Restriction map for
pFN21AE0660
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII ItaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI StyI SwaI TseI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AcsI 264 AAATTT [GA]^AATT[CT]
AluI 1155 AGCT AG^CT
ApoI 264 AAATTT [GA]^AATT[CT]
AspHI 564 GAGCAC G[AT]GC[AT]^C
AvaII 511 GGTCC G^G[AT]CC
BamHI 63 GGATCC G^GATCC
BglII 733 AGATCT A^GATCT
BsaJI 175 CCTGGG C^C[ACGT][ACGT]GG
BseRI 46 GAGGAG GAGGAG(10/8)
BsiHKAI 564 GAGCAC G[AT]GC[AT]^C
BsmI 310 GAATGC GAATGC(1/-1)
BsrDI 424 CATTGC GCAATG(2/0)
BstXI 794 CCACTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 716 CTCTTC CTCTTC(1/4)
EcoT22I 674 ATGCAT ATGCA^T
HincII 466 GTTGAC GT[CT]^[GA]AC
HindII 466 GTTGAC GT[CT]^[GA]AC
HphI 458 TCACC GGTGA(8/7)
Ksp632I 716 CTCTTC CTCTTC(1/4)
MseI 221 TTAA T^TAA
NdeI 336 CATATG CA^TATG
NsiI 674 ATGCAT ATGCA^T
NspI 676 GCATGT [GA]CATG^[CT]
SfaNI 96 GATGC GCATC(5/9)
SpeI 490 ACTAGT A^CTAGT
SspI 956 AATATT AAT^ATT
Tru9I 221 TTAA T^TAA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 64 GATCC GGATC(4/5)
63 GGATC
BbsI 142 GAAGAC GAAGAC(2/6)
331 GTCTTC
BmyI 564 GAGCAC G[AGT]GC[ACT]^C
1049 GTGCCC
BpuAI 142 GAAGAC GAAGAC(2/6)
331 GTCTTC
BsiYI 350 CCCTTCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
502 CCTTATAAGGG
BslI 350 CCCTTCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
502 CCTTATAAGGG
BsmAI 207 GAGAC GTCTC(1/5)
369 GTCTC
Bsp1286I 564 GAGCAC G[AGT]GC[ACT]^C
1049 GTGCCC
BstBI 1055 TTCGAA TT^CGAA
1139 TTCGAA
BstF5I 363 CATCC GGATG(2/0)
1012 GGATG
BstYI 63 GGATCC [GA]^GATC[CT]
733 AGATCT
Cfr13I 499 GGGCC G^G[ACGT]CC
511 GGTCC
DpnII 64 GATC ^GATC
734 GATC
FokI 363 CATCC GGATG(9/13)
1012 GGATG
HaeIII 33 GGCC GG^CC
500 GGCC
MaeIII 481 GTAAC ^GT[ACGT]AC
965 GTAAC
MboI 64 GATC ^GATC
734 GATC
MflI 63 GGATCC [GA]^GATC[CT]
733 AGATCT
NdeII 64 GATC ^GATC
734 GATC
NspV 1055 TTCGAA TT^CGAA
1139 TTCGAA
Sau3AI 64 GATC ^GATC
734 GATC
Sau96I 499 GGGCC G^G[ACGT]CC
511 GGTCC
SfcI 84 CTACAG C^T[GA][CT]AG
125 CTGTAG
SfuI 1055 TTCGAA TT^CGAA
1139 TTCGAA
TaqI 1056 TCGA T^CGA
1140 TCGA
TthHB8I 1056 TCGA T^CGA
1140 TCGA
XhoII 63 GGATCC [GA]^GATC[CT]
733 AGATCT
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
BfaI BpmI BsrI BssKI BstNI
DdeI EcoRII HinfI MaeI MboII
MnlI MslI MvaI NlaIII NlaIV
PleI ScrFI TfiI Tsp509I TspRI