Restriction map for
pFN21AA0869
(Linear) (Six-base) MAPSORT of: KIAA0869.seq Check: 7285 from: 1 to: 3339
KIAA0869.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1233 3339
Size: 1233 2106
AatII G_ACGT'C
Cuts at: 0 3338 3339
Size: 3338 1
AccI GT'mk_AC
Cuts at: 0 65 2329 3339
Size: 65 2264 1010
Fragments arranged by size:
2264 1010 65
Acc65I G'GTAC_C
Cuts at: 0 2642 3339
Size: 2642 697
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 541 604 973 1072 1358 1913 2017
Size: 307 234 63 369 99 286 555 104
Cuts at: 2017 3055 3166 3339
Size: 1038 111 173
Fragments arranged by size:
1038 555 369 307 286 234 173 111
104 99 63
AflII C'TTAA_G
Cuts at: 0 2229 3339
Size: 2229 1110
AflIII A'CryG_T
Cuts at: 0 2813 3339
Size: 2813 526
AhdI GACnn_n'nnGTC
Cuts at: 0 759 777 2439 2899 3339
Size: 759 18 1662 460 440
Fragments arranged by size:
1662 759 460 440 18
AleI CACnn'nnGTG
Cuts at: 0 2170 2704 3173 3339
Size: 2170 534 469 166
Fragments arranged by size:
2170 534 469 166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 410 3339
Size: 410 2929
AlwNI CAG_nnn'CTG
Cuts at: 0 360 390 582 831 1296 1560 1882 1920
Size: 360 30 192 249 465 264 322 38
Cuts at: 1920 1936 2422 2511 3339
Size: 16 486 89 828
Fragments arranged by size:
828 486 465 360 322 264 249 192
89 38 30 16
ApaLI G'TGCA_C
Cuts at: 0 323 3220 3339
Size: 323 2897 119
Fragments arranged by size:
2897 323 119
ApoI r'AATT_y
Cuts at: 0 1982 3339
Size: 1982 1357
AseI AT'TA_AT
Cuts at: 0 2032 3339
Size: 2032 1307
AvaI C'yCGr_G
Cuts at: 0 251 506 858 1151 1482 3339
Size: 251 255 352 293 331 1857
Fragments arranged by size:
1857 352 331 293 255 251
BamHI G'GATC_C
Cuts at: 0 246 1318 3339
Size: 246 1072 2021
Fragments arranged by size:
2021 1072 246
BanI G'GyrC_C
Cuts at: 0 1576 2284 2642 2731 3339
Size: 1576 708 358 89 608
Fragments arranged by size:
1576 708 608 358 89
BanII G_rGCy'C
Cuts at: 0 421 844 1342 2387 3044 3339
Size: 421 423 498 1045 657 295
Fragments arranged by size:
1045 657 498 423 421 295
BbeI G_GCGC'C
Cuts at: 0 2288 3339
Size: 2288 1051
BbsI GAAGACnn'nnnn_
Cuts at: 0 1709 2230 2522 3339
Size: 1709 521 292 817
Fragments arranged by size:
1709 817 521 292
BbvCI CC'TCA_GC
Cuts at: 0 3130 3339
Size: 3130 209
BciVI GTATCCnnnnn_n'
Cuts at: 0 2205 3339
Size: 2205 1134
BglI GCCn_nnn'nGGC
Cuts at: 0 313 2282 3339
Size: 313 1969 1057
Fragments arranged by size:
1969 1057 313
BglII A'GATC_T
Cuts at: 0 335 708 3023 3339
Size: 335 373 2315 316
Fragments arranged by size:
2315 373 335 316
Bme1580I G_kGCm'C
Cuts at: 0 327 2734 3224 3339
Size: 327 2407 490 115
Fragments arranged by size:
2407 490 327 115
BmgBI CAC'GTC
Cuts at: 0 1328 3339
Size: 1328 2011
BmrI ACTGGGnnnn_n'
Cuts at: 0 1930 3339
Size: 1930 1409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1739 2348 2640 3339
Size: 1739 609 292 699
Fragments arranged by size:
1739 699 609 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 526 817 1129 1246 1477 1792 1904
Size: 382 144 291 312 117 231 315 112
Cuts at: 1904 2494 2544 2908 2992 3160 3339
Size: 590 50 364 84 168 179
Fragments arranged by size:
590 382 364 315 312 291 231 179
168 144 117 112 84 50
Bpu10I CC'TnA_GC
Cuts at: 0 614 641 3130 3339
Size: 614 27 2489 209
Fragments arranged by size:
2489 614 209 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 1263 2371 3028 3339
Size: 672 591 1108 657 311
Fragments arranged by size:
1108 672 657 591 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 441 767 2530 2662 2674 3339
Size: 441 326 1763 132 12 665
Fragments arranged by size:
1763 665 441 326 132 12
BsaAI yAC'GTr
Cuts at: 0 2814 3339
Size: 2814 525
BsaBI GATnn'nnATC
Cuts at: 0 1027 3310 3339
Size: 1027 2283 29
Fragments arranged by size:
2283 1027 29
BsaHI Gr'CG_yC
Cuts at: 0 2156 2285 3335 3339
Size: 2156 129 1050 4
Fragments arranged by size:
2156 1050 129 4
BsaWI w'CCGG_w
Cuts at: 0 2138 2720 2903 3339
Size: 2138 582 183 436
Fragments arranged by size:
2138 582 436 183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2823 2853 3339
Size: 2823 30 486
Fragments arranged by size:
2823 486 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1057 1356 1611 2548 2584 2587 2590
Size: 52 1005 299 255 937 36 3 3
Cuts at: 2590 2599 2602 2605 3339
Size: 9 3 3 734
Fragments arranged by size:
1005 937 734 299 255 52 36 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 32 414 1262 1800 2646 3315 3339
Size: 32 382 848 538 846 669 24
Fragments arranged by size:
848 846 669 538 382 32 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1240 1933 3339
Size: 1240 693 1406
Fragments arranged by size:
1406 1240 693
BsiEI CG_ry'CG
Cuts at: 0 10 3339
Size: 10 3329
BsiHKAI G_wGCw'C
Cuts at: 0 327 844 2387 2767 3044 3170 3224 3339
Size: 327 517 1543 380 277 126 54 115
Fragments arranged by size:
1543 517 380 327 277 126 115 54
Bsp1286I G_dGCh'C
Cuts at: 0 327 421 844 1342 2387 2734 2767 3044
Size: 327 94 423 498 1045 347 33 277
Cuts at: 3044 3170 3224 3339
Size: 126 54 115
Fragments arranged by size:
1045 498 423 347 327 277 126 115
94 54 33
BspEI T'CCGG_A
Cuts at: 0 2138 2903 3339
Size: 2138 765 436
Fragments arranged by size:
2138 765 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1148 1233 2137 2990 3339
Size: 1148 85 904 853 349
Fragments arranged by size:
1148 904 853 349 85
BsrBI CCG'CTC
Cuts at: 0 132 2777 3163 3339
Size: 132 2645 386 176
Fragments arranged by size:
2645 386 176 132
BsrFI r'CCGG_y
Cuts at: 0 1016 1468 1766 2312 2819 3339
Size: 1016 452 298 546 507 520
Fragments arranged by size:
1016 546 520 507 452 298
BssHII G'CGCG_C
Cuts at: 0 43 3339
Size: 43 3296
BssSI C'ACGA_G
Cuts at: 0 547 1504 3339
Size: 547 957 1835
Fragments arranged by size:
1835 957 547
BstAPI GCAn_nnn'nTGC
Cuts at: 0 552 1560 1972 2129 3339
Size: 552 1008 412 157 1210
Fragments arranged by size:
1210 1008 552 412 157
BstBI TT'CG_AA
Cuts at: 0 2877 3339
Size: 2877 462
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1831 3339
Size: 1831 1508
BstYI r'GATC_y
Cuts at: 0 246 335 473 708 956 1318 3023 3339
Size: 246 89 138 235 248 362 1705 316
Fragments arranged by size:
1705 362 316 248 246 235 138 89
Bsu36I CC'TnA_GG
Cuts at: 0 1302 2415 2714 3339
Size: 1302 1113 299 625
Fragments arranged by size:
1302 1113 625 299
BtgI C'CryG_G
Cuts at: 0 109 3072 3339
Size: 109 2963 267
Fragments arranged by size:
2963 267 109
BtsI GCAGTG_nn'
Cuts at: 0 494 1973 2471 3339
Size: 494 1479 498 868
Fragments arranged by size:
1479 868 498 494
ClaI AT'CG_AT
Cuts at: 0 593 3339
Size: 593 2746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 222 257 549 584 3080 3115 3339
