at: 2424 3863 5593 2424
Size: 1439 1730 2805
Fragments arranged by size:
2805 1730 1439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2285 3022 3304 3671 4056 4073 5525 5611
Size: 313 737 282 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 737 385 367 346 313 282
86 17 12
BstZ17I GTA'TAC
Cuts at: 2300 2300
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 3490 4284 4304 3490
Size: 794 20 5160
Fragments arranged by size:
5160 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2061 4981 809
Size: 507 745 2920 1802
Fragments arranged by size:
2920 1802 745 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2683 3904 1353
Size: 1330 1221 3423
Fragments arranged by size:
3423 1330 1221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2310 2985 3192 4037 4634 5421
Size: 393 495 183 675 207 845 597 787
Cuts at: 5421 5689 1239
Size: 268 1524
Fragments arranged by size:
1524 845 787 675 597 495 393 268
207 183
EcoNI CCTnn'n_nnAGG
Cuts at: 2751 2751
Size: 5974
EcoO109I rG'GnC_Cy
Cuts at: 2363 2363
Size: 5974
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 3268 5121 1678
Size: 1590 1853 2531
Fragments arranged by size:
2531 1853 1590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2725 2757 4615 4647 4689
Size: 32 135 32 1153 32 1858 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 1858 1153 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2703 2921 748
Size: 1955 218 3801
Fragments arranged by size:
3801 1955 218
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2101 2119 2202 5088 5272 1305
Size: 796 18 83 2886 184 2007
Fragments arranged by size:
2886 2007 796 184 83 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4291 4450 518
Size: 648 2766 359 159 2042
Fragments arranged by size:
2766 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3390 4067 5215 5460 1606
Size: 1784 677 1148 245 2120
Fragments arranged by size:
2120 1784 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 2715 387
Size: 2328 3646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3220 3332 4877 1132
Size: 97 1991 112 1545 2229
Fragments arranged by size:
2229 1991 1545 112 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 3216 4873 3216
Size: 1657 4317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2424 4043 4075 5556 5588 2392
Size: 32 1619 32 1481 32 2778
Fragments arranged by size:
2778 1619 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2363 2363
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspXI vC'TCGA_Gb
Cuts at: 2082 2082
Size: 5974
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 5942
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2069 2001
Size: 68 5906
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 3087 3087
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 3322 4457 5138 5329 826
Size: 219 2277 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2082 2304 2914 4078 4979 5241
Size: 197 926 139 222 610 1164 901 262
Cuts at: 5241 820
Size: 1553
Fragments arranged by size:
1553 1164 926 901 610 262 222 197
139
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2747 3413 513
Size: 552 939 743 666 3074
Fragments arranged by size:
3074 939 743 666 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2996 3169 4001 4018 4171 4356 4801
Size: 1255 1416 173 832 17 153 185 445
Cuts at: 4801 325
Size: 1498
Fragments arranged by size:
1498 1416 1255 832 445 185 173 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 2082 1943
Size: 139 5835
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 2404 2841 4103 2404
Size: 437 1262 4275
Fragments arranged by size:
4275 1262 437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI