(Circular) (Six-base) MAPSORT of: pFN21AE0754.seq Check: 3954 from: 1 to: 5995 pFN21AE0754 5995 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 2627 4058 5442 5710 1632 Size: 535 460 1431 1384 268 1917 Fragments arranged by size: 1917 1431 1384 535 460 268 AfeI AGC'GCT Cuts at: 2377 2377 Size: 5995 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 2588 4894 5871 1203 Size: 1385 2306 977 1327 Fragments arranged by size: 2306 1385 1327 977 AgeI A'CCGG_T Cuts at: 2162 4826 2162 Size: 2664 3331 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2325 1184 Size: 1141 4854 AlwNI CAG_nnn'CTG Cuts at: 3023 3738 5310 3023 Size: 715 1572 3708 Fragments arranged by size: 3708 1572 715 ApaI G_GGCC'C Cuts at: 2800 2800 Size: 5995 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 2406 2437 2517 2575 2712 3104 3118 3304 Size: 913 31 80 58 137 392 14 186 Cuts at: 3304 3530 3682 5791 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 913 392 226 186 152 137 80 58 31 14 AseI AT'TA_AT Cuts at: 160 1032 2736 4549 160 Size: 872 1704 1813 1606 Fragments arranged by size: 1813 1704 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3320 1870 Size: 73 1377 4545 Fragments arranged by size: 4545 1377 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 3316 4771 618 Size: 325 217 627 372 1157 1455 1842 Fragments arranged by size: 1842 1455 1157 627 372 325 217 BanII G_rGCy'C Cuts at: 1391 2199 2800 3314 1391 Size: 808 601 514 4072 Fragments arranged by size: 4072 808 601 514 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 2496 928 Size: 971 204 393 4427 Fragments arranged by size: 4427 971 393 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 2722 1548 Size: 1174 4821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 2800 4056 5212 1790 Size: 1010 1256 1156 2573 Fragments arranged by size: 2573 1256 1156 1010 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2849 2849 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 4655 1239 Size: 974 2442 2579 Fragments arranged by size: 2579 2442 974 Bpu10I CC'TnA_GC Cuts at: 3153 3153 Size: 5995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4120 4985 5283 4120 Size: 865 298 4832 Fragments arranged by size: 4832 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2837 4658 882 Size: 617 1338 1821 2219 Fragments arranged by size: 2219 1821 1338 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 3786 493 Size: 711 1014 1568 2702 Fragments arranged by size: 2702 1568 1014 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2174 3268 3802 4184 Size: 53 83 186 936 641 1094 534 382 Cuts at: 4184 4819 275 Size: 635 1451 Fragments arranged by size: 1451 1094 936 641 635 534 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2162 4428 4826 5100 5247 5658 2162 Size: 2266 398 274 147 411 2499 Fragments arranged by size: 2499 2266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5770 Fragments arranged by size: 5770 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2774 1186 Size: 1090 498 4407 Fragments arranged by size: 4407 1090 498 BseYI C'CCAG_C Cuts at: 5198 5694 5198 Size: 496 5499 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2167 3445 4206 5234 1540 Size: 275 186 166 1278 761 1028 2301 Fragments arranged by size: 2301 1278 1028 761 275 186 166 BsiHKAI G_wGCw'C Cuts at: 3006 4056 4141 5212 3006 Size: 1050 85 1071 3789 Fragments arranged by size: 3789 1071 1050 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2800 3006 3314 4056 4141 5212 Size: 399 409 601 206 308 742 85 1071 Cuts at: 5212 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 601 409 399 308 206 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2317 3350 844 Size: 1473 1033 3489 Fragments arranged by size: 3489 1473 1033 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4490 4664 65 Size: 1183 3242 174 1396 Fragments arranged by size: 3242 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 4639 4826 1700 Size: 110 352 2477 187 2869 Fragments arranged by size: 2869 2477 352 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2692 2011 Size: 681 5314 BstYI r'GATC_y Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990 Size: 1353 367 385 17 1452 86 12 346 Cuts at: 5990 1972 Size: 1977 Fragments arranged by size: 1977 1452 1353 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 2620 3109 3299 2620 Size: 489 190 5316 Fragments arranged by size: 5316 489 190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2498 5002 809 Size: 507 1182 2504 1802 Fragments arranged by size: 2504 1802 1182 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2946 3442 4331 Size: 54 1425 50 275 18 1116 496 889 Cuts at: 4331 5960 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1116 889 496 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2186 2532 3925 1353 Size: 674 159 346 1393 3423 Fragments arranged by size: 3423 1393 674 346 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4582 4956 5102 387 Size: 1064 138 490 2503 374 146 1280 Fragments arranged by size: 2503 1280 1064 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2213 2627 4058 4655 5442 5710 Size: 393 535 46 414 1431 597 787 268 Cuts at: 5710 1239 Size: 1524 Fragments arranged by size: 1524 1431 787 597 535 414 393 268 46 EcoO109I rG'GnC_Cy Cuts at: 2796 2796 Size: 5995 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2379 5142 1678 Size: 701 2763 2531 Fragments arranged by size: 2763 2531 701 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2834 2849 2854 2866 Size: 32 135 32 1250 12 15 5 12 Cuts at: 2866 2881 4636 4668 4710 4742 1373 Size: 15 1755 32 42 32 2626 Fragments arranged by size: 2626 1755 1250 135 42 32 32 32 32 15 15 12 12 5 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 3202 748 Size: 2454 3541 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5109 5293 1305 Size: 3804 184 2007 Fragments arranged by size: 3804 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2948 3953 4312 4471 518 Size: 648 1782 1005 359 159 2042 Fragments arranged by size: 2042 1782 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3411 4088 5236 5481 1606 Size: 1805 677 1148 245 2120 Fragments arranged by size: 2120 1805 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2592 3353 4898 1132 Size: 97 1363 761 1545 2229 Fragments arranged by size: 2229 1545 1363 761 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 2588 4894 2588 Size: 2306 3689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5995 PfoI T'CCnGG_A Cuts at: 2049 2049 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 2892 3565 2892 Size: 673 5322 PspOMI G'GGCC_C Cuts at: 2796 2796 Size: 5995 PstI C_TGCA'G Cuts at: 830 3187 3347 830 Size: 2357 160 3478 Fragments arranged by size: 3478 2357 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SexAI A'CCwGG_T Cuts at: 2446 2446 Size: 5995 SfcI C'TryA_G Cuts at: 826 1045 2101 3183 3343 4478 5159 5350 826 Size: 219 1056 1082 160 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 1056 681 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4099 5000 5262 820 Size: 197 926 2156 901 262 1553 Fragments arranged by size: 2156 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 2734 3919 5 Size: 47 2682 1185 2081 Fragments arranged by size: 2682 2081 1185 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5995 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 3434 513 Size: 552 939 6 1424 3074 Fragments arranged by size: 3074 1424 939 552 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4022 4039 4192 4377 4822 325 Size: 1255 2442 17 153 185 445 1498 Fragments arranged by size: 2442 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 4241 5953 Size: 274 80 33 51 1443 296 1968 1712 Cuts at: 5953 96 Size: 138 Fragments arranged by size: 1968 1712 1443 296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 2852 3331 2852 Size: 479 5516 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2153 2807 2153 Size: 654 5341 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 2406 4124 2406 Size: 1718 4277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI