(Circular) (Six-base) MAPSORT of: pFN21AE0758.seq Check: 8424 from: 1 to: 6001 pFN21AE0758 6001 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2713 2797 2965 3217 3271 4064 5448 5716 Size: 1081 84 168 252 54 793 1384 268 Cuts at: 5716 1632 Size: 1917 Fragments arranged by size: 1917 1384 1081 793 268 252 168 84 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 2755 4900 5877 1203 Size: 1552 2145 977 1327 Fragments arranged by size: 2145 1552 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AlwNI CAG_nnn'CTG Cuts at: 3744 5316 3744 Size: 1572 4429 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 2595 2679 2763 3183 3310 3536 3688 5797 Size: 1102 84 84 420 127 226 152 2109 Cuts at: 5797 1493 Size: 1697 Fragments arranged by size: 2109 1697 1102 420 226 152 127 84 84 AseI AT'TA_AT Cuts at: 160 1032 2394 4555 160 Size: 872 1362 2161 1606 Fragments arranged by size: 2161 1606 1362 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 4777 618 Size: 325 217 627 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3320 1391 Size: 1929 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2423 928 Size: 971 524 4506 Fragments arranged by size: 4506 971 524 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4192 4226 1903 Size: 34 50 34 2171 34 3678 Fragments arranged by size: 3678 2171 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4612 136 Size: 107 122 71 631 605 2940 1525 Fragments arranged by size: 2940 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 4062 5218 1790 Size: 2272 1156 2573 Fragments arranged by size: 2573 2272 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2065 3732 4690 Size: 416 53 83 399 24 648 1667 958 Cuts at: 4690 442 Size: 1753 Fragments arranged by size: 1753 1667 958 648 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 3045 3129 3213 4661 1239 Size: 791 1015 84 84 1448 2579 Fragments arranged by size: 2579 1448 1015 791 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2035 4664 882 Size: 617 536 2629 2219 Fragments arranged by size: 2629 2219 617 536 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3808 4190 4825 275 Size: 53 83 186 936 2275 382 635 1451 Fragments arranged by size: 2275 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5776 Fragments arranged by size: 5776 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6001 BseYI C'CCAG_C Cuts at: 5204 5700 5204 Size: 496 5505 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4062 4147 5218 4062 Size: 85 1071 4845 Fragments arranged by size: 4845 1071 85 BsmI GAATG_Cn' Cuts at: 3131 3512 3605 3131 Size: 381 93 5527 Fragments arranged by size: 5527 381 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3320 4062 4147 5218 1391 Size: 399 1530 742 85 1071 2174 Fragments arranged by size: 2174 1530 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3356 844 Size: 2512 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3298 4496 4670 65 Size: 1183 2050 1198 174 1396 Fragments arranged by size: 2050 1396 1198 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4645 4832 1700 Size: 110 2835 187 2869 Fragments arranged by size: 2869 2835 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6001 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2825 2825 Size: 6001 BstYI r'GATC_y Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996 Size: 1359 367 385 17 1452 86 12 346 Cuts at: 5996 1972 Size: 1977 Fragments arranged by size: 1977 1452 1359 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2915 2915 Size: 6001 BtgI C'CryG_G Cuts at: 513 1065 2093 513 Size: 552 1028 4421 Fragments arranged by size: 4421 1028 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2727 487 Size: 683 56 1501 3761 Fragments arranged by size: 3761 1501 683 56 BtsI GCAGTG_nn' Cuts at: 3259 3517 4311 4331 3259 Size: 258 794 20 4929 Fragments arranged by size: 4929 794 258 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2866 2901 529 Size: 35 2302 35 3629 Fragments arranged by size: 3629 2302 35 35 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2427 5008 809 Size: 507 1111 2581 1802 Fragments arranged by size: 2581 1802 1111 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3448 4337 5966 Size: 54 1425 50 275 18 1618 889 1629 Cuts at: 5966 8 Size: 43 Fragments arranged by size: 1629 1618 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 3931 1353 Size: 782 1796 3423 Fragments arranged by size: 3423 1796 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2713 2797 2965 3045 3129 3213 Size: 393 398 683 84 168 80 84 84 Cuts at: 3213 3217 3271 4064 4661 5448 5716 1239 Size: 4 54 793 597 787 268 1524 Fragments arranged by size: 1524 793 787 683 597 398 393 268 168 84 84 84 80 54 4 EcoRI G'AATT_C Cuts at: 2595 2679 2763 3183 3310 3688 2595 Size: 84 84 420 127 378 4908 Fragments arranged by size: 4908 420 378 127 84 84 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 5148 1678 Size: 3470 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3064 3096 4642 4674 4716 Size: 32 135 32 1492 32 1546 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 1546 1492 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2055 5115 5299 1305 Size: 750 3060 184 2007 Fragments arranged by size: 3060 2007 750 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4179 Fragments arranged by size: 4179 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2688 2940 3040 3959 4318 4477 518 Size: 648 1522 252 100 919 359 159 2042 Fragments arranged by size: 2042 1522 919 648 359 252 159 100 MspA1I CmG'CkG Cuts at: 1606 3237 3417 4094 5242 5487 1606 Size: 1631 180 677 1148 245 2120 Fragments arranged by size: 2120 1631 1148 677 245 180 NcoI C'CATG_G Cuts at: 513 1065 2093 513 Size: 552 1028 4421 Fragments arranged by size: 4421 1028 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2245 2759 2877 3359 4904 1132 Size: 97 1016 514 118 482 1545 2229 Fragments arranged by size: 2229 1545 1016 514 482 118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 2755 4900 2755 Size: 2145 3856 PflMI CCAn_nnn'nTGG Cuts at: 1108 2308 1108 Size: 1200 4801 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2015 2047 4070 4102 5583 5615 2015 Size: 32 2023 32 1481 32 2401 Fragments arranged by size: 2401 2023 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 3353 830 Size: 2523 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 3237 3237 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SapI GCTCTTCn'nnn_ Cuts at: 2135 2135 Size: 6001 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SexAI A'CCwGG_T Cuts at: 2025 2025 Size: 6001 SfcI C'TryA_G Cuts at: 826 1045 3238 3349 4484 5165 5356 826 Size: 219 2193 111 1135 681 191 1471 Fragments arranged by size: 2193 1471 1135 681 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4105 5006 5268 820 Size: 197 926 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 2545 3925 5 Size: 47 2493 1380 2081 Fragments arranged by size: 2493 2081 1380 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2093 2253 3440 513 Size: 552 1028 160 1187 3074 Fragments arranged by size: 3074 1187 1028 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4028 4045 4198 4383 4828 325 Size: 1255 2448 17 153 185 445 1498 Fragments arranged by size: 2448 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2990 4247 5959 Size: 274 80 33 51 1443 1013 1257 1712 Cuts at: 5959 96 Size: 138 Fragments arranged by size: 1712 1443 1257 1013 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 2345 2615 2726 2810 4130 2345 Size: 270 111 84 1320 4216 Fragments arranged by size: 4216 1320 270 111 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI