Restriction map for
pFN21AE1014
(Circular) (Six-base) MAPSORT of: pFN21AE1014.seq Check: 3787 from: 1 to: 5611
pFN21AE1014 5611 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2855 2855
Size: 5611
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2954 1937
Size: 1017 4594
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2026 2145 2401 3674 5058 5326 1632
Size: 394 119 256 1273 1384 268 1917
Fragments arranged by size:
1917 1384 1273 394 268 256 119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 2474 2508 4510 5487 1203
Size: 1271 34 2002 977 1327
Fragments arranged by size:
2002 1327 1271 977 34
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2554 2680 3354 4926 2554
Size: 126 674 1572 3239
Fragments arranged by size:
3239 1572 674 126
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2920 3146 3298 5407 1493
Size: 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2936 1870
Size: 73 993 4545
Fragments arranged by size:
4545 993 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2867 2932 4387 618
Size: 325 217 627 1080 65 1455 1842
Fragments arranged by size:
1842 1455 1080 627 325 217 65
BanII G_rGCy'C
Cuts at: 1391 2109 2257 2466 2898 2930 1391
Size: 718 148 209 432 32 4072
Fragments arranged by size:
4072 718 432 209 148 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 928
Size: 971 664 3976
Fragments arranged by size:
3976 971 664
BbvCI CC'TCA_GC
Cuts at: 2612 2612
Size: 5611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3802 3836 1903
Size: 34 50 34 1781 34 3678
Fragments arranged by size:
3678 1781 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 2108 3028 2108
Size: 920 4691
Bme1580I G_kGCm'C
Cuts at: 1790 2427 3672 4828 1790
Size: 637 1245 1156 2573
Fragments arranged by size:
2573 1245 1156 637
BmgBI CAC'GTC
Cuts at: 1318 2101 2599 1318
Size: 783 498 4330
Fragments arranged by size:
4330 783 498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4271 1239
Size: 3032 2579
Bpu10I CC'TnA_GC
Cuts at: 2612 2612
Size: 5611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2158 2475 2772 3402 493
Size: 711 954 317 297 630 2702
Fragments arranged by size:
2702 954 711 630 317 297
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2244 2884 4044 4442 4716 4863 5274 2244
Size: 640 1160 398 274 147 411 2581
Fragments arranged by size:
2581 1160 640 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 1178
Size: 30 165 30 1032 30 4324
Fragments arranged by size:
4324 1032 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5611
BseYI C'CCAG_C
Cuts at: 2011 2630 2826 4814 5310 2011
Size: 619 196 1988 496 2312
Fragments arranged by size:
2312 1988 619 496 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2854 1163
Size: 573 1118 3920
Fragments arranged by size:
3920 1118 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2271 3061 3822 4850 1540
Size: 275 186 270 790 761 1028 2301
Fragments arranged by size:
2301 1028 790 761 275 270 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2466 2898 3672 3757 4828 2109
Size: 357 432 774 85 1071 2892
Fragments arranged by size:
2892 1071 774 432 357 85
BsmI GAATG_Cn'
Cuts at: 3122 3215 3122
Size: 93 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2257 2427 2466 2898 2930 3672
Size: 399 319 148 170 39 432 32 742
Cuts at: 3672 3757 4828 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 319 170 148
85 39 32
BspEI T'CCGG_A
Cuts at: 2884 5274 2884
Size: 2390 3221
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 2966 844
Size: 2011 111 3489
Fragments arranged by size:
3489 2011 111
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2266 2270 2750 4255 4442 1700
Size: 110 456 4 480 1505 187 2869
Fragments arranged by size:
2869 1505 480 456 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 2477 96
Size: 2381 3230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5611
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 2191 1184
Size: 1007 4604
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606
Size: 969 367 385 17 1452 86 12 346
Cuts at: 5606 1972
Size: 1977
Fragments arranged by size:
1977 1452 969 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2187 513
Size: 552 1122 3937
Fragments arranged by size:
3937 1122 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2099 487
Size: 683 56 873 3999
Fragments arranged by size:
3999 873 683 56
BtsI GCAGTG_nn'
Cuts at: 2338 2396 3127 3921 3941 2338
Size: 58 731 794 20 4008
Fragments arranged by size:
4008 794 731 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2145 2915 2145
