################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AlwI AlwNI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaII AviII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AhdI 178 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 628 GTGCAC G^TGCAC Aor51HI 38 AGCGCT AGC^GCT ApaLI 628 GTGCAC G^TGCAC AspEI 178 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 6 CCCGGG C^[CT]CG[GA]G AvrII 102 CCTAGG C^CTAGG BanI 202 GGCGCC G^G[CT][GA]CC BbeI 202 GGCGCC GGCGC^C BglI 158 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 102 CCTAGG C^CTAGG BpmI 118 CTGGAG CTGGAG(16/14) BsgI 91 CTGCAC GTGCAG(16/14) BsiEI 161 CGGCCG CG[GA][CT]^CG BsmAI 421 GAGAC GTCTC(1/5) BsoBI 6 CCCGGG C^[CT]CG[GA]G BspHI 639 TCATGA T^CATGA BssHII 289 GCGCGC G^CGCGC BssSI 631 CACGAG CACGAG(-5/-1) DpnII 515 GATC ^GATC DraIII 715 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 769 CCATGG C^C[GA][CT]GG EaeI 161 CGGCCG [CT]^GGCC[GA] EagI 161 CGGCCG C^GGCCG Eam1105I 178 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 21 GAAGAG CTCTTC(1/4) EclXI 161 CGGCCG C^GGCCG Eco47III 38 AGCGCT AGC^GCT Eco52I 161 CGGCCG C^GGCCG KasI 202 GGCGCC G^GCGCC Ksp632I 21 GAAGAG CTCTTC(1/4) MaeII 608 ACGT A^CGT MboI 515 GATC ^GATC MslI 218 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 202 GGCGCC GG^CGCC NcoI 769 CCATGG C^CATGG NdeII 515 GATC ^GATC RcaI 639 TCATGA T^CATGA SacI 710 GAGCTC GAGCT^C SapI 21 GAAGAGC GCTCTTC(1/4) Sau3AI 515 GATC ^GATC ScaI 442 AGTACT AGT^ACT SfaNI 543 GCATC GCATC(5/9) SfoI 202 GGCGCC GGC^GCC SmaI 6 CCCGGG CCC^GGG XmaCI 6 CCCGGG C^CCGGG XmaI 6 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 183 GGCGTC G[GA]^CG[CT]C 202 GGCGCC AspHI 628 GTGCAC G[AT]GC[AT]^C 710 GAGCTC BbsI 597 GAAGAC GAAGAC(2/6) 668 GAAGAC BfaI 103 CTAG C^TAG 479 CTAG BpuAI 597 GAAGAC GAAGAC(2/6) 668 GAAGAC BsaHI 183 GGCGTC G[GA]^CG[CT]C 202 GGCGCC BsiHKAI 628 GTGCAC G[AT]GC[AT]^C 710 GAGCTC BsrBI 620 CCGCTC CCGCTC(-3/-3) 12 GAGCGG DdeI 446 CTGAG C^T[ACGT]AG 759 CTCAG EcoT14I 102 CCTAGG C^C[AT][AT]GG 769 CCATGG HaeII 38 AGCGCT [GA]GCGC^[CT] 202 GGCGCC Hin1I 183 GGCGTC G[GA]^CG[CT]C 202 GGCGCC HphI 18 GGTGA GGTGA(8/7) 433 TCACC MaeI 103 CTAG C^TAG 479 CTAG StyI 102 CCTAGG C^C[AT][AT]GG 769 CCATGG TaqI 453 TCGA T^CGA 520 TCGA TfiI 636 GATTC G^A[AT]TC 643 GAATC TspRI 368 CACTG CA[GC]TG[ACGT][ACGT]^ 374 CAGTG TthHB8I 453 TCGA T^CGA 520 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI ApaI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PleI RsaI Sau96I ScrFI TseI