(Circular) (Six-base) MAPSORT of: pFN21AA0869.seq Check: 8543 from: 1 to: 8044 pFN21AA0869 8044 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3238 3238 Size: 8044 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5343 5854 278 Size: 53 83 186 936 3807 511 2468 Fragments arranged by size: 3807 2468 936 511 186 83 53 AccI GT'mk_AC Cuts at: 1937 2070 4334 5387 1937 Size: 133 2264 1053 4594 Fragments arranged by size: 4594 2264 1053 133 Acc65I G'GTAC_C Cuts at: 4647 5365 4647 Size: 718 7326 AclI AA'CG_TT Cuts at: 1563 6171 6544 1563 Size: 4608 373 3063 Fragments arranged by size: 4608 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 2546 2609 2978 3077 3363 3918 4022 Size: 680 234 63 369 99 286 555 104 Cuts at: 4022 5060 5171 6107 7491 7759 1632 Size: 1038 111 936 1384 268 1917 Fragments arranged by size: 1917 1384 1038 936 680 555 369 286 268 234 111 104 99 63 AflII C'TTAA_G Cuts at: 820 1017 4234 820 Size: 197 3217 4630 Fragments arranged by size: 4630 3217 197 AflIII A'CryG_T Cuts at: 1203 4818 6943 7920 1203 Size: 3615 2125 977 1327 Fragments arranged by size: 3615 2125 1327 977 AgeI A'CCGG_T Cuts at: 6875 6875 Size: 8044 AhdI GACnn_n'nnGTC Cuts at: 2764 2782 4444 4904 6773 2764 Size: 18 1662 460 1869 4035 Fragments arranged by size: 4035 1869 1662 460 18 AleI CACnn'nnGTG Cuts at: 1166 4175 4709 5178 1166 Size: 3009 534 469 4032 Fragments arranged by size: 4032 3009 534 469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2415 1184 Size: 1231 6813 AlwNI CAG_nnn'CTG Cuts at: 2365 2395 2587 2836 3301 3565 3887 3925 3941 Size: 30 192 249 465 264 322 38 16 Cuts at: 3941 4427 4516 5787 7359 2365 Size: 486 89 1271 1572 3050 Fragments arranged by size: 3050 1572 1271 486 465 322 264 249 192 89 38 30 16 ApaLI G'TGCA_C Cuts at: 2328 5225 6101 7257 2328 Size: 2897 876 1156 3115 Fragments arranged by size: 3115 2897 1156 876 ApoI r'AATT_y Cuts at: 1493 3987 5353 5579 5731 7840 1493 Size: 2494 1366 226 152 2109 1697 Fragments arranged by size: 2494 2109 1697 1366 226 152 AseI AT'TA_AT Cuts at: 160 1032 4037 6598 160 Size: 872 3005 2561 1606 Fragments arranged by size: 3005 2561 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8044 AvaI C'yCGr_G Cuts at: 1870 1943 2256 2511 2863 3156 3487 5369 1870 Size: 73 313 255 352 293 331 1882 4545 Fragments arranged by size: 4545 1882 352 331 313 293 255 73 BamHI G'GATC_C Cuts at: 2251 3323 5374 5741 2251 Size: 1072 2051 367 4554 Fragments arranged by size: 4554 2051 1072 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3581 4289 4647 4736 5365 Size: 325 217 627 1794 708 358 89 629 Cuts at: 5365 6820 618 Size: 1455 1842 Fragments arranged by size: 1842 1794 1455 708 629 627 358 325 217 89 BanII G_rGCy'C Cuts at: 1391 2426 2849 3347 4392 5049 5363 1391 Size: 1035 423 498 1045 657 314 4072 Fragments arranged by size: 4072 1045 1035 657 498 423 314 BbeI G_GCGC'C Cuts at: 4293 4293 Size: 8044 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3714 4235 4527 928 Size: 971 1815 521 292 4445 Fragments arranged by size: 4445 1815 971 521 292 BbvCI CC'TCA_GC Cuts at: 5135 5135 Size: 8044 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6235 6269 1903 Size: 34 50 34 4214 34 3678 Fragments arranged by size: 4214 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4210 5936 7146 4210 Size: 1726 1210 5108 Fragments arranged by size: 5108 1726 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8044 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 4287 6655 Size: 107 122 71 631 605 646 1969 2368 Cuts at: 6655 136 Size: 1525 Fragments arranged by size: 2368 1969 1525 646 631 605 122 107 71 BglII A'GATC_T Cuts at: 2340 2713 5028 8039 2340 Size: 373 2315 3011 2345 Fragments arranged by size: 3011 2345 2315 373 BlpI GC'TnA_GC Cuts at: 5461 5461 Size: 8044 Bme1580I G_kGCm'C Cuts at: 1790 2332 4739 5229 6105 7261 1790 Size: 542 2407 490 876 1156 2573 Fragments arranged by size: 2573 2407 1156 876 542 490 BmgBI CAC'GTC Cuts at: 1318 3333 1318 Size: 2015 6029 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3935 5775 6733 Size: 416 53 83 399 24 2518 1840 958 Cuts at: 6733 442 Size: 1753 Fragments arranged by size: 2518 1840 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8044 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3744 4353 4645 3744 Size: 609 292 7143 Fragments arranged by size: 7143 609 292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2531 2822 3134 3251 3482 3797 3909 Size: 1148 144 291 312 117 231 315 112 Cuts at: 3909 4499 4549 4913 4997 5165 6704 1239 Size: 590 50 364 84 168 1539 2579 Fragments arranged by size: 2579 1539 1148 590 364 315 312 291 231 168 144 117 112 84 50 Bpu10I CC'TnA_GC Cuts at: 2619 2646 5135 2619 Size: 27 2489 5528 Fragments arranged by size: 5528 2489 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2677 3268 4376 5033 6169 7034 7332 2677 Size: 591 1108 657 1136 865 298 3389 Fragments arranged by size: 3389 1136 1108 865 657 591 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2446 2772 4535 4667 4679 6707 882 Size: 617 947 326 1763 132 12 2028 2219 Fragments arranged by size: 2219 2028 1763 947 617 326 132 12 BsaAI yAC'GTr Cuts at: 493 1204 4819 5835 493 Size: 711 3615 1016 2702 Fragments arranged by size: 3615 2702 1016 711 BsaBI GATnn'nnATC Cuts at: 3032 5315 8038 3032 Size: 2283 2723 3038 Fragments arranged by size: 3038 2723 2283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4161 4290 5340 5851 Size: 53 83 186 936 2628 129 1050 511 Cuts at: 5851 6233 6868 275 Size: 382 635 1451 Fragments arranged by size: 2628 1451 1050 936 635 511 382 186 129 83 53 BsaWI w'CCGG_w Cuts at: 4143 4725 4908 6477 6875 7149 7296 7707 4143 Size: 582 183 1569 398 274 147 411 4480 Fragments arranged by size: 4480 1569 582 411 398 274 183 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4828 4858 1178 Size: 30 165 30 3425 30 4364 Fragments arranged by size: 4364 3425 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 3062 3361 3616 4553 4589 4592 4595 Size: 871 1005 299 255 937 36 3 3 Cuts at: 4595 4604 4607 4610 1186 Size: 9 3 3 4620 Fragments arranged by size: 4620 1005 937 871 299 255 36 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2037 2419 3267 3805 4651 5320 7247 7743 2037 Size: 382 848 538 846 669 1927 496 2338 Fragments arranged by size: 2338 1927 848 846 669 538 496 382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3245 3938 1163 Size: 573 1509 693 5269 Fragments arranged by size: 5269 1509 693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 5494 6255 7283 1540 Size: 275 186 14 3479 761 1028 2301 Fragments arranged by size: 3479 2301 1028 761 275 186 14 BsiHKAI G_wGCw'C Cuts at: 2332 2849 4392 4772 5049 5175 5229 6105 6190 Size: 517 1543 380 277 126 54 876 85 Cuts at: 6190 7261 2332 Size: 1071 3115 Fragments arranged by size: 3115 1543 1071 876 517 380 277 126 85 54 BsmI GAATG_Cn' Cuts at: 5555 5648 5555 Size: 93 7951 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2426 2849 3347 4392 4739 4772 Size: 399 542 94 423 498 1045 347 33 Cuts at: 4772 5049 5175 5229 5363 6105 6190 7261 1391 Size: 277 126 54 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 1045 742 542 498 423 399 347 277 134 126 94 85 54 33 BspEI T'CCGG_A Cuts at: 4143 4908 7707 4143 Size: 765 2799 4480 Fragments arranged by size: 4480 2799 765 BspHI T'CATG_A Cuts at: 5933 7663 5933 Size: 1730 6314 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3153 3238 4142 4995 5399 844 Size: 2309 85 904 853 404 3489 Fragments arranged by size: 3489 2309 904 853 404 85 BsrBI CCG'CTC Cuts at: 1394 1823 2137 4782 5168 5931 1394 Size: 429 314 2645 386 763 3507 Fragments arranged by size: 3507 2645 763 429 386 314 BsrDI GCAATG_nn' Cuts at: 65 1248 6539 6713 65 Size: 1183 5291 174 1396 Fragments arranged by size: 5291 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3021 3473 3771 4317 4824 6688 6875 Size: 110 1211 452 298 546 507 1864 187 Cuts at: 6875 1700 Size: 2869 Fragments arranged by size: 2869 1864 1211 546 507 452 298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8044 BssHII G'CGCG_C Cuts at: 1925 2048 1925 Size: 123 7921 BssSI C'ACGA_G Cuts at: 2552 3509 6104 7116 2552 Size: 957 2595 1012 3480 Fragments arranged by size: 3480 2595 1012 957 BstAPI GCAn_nnn'nTGC Cuts at: 794 2557 3565 3977 4134 794 Size: 1763 1008 412 157 4704 Fragments arranged by size: 4704 1763 1008 412 157 BstBI TT'CG_AA Cuts at: 4882 6848 4882 Size: 1966 6078 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8044 BstXI CCAn_nnnn'nTGG Cuts at: 3836 3836 Size: 8044 BstYI r'GATC_y Cuts at: 1972 2251 2340 2478 2713 2961 3323 5028 5374 Size: 279 89 138 235 248 362 1705 346 Cuts at: 5374 5741 6126 6143 7595 7681 7693 8039 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1705 1452 385 367 362 346 346 279 248 235 138 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3307 4420 4719 3307 Size: 1113 299 6632 Fragments arranged by size: 6632 1113 299 BtgI C'CryG_G Cuts at: 513 1065 2114 5077 513 Size: 552 1049 2963 3480 Fragments arranged by size: 3480 2963 1049 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7305 Fragments arranged by size: 7305 683 56 BtsI GCAGTG_nn' Cuts at: 2499 3978 4476 5560 6354 6374 2499 Size: 1479 498 1084 794 20 4169 Fragments arranged by size: 4169 1479 1084 794 498 20 ClaI AT'CG_AT Cuts at: 1554 2598 1554 Size: 1044 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2227 2262 2554 2589 5085 5120 529 Size: 35 1663 35 292 35 2496 35 3453 Fragments arranged by size: 3453 2496 1663 292 35 35 35 35 DraI TTT'AAA Cuts at: 5348 5348 Size: 8044 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2066 7051 809 Size: 507 750 4985 1802 Fragments arranged by size: 4985 1802 750 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2970 3522 Size: 54 1425 50 275 18 182 958 552 Cuts at: 3522 3550 5491 6380 8009 8 Size: 28 1941 889 1629 43 Fragments arranged by size: 1941 1629 1425 958 889 552 275 182 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2012 5491 1537 Size: 275 200 3479 4090 Fragments arranged by size: 4090 3479 275 200 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2522 2925 4269 4478 4566 4599 5974 Size: 825 344 403 1344 209 88 33 1375 Cuts at: 5974 1353 Size: 3423 Fragments arranged by size: 3423 1375 1344 825 403 344 209 88 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4322 5114 6631 7005 7151 387 Size: 1064 138 2733 792 1517 374 146 1280 Fragments arranged by size: 2733 1517 1280 1064 792 374 146 138 EcoICRI GAG'CTC Cuts at: 2847 4390 5047 2847 Size: 1543 657 5844 Fragments arranged by size: 5844 1543 657 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2387 2531 2546 2609 2822 2978 Size: 393 680 75 144 15 63 213 156 Cuts at: 2978 3077 3134 3251 3363 3482 3797 3909 3918 Size: 99 57 117 112 119 315 112 9 Cuts at: 3918 4022 4499 4549 4913 4997 5060 5165 5171 Size: 104 477 50 