(Circular) (Six-base) MAPSORT of: pFN21AB3077.seq Check: 4897 from: 1 to: 5491 pFN21AB3077 5491 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3301 278 Size: 53 83 186 936 1765 2468 Fragments arranged by size: 2468 1765 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2769 2834 1937 Size: 832 65 4594 Fragments arranged by size: 4594 832 65 Acc65I G'GTAC_C Cuts at: 2812 2812 Size: 5491 AclI AA'CG_TT Cuts at: 1563 3618 3991 1563 Size: 2055 373 3063 Fragments arranged by size: 3063 2055 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3554 4938 5206 1632 Size: 1922 1384 268 1917 Fragments arranged by size: 1922 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5294 AflIII A'CryG_T Cuts at: 1203 2535 4390 5367 1203 Size: 1332 1855 977 1327 Fragments arranged by size: 1855 1332 1327 977 AgeI A'CCGG_T Cuts at: 4322 4322 Size: 5491 AhdI GACnn_n'nnGTC Cuts at: 4220 4220 Size: 5491 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5491 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5491 AlwNI CAG_nnn'CTG Cuts at: 3234 4806 3234 Size: 1572 3919 ApaLI G'TGCA_C Cuts at: 2478 3548 4704 2478 Size: 1070 1156 3265 Fragments arranged by size: 3265 1156 1070 ApoI r'AATT_y Cuts at: 1493 2800 3026 3178 5287 1493 Size: 1307 226 152 2109 1697 Fragments arranged by size: 2109 1697 1307 226 152 AseI AT'TA_AT Cuts at: 160 1032 4045 160 Size: 872 3013 1606 Fragments arranged by size: 3013 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5491 AvaI C'yCGr_G Cuts at: 1870 1943 2816 1870 Size: 73 873 4545 Fragments arranged by size: 4545 873 73 BamHI G'GATC_C Cuts at: 2821 3188 2821 Size: 367 5124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2242 2812 4267 618 Size: 325 217 627 455 570 1455 1842 Fragments arranged by size: 1842 1455 627 570 455 325 217 BanII G_rGCy'C Cuts at: 1391 2810 1391 Size: 1419 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 928 Size: 971 116 4404 Fragments arranged by size: 4404 971 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3682 3716 1903 Size: 34 1745 34 3678 Fragments arranged by size: 3678 1745 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3383 4593 3383 Size: 1210 4281 BclI T'GATC_A Cuts at: 1548 1548 Size: 5491 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2646 4102 136 Size: 107 122 71 631 605 974 1456 1525 Fragments arranged by size: 1525 1456 974 631 605 122 107 71 BglII A'GATC_T Cuts at: 5486 5486 Size: 5491 BlpI GC'TnA_GC Cuts at: 2908 2908 Size: 5491 Bme1580I G_kGCm'C Cuts at: 1790 2482 3552 4708 1790 Size: 692 1070 1156 2573 Fragments arranged by size: 2573 1156 1070 692 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5491 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2209 3222 4180 Size: 416 53 83 399 24 792 1013 958 Cuts at: 4180 442 Size: 1753 Fragments arranged by size: 1753 1013 958 792 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2589 4151 1239 Size: 846 504 1562 2579 Fragments arranged by size: 2579 1562 846 504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3616 4481 4779 3616 Size: 865 298 4328 Fragments arranged by size: 4328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4154 882 Size: 617 2655 2219 Fragments arranged by size: 2655 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2477 3282 493 Size: 711 1273 805 2702 Fragments arranged by size: 2702 1273 805 711 BsaBI GATnn'nnATC Cuts at: 5485 5485 Size: 5491 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3298 3680 4315 275 Size: 53 83 186 936 1765 382 635 1451 Fragments arranged by size: 1765 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3924 4322 4596 4743 5154 3924 Size: 398 274 147 411 4261 Fragments arranged by size: 4261 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2616 2646 1178 Size: 30 165 30 1213 30 4023 Fragments arranged by size: 4023 1213 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5491 BseYI C'CCAG_C Cuts at: 2145 4694 5190 2145 Size: 2549 496 2446 Fragments arranged by size: 2549 2446 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4918 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2941 3702 4730 1540 Size: 275 186 940 761 1028 2301 Fragments arranged by size: 2301 1028 940 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2482 3552 3637 4708 2482 Size: 1070 85 1071 3265 Fragments arranged by size: 3265 1071 1070 85 BsmI GAATG_Cn' Cuts at: 3002 3095 3002 Size: 93 5398 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2482 2810 3552 3637 4708 1391 Size: 399 692 328 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 399 328 85 BspEI T'CCGG_A Cuts at: 5154 5154 Size: 5491 BspHI T'CATG_A Cuts at: 2487 3380 5110 2487 Size: 893 1730 2868 Fragments arranged by size: 2868 1730 893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2846 844 Size: 2002 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3378 1394 Size: 429 1555 3507 Fragments arranged by size: 3507 1555 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3986 4160 65 Size: 1183 2738 174 1396 Fragments arranged by size: 2738 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4135 4322 1700 Size: 110 2325 187 2869 Fragments arranged by size: 2869 2325 187 110 BsrGI T'GTAC_A Cuts at: 96 2532 96 Size: 2436 3055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5491 BssSI C'ACGA_G Cuts at: 3551 4563 3551 Size: 1012 4479 BstAPI GCAn_nnn'nTGC Cuts at: 794 2060 794 Size: 1266 4225 BstBI TT'CG_AA Cuts at: 4295 4295 Size: 5491 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5491 BstYI r'GATC_y Cuts at: 1972 2821 3188 3573 3590 5042 5128 5140 5486 Size: 849 367 385 17 1452 86 12 346 Cuts at: 5486 1972 Size: 1977 Fragments arranged by size: 1977 1452 849 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4939 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2391 487 Size: 683 56 1165 3587 Fragments arranged by size: 3587 1165 683 56 BtsI GCAGTG_nn' Cuts at: 2125 2418 3007 3801 3821 2125 Size: 293 589 794 20 3795 Fragments arranged by size: 3795 794 589 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5491 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5456 DraI TTT'AAA Cuts at: 2795 2795 Size: 5491 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4498 809 Size: 507 3182 1802 Fragments arranged by size: 3182 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2938 3827 Size: 54 1425 50 275 18 202 906 889 Cuts at: 3827 5456 8 Size: 1629 43 Fragments arranged by size: 1629 1425 906 889 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2938 1537 Size: 275 1126 4090 Fragments arranged by size: 4090 1126 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 3421 1353 Size: 731 1337 3423 Fragments arranged by size: 3423 1337 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2170 4078 4452 4598 387 Size: 1064 138 581 1908 374 146 1280 Fragments arranged by size: 1908 1280 1064 581 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2589 3554 4151 4938 5206 1239 Size: 393 453 504 965 597 787 268 1524 Fragments arranged by size: 1524 965 787 597 504 453 393 268 EcoRI G'AATT_C Cuts at: 2800 3178 2800 Size: 378 5113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2588 2588 Size: 5491 FspI TGC'GCA Cuts at: 3997 3997 Size: 5491 HaeII r_GCGC'y Cuts at: 1678 4638 1678 Size: 2960 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4132 4164 4206 4238 1373 Size: 32 135 32 2560 32 42 32 2626 Fragments arranged by size: 2626 2560 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2835 3081 669 Size: 988 281 897 246 3079 Fragments arranged by size: 3079 988 897 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5491 HpaI GTT'AAC Cuts at: 3081 3081 Size: 5491 KpnI G_GTAC'C Cuts at: 2816 2816 Size: 5491 MfeI C'AATT_G Cuts at: 3090 3090 Size: 5491 MluI A'CGCG_T Cuts at: 5367 5367 Size: 5491 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4605 4789 1305 Size: 3300 184 2007 Fragments arranged by size: 3300 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2034 10 Size: 54 1425 343 202 3467 Fragments arranged by size: 3467 1425 343 202 54 MslI CAynn'nnrTG Cuts at: 518 1166 2763 3449 3808 3967 518 Size: 648 1597 686 359 159 2042 Fragments arranged by size: 2042 1597 686 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2907 3584 4732 4977 1606 Size: 432 869 677 1148 245 2120 Fragments arranged by size: 2120 1148 869 677 432 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4939 NdeI CA'TA_TG Cuts at: 387 2421 387 Size: 2034 3457 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5491 NotI GC'GGCC_GC Cuts at: 2938 2938 Size: 5491 NspI r_CATG'y Cuts at: 1132 1229 2539 2849 4394 1132 Size: 97 1310 310 1545 2229 Fragments arranged by size: 2229 1545 1310 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5491 PciI A'CATG_T Cuts at: 2535 4390 2535 Size: 1855 3636 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5491 PmeI GTTT'AAAC Cuts at: 2795 2795 Size: 5491 PmlI CAC'GTG Cuts at: 2477 2477 Size: 5491 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3560 3592 5073 5105 3560 Size: 32 1481 32 3946 Fragments arranged by size: 3946 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5491 PsiI TTA'TAA Cuts at: 2270 3061 2270 Size: 791 4700 PstI C_TGCA'G Cuts at: 830 2174 2843 830 Size: 1344 669 3478 Fragments arranged by size: 3478 1344 669 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5491 SalI G'TCGA_C Cuts at: 1936 2833 1936 Size: 897 4594 SbfI CC_TGCA'GG Cuts at: 2843 2843 Size: 5491 ScaI AGT'ACT Cuts at: 3739 5451 3739 Size: 1712 3779 SexAI A'CCwGG_T Cuts at: 2238 2238 Size: 5491 SfcI C'TryA_G Cuts at: 826 1045 2170 2839 3974 4655 4846 826 Size: 219 1125 669 1135 681 191 1471 Fragments arranged by size: 1471 1135 1125 681 669 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5491 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5491 SmaI CCC'GGG Cuts at: 1872 2818 1872 Size: 946 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3595 4496 4758 820 Size: 197 926 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3282 493 Size: 2789 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5491 SphI G_CATG'C Cuts at: 1132 2849 1132 Size: 1717 3774 SspI AAT'ATT Cuts at: 5 52 3415 5 Size: 47 3363 2081 Fragments arranged by size: 3363 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5491 StyI C'CwwG_G Cuts at: 513 1065 2930 513 Size: 552 1865 3074 Fragments arranged by size: 3074 1865 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2175 3518 3535 3688 3873 4318 325 Size: 1255 595 1343 17 153 185 445 1498 Fragments arranged by size: 1498 1343 1255 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 3737 5449 Size: 274 80 33 51 1443 555 1205 1712 Cuts at: 5449 96 Size: 138 Fragments arranged by size: 1712 1443 1205 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 5451 5483 2616 Size: 32 2803 32 2624 Fragments arranged by size: 2803 2624 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2525 1316 Size: 1209 4282 XbaI T'CTAG_A Cuts at: 2827 2827 Size: 5491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5491 XmaI C'CCGG_G Cuts at: 1870 2816 1870 Size: 946 4545 XmnI GAAnn'nnTTC Cuts at: 3620 3620 Size: 5491 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3299 276 Size: 53 83 186 936 1765 2468 Fragments arranged by size: 2468 1765 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI