Restriction map for
pFN21AE1007
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HapII HincII HindII HindIII
HpaI HpaII KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MroI MseI MslI MspI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 367 GACGTC GACGT^C
AccI 205 GTAGAC GT^[AC][GT]AC
AccII 202 CGCG CG^CG
AhdI 553 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 358 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 553 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 505 CCCGAG C^[CT]CG[GA]G
AviII 539 TGCGCA TGC^GCA
BalI 53 TGGCCA TGG^CCA
BanI 511 GGCGCC G^G[CT][GA]CC
BbeI 511 GGCGCC GGCGC^C
BglI 55 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 492 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 492 GCTCAGC GC^T[ACGT]AGC
BsaI 321 GGTCTC GGTCTC(1/5)
BsmAI 322 GTCTC GTCTC(1/5)
BsmFI 198 GTCCC GGGAC(10/14)
BsoBI 505 CCCGAG C^[CT]CG[GA]G
BstAPI 348 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 202 CGCG CG^CG
CelII 492 GCTCAGC GC^T[ACGT]AGC
DpnII 456 GATC ^GATC
Eam1105I 553 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 200 CCCGC CCCGC(4/6)
FspI 539 TGCGCA TGC^GCA
HaeII 511 GGCGCC [GA]GCGC^[CT]
KasI 511 GGCGCC G^GCGCC
MaeII 368 ACGT A^CGT
MboI 456 GATC ^GATC
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
MvnI 202 CGCG CG^CG
NarI 511 GGCGCC GG^CGCC
NcoI 305 CCATGG C^CATGG
NdeII 456 GATC ^GATC
NlaIV 511 GGCGCC GG[ACGT]^[ACGT]CC
Sau3AI 456 GATC ^GATC
SfoI 511 GGCGCC GGC^GCC
SmlI 579 CTTGAG C^T[CT][GA]AG
TaqI 344 TCGA T^CGA
TthHB8I 344 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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BanII 399 GAGCTC G[GA]GC[CT]^C
531 GAGCTC
BpmI 43 CTGGAG CTGGAG(16/14)
475 CTGGAG
BseRI 324 CTCCTC GAGGAG(10/8)
463 GAGGAG
BsgI 226 GTGCAG GTGCAG(16/14)
355 GTGCAG
BsrI 182 ACTGG ACTGG(1/-1)
18 CCAGT
BstEII 252 GGTGACC G^GT[ACGT]ACC
273 GGTGACC
BstF5I 156 CATCC GGATG(2/0)
191 GGATG
BstPI 252 GGTGACC G^GT[ACGT]ACC
273 GGTGACC
BstXI 257 CCAAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
278 CCAAAGCCCTGG
CfoI 512 GCGC GCG^C
540 GCGC
DraII 104 AGGCCCT [GA]G^G[ACGT]CC[CT]
293 AGGCCCT
DsaI 305 CCATGG C^C[GA][CT]GG
521 CCGTGG
EcoNI 285 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
312 CCTGCAGAAGG
EcoO109I 104 AGGCCCT [GA]G^G[ACGT]CC[CT]
293 AGGCCCT
EcoO65I 252 GGTGACC G^GT[ACGT]ACC
273 GGTGACC
FokI 156 CATCC GGATG(9/13)
191 GGATG
HgaI 208 GACGC GACGC(5/10)
196 GCGTC
HhaI 512 GCGC GCG^C
540 GCGC
HinP1I 512 GCGC G^CGC
540 GCGC
HinfI 73 GACTC G^A[ACGT]TC
391 GACTC
MboII 99 GAAGA GAAGA(8/7)
165 GAAGA
PleI 73 GACTC GAGTC(4/5)
391 GACTC
PstI 112 CTGCAG CTGCA^G
313 CTGCAG
SacI 399 GAGCTC GAGCT^C
531 GAGCTC
SfcI 112 CTGCAG C^T[GA][CT]AG
313 CTGCAG
TspRI 212 CAGTG CA[GC]TG[ACGT][ACGT]^
329 CAGTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcyI AluI AspHI AvaII
BbvI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BspMI BssKI
BstNI Cac8I Cfr13I DdeI EaeI
EcoRII EcoT14I Fnu4HI HaeIII Hin1I
HphI ItaI MaeIII MnlI MspA1I
MvaI MwoI NlaIII PvuII Sau96I
ScrFI StyI TseI Tsp45I