Size: 770 4841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2766 2817
Size: 54 1425 50 275 18 438 498 51
Cuts at: 2817 2902 3058 3947 5576 8
Size: 85 156 889 1629 43
Fragments arranged by size:
1629 1425 889 498 438 275 156 85
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2268 3058 1537
Size: 275 456 790 4090
Fragments arranged by size:
4090 790 456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2045 3541 1353
Size: 683 9 1496 3423
Fragments arranged by size:
3423 1496 683 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 2464 2896 2107
Size: 357 432 4822
Fragments arranged by size:
4822 432 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2145 2401 3674 4271 5058 5326
Size: 393 394 119 256 1273 597 787 268
Cuts at: 5326 1239
Size: 1524
Fragments arranged by size:
1524 1273 787 597 394 393 268 256
119
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 2439 4758 1678
Size: 761 2319 2531
Fragments arranged by size:
2531 2319 761
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2712 2744 4252 4284 4326
Size: 32 135 32 1140 32 1508 32 42
Cuts at: 4326 4358 1373
Size: 32 2626
Fragments arranged by size:
2626 1508 1140 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2875 4725 4909 1305
Size: 1570 1850 184 2007
Fragments arranged by size:
2007 1850 1570 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 2904 10
Size: 54 1425 343 987 85 2717
Fragments arranged by size:
2717 1425 987 343 85 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2859 3569 3928 4087 518
Size: 648 1693 710 359 159 2042
Fragments arranged by size:
2042 1693 710 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2112 3027 3704 4852 5097 1606
Size: 506 915 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 506 245
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 5611
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5059
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2278 2405 2584 2969 4514 1132
Size: 97 1049 127 179 385 1545 2229
Fragments arranged by size:
2229 1545 1049 385 179 127 97
PasI CC'CwG_GG
Cuts at: 1348 2362 1348
Size: 1014 4597
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 2826 1108
Size: 1254 464 3893
Fragments arranged by size:
3893 1254 464
PfoI T'CCnGG_A
Cuts at: 2020 2370 2020
Size: 350 5261
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PmlI CAC'GTG
Cuts at: 2475 2772 2475
Size: 297 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2559 2673 2963 830
Size: 1729 114 290 3478
Fragments arranged by size:
3478 1729 290 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
PvuII CAG'CTG
Cuts at: 2112 2112
Size: 5611
SacI G_AGCT'C
Cuts at: 2109 2466 2898 2109
Size: 357 432 4822
Fragments arranged by size:
4822 432 357
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SapI GCTCTTCn'nnn_
Cuts at: 2036 2045 2036
Size: 9 5602
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2555 2669 2959 4094 4775 4966 826
Size: 219 1510 114 290 1135 681 191 1471
Fragments arranged by size:
1510 1471 1135 681 290 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3715 4616 4878 820
Size: 197 926 1772 901 262 1553
Fragments arranged by size:
1772 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2158 3402 493
Size: 1665 1244 2702
Fragments arranged by size:
2702 1665 1244
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2278 2969 1132
Size: 1146 691 3774
Fragments arranged by size:
3774 1146 691
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5611
StyI C'CwwG_G
Cuts at: 513 1065 2220 3050 513
Size: 552 1155 830 3074
Fragments arranged by size:
3074 1155 830 552
SwaI ATTT'AAAT
Cuts at: 2145 2145
Size: 5611
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 2856 3638 3655 3808 3993 4438
Size: 1255 1238 38 782 17 153 185 445
Cuts at: 4438 325
Size: 1498
Fragments arranged by size:
1498 1255 1238 782 445 185 153 38
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2477 2511 3857
Size: 274 80 33 51 1443 500 34 1346
Cuts at: 3857 5569 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1346 500 274 138 80 51
34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 2722 2754 5571 5603 2435
Size: 32 255 32 2817 32 2443
Fragments arranged by size:
2817 2443 255 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2567 2600 1316
Size: 1251 33 4327
Fragments arranged by size:
4327 1251 33
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5611
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 3740 3740
Size: 5611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI XcmI