364 84 63 105 6 Cuts at: 5171 6107 6704 7491 7759 1239 Size: 936 597 787 268 1524 Fragments arranged by size: 1524 936 787 680 597 477 393 364 315 268 213 156 144 119 117 112 112 105 104 99 84 75 63 63 57 50 15 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2030 4307 4419 2030 Size: 2277 112 5655 Fragments arranged by size: 5655 2277 112 EcoO109I rG'GnC_Cy Cuts at: 3142 4424 4802 3142 Size: 1282 378 6384 Fragments arranged by size: 6384 1282 378 EcoRI G'AATT_C Cuts at: 3987 5353 5731 3987 Size: 1366 378 6300 Fragments arranged by size: 6300 1366 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 8044 FspI TGC'GCA Cuts at: 6550 6550 Size: 8044 HaeII r_GCGC'y Cuts at: 1678 2365 3179 3759 4293 7191 1678 Size: 687 814 580 534 2898 2531 Fragments arranged by size: 2898 2531 814 687 580 534 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2236 2268 2750 2768 2782 Size: 32 135 32 664 32 482 18 14 Cuts at: 2782 2800 2831 2863 4138 4170 4230 4262 4430 Size: 18 31 32 1275 32 60 32 168 Cuts at: 4430 4462 4890 4922 6685 6717 6759 6791 1373 Size: 32 428 32 1763 32 42 32 2626 Fragments arranged by size: 2626 1763 1275 664 482 428 168 135 60 42 32 32 32 32 32 32 32 32 32 32 31 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2518 4335 5388 5634 669 Size: 988 281 580 1817 1053 246 3079 Fragments arranged by size: 3079 1817 1053 988 580 281 246 HindIII A'AGCT_T Cuts at: 748 2869 2975 748 Size: 2121 106 5817 Fragments arranged by size: 5817 2121 106 HpaI GTT'AAC Cuts at: 5634 5634 Size: 8044 KasI G'GCGC_C Cuts at: 4289 4289 Size: 8044 KpnI G_GTAC'C Cuts at: 4651 5369 4651 Size: 718 7326 MfeI C'AATT_G Cuts at: 5015 5643 5015 Size: 628 7416 MluI A'CGCG_T Cuts at: 7920 7920 Size: 8044 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 4437 7158 7342 1305 Size: 880 2252 2721 184 2007 Fragments arranged by size: 2721 2252 2007 880 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2972 3524 10 Size: 54 1425 343 1140 552 4530 Fragments arranged by size: 4530 1425 1140 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3513 4175 4709 5178 6002 6361 6520 Size: 648 2347 662 534 469 824 359 159 Cuts at: 6520 518 Size: 2042 Fragments arranged by size: 2347 2042 824 662 648 534 469 359 159 MspA1I CmG'CkG Cuts at: 1606 2103 2116 2968 3112 3271 3421 3805 4048 Size: 497 13 852 144 159 150 384 243 Cuts at: 4048 4527 5059 5213 5460 6137 7285 7530 1606 Size: 479 532 154 247 677 1148 245 2120 Fragments arranged by size: 2120 1148 852 677 532 497 479 384 247 245 243 159 154 150 144 13 NaeI GCC'GGC Cuts at: 4826 4826 Size: 8044 NarI GG'CG_CC Cuts at: 4290 4290 Size: 8044 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7492 NdeI CA'TA_TG Cuts at: 387 2191 2286 387 Size: 1804 95 6145 Fragments arranged by size: 6145 1804 95 NgoMIV G'CCGG_C Cuts at: 4824 4824 Size: 8044 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8044 NotI GC'GGCC_GC Cuts at: 5491 5491 Size: 8044 NspI r_CATG'y Cuts at: 1132 1229 2402 3295 5402 6947 1132 Size: 97 1173 893 2107 1545 2229 Fragments arranged by size: 2229 2107 1545 1173 893 97 PasI CC'CwG_GG Cuts at: 1348 3094 3829 4747 1348 Size: 1746 735 918 4645 Fragments arranged by size: 4645 1746 918 735 PciI A'CATG_T Cuts at: 6943 6943 Size: 8044 PflMI CCAn_nnn'nTGG Cuts at: 1108 2103 2204 1108 Size: 995 101 6948 Fragments arranged by size: 6948 995 101 PfoI T'CCnGG_A Cuts at: 3459 3459 Size: 8044 PmeI GTTT'AAAC Cuts at: 5348 5348 Size: 8044 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2523 2546 2555 2578 6113 6145 7626 7658 2523 Size: 23 9 23 3535 32 1481 32 2909 Fragments arranged by size: 3535 2909 1481 32 32 23 23 9 PpuMI rG'GwC_Cy Cuts at: 3142 4802 3142 Size: 1660 6384 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8044 PsiI TTA'TAA Cuts at: 5614 5614 Size: 8044 PspXI vC'TCGA_Gb Cuts at: 2256 2256 Size: 8044 PstI C_TGCA'G Cuts at: 830 2562 3150 3438 3561 4992 5335 5396 830 Size: 1732 588 288 123 1431 343 61 3478 Fragments arranged by size: 3478 1732 1431 588 343 288 123 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8044 PvuII CAG'CTG Cuts at: 2103 2968 3112 3271 3421 3805 4048 4527 5059 Size: 865 144 159 150 384 243 479 532 Cuts at: 5059 5213 2103 Size: 154 4934 Fragments arranged by size: 4934 865 532 479 384 243 159 154 150 144 SacI G_AGCT'C Cuts at: 2849 4392 5049 2849 Size: 1543 657 5844 Fragments arranged by size: 5844 1543 657 SacII CC_GC'GG Cuts at: 2117 2117 Size: 8044 SalI G'TCGA_C Cuts at: 1936 4333 5386 1936 Size: 2397 1053 4594 Fragments arranged by size: 4594 2397 1053 SbfI CC_TGCA'GG Cuts at: 3150 3561 4992 5396 3150 Size: 411 1431 404 5798 Fragments arranged by size: 5798 1431 411 404 ScaI AGT'ACT Cuts at: 6292 8004 6292 Size: 1712 6332 SexAI A'CCwGG_T Cuts at: 2301 2346 2301 Size: 45 7999 SfcI C'TryA_G Cuts at: 826 1045 2558 3146 3434 3557 4988 5331 5392 Size: 219 1513 588 288 123 1431 343 61 Cuts at: 5392 6527 7208 7399 826 Size: 1135 681 191 1471 Fragments arranged by size: 1513 1471 1431 1135 681 588 343 288 219 191 123 61 SfoI GGC'GCC Cuts at: 4291 4291 Size: 8044 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8044 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8044 SmaI CCC'GGG Cuts at: 1872 2513 3158 5371 1872 Size: 641 645 2213 4545 Fragments arranged by size: 4545 2213 645 641 SmlI C'TyrA_G Cuts at: 820 1017 1943 2256 2656 3283 4234 4391 5048 Size: 197 926 313 400 627 951 157 657 Cuts at: 5048 6148 7049 7311 820 Size: 1100 901 262 1553 Fragments arranged by size: 1553 1100 951 926 901 657 627 400 313 262 197 157 SnaBI TAC'GTA Cuts at: 493 5835 493 Size: 5342 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8044 SphI G_CATG'C Cuts at: 1132 2402 3295 5402 1132 Size: 1270 893 2107 3774 Fragments arranged by size: 3774 2107 1270 893 SspI AAT'ATT Cuts at: 5 52 4035 5968 5 Size: 47 3983 1933 2081 Fragments arranged by size: 3983 2081 1933 47 StuI AGG'CCT Cuts at: 1517 2990 1517 Size: 1473 6571 StyI C'CwwG_G Cuts at: 513 1065 3696 3745 5217 5483 513 Size: 552 2631 49 1472 266 3074 Fragments arranged by size: 3074 2631 1472 552 266 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3179 3917 3965 4918 5178 6071 6088 Size: 1255 1599 738 48 953 260 893 17 Cuts at: 6088 6241 6426 6871 325 Size: 153 185 445 1498 Fragments arranged by size: 1599 1498 1255 953 893 738 445 260 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2260 6290 8002 Size: 274 80 33 51 1443 283 4030 1712 Cuts at: 8002 96 Size: 138 Fragments arranged by size: 4030 1712 1443 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4828 4860 8004 8036 4828 Size: 32 3144 32 4836 Fragments arranged by size: 4836 3144 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8044 XbaI T'CTAG_A Cuts at: 5380 5380 Size: 8044 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3247 3247 Size: 8044 XhoI C'TCGA_G Cuts at: 1943 2256 1943 Size: 313 7731 XmaI C'CCGG_G Cuts at: 1870 2511 3156 5369 1870 Size: 641 645 2213 4545 Fragments arranged by size: 4545 2213 645 641 XmnI GAAnn'nnTTC Cuts at: 6173 6173 Size: 8044 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5341 5852 276 Size: 53 83 186 936 3807 511 2468 Fragments arranged by size: 3807 2468 936 511 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI RsrII SanDI SapI SfiI SrfI SwaI