Size: 222 35 292 35 2496 35 224
Fragments arranged by size:
2496 292 224 222 35 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 61 3339
Size: 61 3278
EaeI y'GGCC_r
Cuts at: 0 7 965 1517 1545 3339
Size: 7 958 552 28 1794
Fragments arranged by size:
1794 958 552 28 7
EagI C'GGCC_G
Cuts at: 0 7 3339
Size: 7 3332
EarI CTCTTCn'nnn_
Cuts at: 0 173 517 920 2264 2473 2561 2594 3339
Size: 173 344 403 1344 209 88 33 745
Fragments arranged by size:
1344 745 403 344 209 173 88 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2317 3109 3339
Size: 2317 792 230
Fragments arranged by size:
2317 792 230
EcoICRI GAG'CTC
Cuts at: 0 842 2385 3042 3339
Size: 842 1543 657 297
Fragments arranged by size:
1543 842 657 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 382 526 541 604 817 973 1072
Size: 307 75 144 15 63 213 156 99
Cuts at: 1072 1129 1246 1358 1477 1792 1904 1913 2017
Size: 57 117 112 119 315 112 9 104
Cuts at: 2017 2494 2544 2908 2992 3055 3160 3166 3339
Size: 477 50 364 84 63 105 6 173
Fragments arranged by size:
477 364 315 307 213 173 156 144
119 117 112 112 105 104 99 84
75 63 63 57 50 15 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 2302 2414 3339
Size: 25 2277 112 925
Fragments arranged by size:
2277 925 112 25
EcoO109I rG'GnC_Cy
Cuts at: 0 1137 2419 2797 3339
Size: 1137 1282 378 542
Fragments arranged by size:
1282 1137 542 378
EcoRI G'AATT_C
Cuts at: 0 1982 3339
Size: 1982 1357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 3339
Size: 759 2580
HaeII r_GCGC'y
Cuts at: 0 360 1174 1754 2288 3339
Size: 360 814 580 534 1051
Fragments arranged by size:
1051 814 580 534 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 231 263 745 763 777 795 826 858
Size: 231 32 482 18 14 18 31 32
Cuts at: 858 2133 2165 2225 2257 2425 2457 2885 2917
Size: 1275 32 60 32 168 32 428 32
Cuts at: 2917 3339
Size: 422
Fragments arranged by size:
1275 482 428 422 231 168 60 32
32 32 32 32 32 31 18 18
14
HincII GTy'rAC
Cuts at: 0 513 2330 3339
Size: 513 1817 1009
Fragments arranged by size:
1817 1009 513
HindIII A'AGCT_T
Cuts at: 0 864 970 3339
Size: 864 106 2369
Fragments arranged by size:
2369 864 106
KasI G'GCGC_C
Cuts at: 0 2284 3339
Size: 2284 1055
KpnI G_GTAC'C
Cuts at: 0 2646 3339
Size: 2646 693
MfeI C'AATT_G
Cuts at: 0 3010 3339
Size: 3010 329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 2432 3339
Size: 180 2252 907
Fragments arranged by size:
2252 907 180
MscI TGG'CCA
Cuts at: 0 967 1519 3339
Size: 967 552 1820
Fragments arranged by size:
1820 967 552
MslI CAynn'nnrTG
Cuts at: 0 1508 2170 2704 3173 3339
Size: 1508 662 534 469 166
Fragments arranged by size:
1508 662 534 469 166
MspA1I CmG'CkG
Cuts at: 0 98 111 963 1107 1266 1416 1800 2043
Size: 98 13 852 144 159 150 384 243
Cuts at: 2043 2522 3054 3208 3339
Size: 479 532 154 131
Fragments arranged by size:
852 532 479 384 243 159 154 150
144 131 98 13
NaeI GCC'GGC
Cuts at: 0 2821 3339
Size: 2821 518
NarI GG'CG_CC
Cuts at: 0 2285 3339
Size: 2285 1054
NdeI CA'TA_TG
Cuts at: 0 186 281 3339
Size: 186 95 3058
Fragments arranged by size:
3058 186 95
NgoMIV G'CCGG_C
Cuts at: 0 2819 3339
Size: 2819 520
NspI r_CATG'y
Cuts at: 0 397 1290 3339
Size: 397 893 2049
Fragments arranged by size:
2049 893 397
PasI CC'CwG_GG
Cuts at: 0 1089 1824 2742 3339
Size: 1089 735 918 597
Fragments arranged by size:
1089 918 735 597
PflMI CCAn_nnn'nTGG
Cuts at: 0 98 199 3339
Size: 98 101 3140
Fragments arranged by size:
3140 101 98
PfoI T'CCnGG_A
Cuts at: 0 1454 3339
Size: 1454 1885
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 541 550 573 3339
Size: 518 23 9 23 2766
Fragments arranged by size:
2766 518 23 23 9
PpuMI rG'GwC_Cy
Cuts at: 0 1137 2797 3339
Size: 1137 1660 542
Fragments arranged by size:
1660 1137 542
PspXI vC'TCGA_Gb
Cuts at: 0 251 3339
Size: 251 3088
PstI C_TGCA'G
Cuts at: 0 557 1145 1433 1556 2987 3330 3339
Size: 557 588 288 123 1431 343 9
Fragments arranged by size:
1431 588 557 343 288 123 9
PvuII CAG'CTG
Cuts at: 0 98 963 1107 1266 1416 1800 2043 2522
Size: 98 865 144 159 150 384 243 479
Cuts at: 2522 3054 3208 3339
Size: 532 154 131
Fragments arranged by size:
865 532 479 384 243 159 154 150
144 131 98
SacI G_AGCT'C
Cuts at: 0 844 2387 3044 3339
Size: 844 1543 657 295
Fragments arranged by size:
1543 844 657 295
SacII CC_GC'GG
Cuts at: 0 112 3339
Size: 112 3227
SalI G'TCGA_C
Cuts at: 0 2328 3339
Size: 2328 1011
SbfI CC_TGCA'GG
Cuts at: 0 1145 1556 2987 3339
Size: 1145 411 1431 352
Fragments arranged by size:
1431 1145 411 352
SexAI A'CCwGG_T
Cuts at: 0 296 341 3339
Size: 296 45 2998
Fragments arranged by size:
2998 296 45
SfcI C'TryA_G
Cuts at: 0 553 1141 1429 1552 2983 3326 3339
Size: 553 588 288 123 1431 343 13
Fragments arranged by size:
1431 588 553 343 288 123 13
SfoI GGC'GCC
Cuts at: 0 2286 3339
Size: 2286 1053
SmaI CCC'GGG
Cuts at: 0 508 1153 3339
Size: 508 645 2186
Fragments arranged by size:
2186 645 508
SmlI C'TyrA_G
Cuts at: 0 251 651 1278 2229 2386 3043 3339
Size: 251 400 627 951 157 657 296
Fragments arranged by size:
951 657 627 400 296 251 157
SphI G_CATG'C
Cuts at: 0 397 1290 3339
Size: 397 893 2049
Fragments arranged by size:
2049 893 397
SspI AAT'ATT
Cuts at: 0 2030 3339
Size: 2030 1309
StuI AGG'CCT
Cuts at: 0 985 3339
Size: 985 2354
StyI C'CwwG_G
Cuts at: 0 1691 1740 3212 3339
Size: 1691 49 1472 127
Fragments arranged by size:
1691 1472 127 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1174 1912 1960 2913 3173 3339
Size: 1174 738 48 953 260 166
Fragments arranged by size:
1174 953 738 260 166 48
TatI w'GTAC_w
Cuts at: 0 255 3339
Size: 255 3084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2823 2855 3339
Size: 2823 32 484
Fragments arranged by size:
2823 484 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1242 3339
Size: 1242 2097
XhoI C'TCGA_G
Cuts at: 0 251 3339
Size: 251 3088
XmaI C'CCGG_G
Cuts at: 0 506 1151 3339
Size: 506 645 2188
Fragments arranged by size:
2188 645 506
ZraI GAC'GTC
Cuts at: 0 3336 3339
Size: 3336 3
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PasI PflMI PfoI
PpiI PpuMI PspXI PstI PvuII SacI SacII SalI
SbfI SexAI SfcI SfoI SmaI SmlI SphI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AclI AfeI AgeI AloI ApaI AscI AsiSI AvrII
BaeI BcgI BclI BfrBI BlpI BmtI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BstEII BstZ17I BtgZI DraI
DraIII EcoRV FseI FspI FspAI HpaI MluI NcoI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspOMI PsrI PvuI RsrII SanDI SapI
ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI
Tth111I XbaI XmnI