(Circular) (Six-base) MAPSORT of: pFN21AB9292.seq Check: 209 from: 1 to: 5515 pFN21AB9292 5515 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AfeI AGC'GCT Cuts at: 2045 2045 Size: 5515 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 2188 4244 2188 Size: 2056 3459 AleI CACnn'nnGTG Cuts at: 1166 2227 1166 Size: 1061 4454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2245 2487 2571 1184 Size: 1061 242 84 4128 Fragments arranged by size: 4128 1061 242 84 AlwNI CAG_nnn'CTG Cuts at: 3258 4830 3258 Size: 1572 3943 ApaI G_GGCC'C Cuts at: 2165 2219 2305 2165 Size: 54 86 5375 Fragments arranged by size: 5375 86 54 ApaLI G'TGCA_C Cuts at: 2633 3572 4728 2633 Size: 939 1156 3420 Fragments arranged by size: 3420 1156 939 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2840 1870 Size: 73 68 829 4545 Fragments arranged by size: 4545 829 73 68 AvrII C'CTAG_G Cuts at: 2107 2107 Size: 5515 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2836 4291 618 Size: 325 217 627 420 629 1455 1842 Fragments arranged by size: 1842 1455 629 627 420 325 217 BanII G_rGCy'C Cuts at: 1391 2165 2219 2305 2719 2834 1391 Size: 774 54 86 414 115 4072 Fragments arranged by size: 4072 774 414 115 86 54 BbeI G_GCGC'C Cuts at: 2211 2211 Size: 5515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2609 2680 928 Size: 971 710 71 3763 Fragments arranged by size: 3763 971 710 71 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2169 4126 136 Size: 107 122 71 631 605 497 1957 1525 Fragments arranged by size: 1957 1525 631 605 497 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2165 2219 2305 2637 3576 4732 1790 Size: 375 54 86 332 939 1156 2573 Fragments arranged by size: 2573 1156 939 375 332 86 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2167 3246 4204 Size: 416 53 83 399 24 750 1079 958 Cuts at: 4204 442 Size: 1753 Fragments arranged by size: 1753 1079 958 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 4175 1239 Size: 905 2031 2579 Fragments arranged by size: 2579 2031 905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3640 4505 4803 3640 Size: 865 298 4352 Fragments arranged by size: 4352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2189 2208 3322 3704 Size: 53 83 186 936 656 19 1114 382 Cuts at: 3704 4339 275 Size: 635 1451 Fragments arranged by size: 1451 1114 936 656 635 382 186 83 53 19 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5290 Fragments arranged by size: 5290 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2164 2291 2324 2752 1186 Size: 895 83 127 33 428 3949 Fragments arranged by size: 3949 895 428 127 83 33 BseYI C'CCAG_C Cuts at: 2061 2568 4718 5214 2061 Size: 507 2150 496 2362 Fragments arranged by size: 2362 2150 507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 1163 Size: 573 345 4597 Fragments arranged by size: 4597 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2169 2965 3726 4754 1540 Size: 275 186 168 796 761 1028 2301 Fragments arranged by size: 2301 1028 796 761 275 186 168 BsiHKAI G_wGCw'C Cuts at: 2637 2719 3576 3661 4732 2637 Size: 82 857 85 1071 3420 Fragments arranged by size: 3420 1071 857 85 82 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2219 2305 2637 2719 2834 3576 Size: 399 375 54 86 332 82 115 742 Cuts at: 3576 3661 4732 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 332 115 86 85 82 54 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 2644 3404 5134 2644 Size: 760 1730 3025 Fragments arranged by size: 3025 1730 760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2019 2627 3402 1394 Size: 429 196 608 775 3507 Fragments arranged by size: 3507 775 608 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2115 2168 2263 4159 4346 1700 Size: 110 305 53 95 1896 187 2869 Fragments arranged by size: 2869 1896 305 187 110 95 53 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 2294 1925 Size: 369 5146 BssSI C'ACGA_G Cuts at: 2636 3575 4587 2636 Size: 939 1012 3564 Fragments arranged by size: 3564 1012 939 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510 Size: 873 367 385 17 1452 86 12 346 Cuts at: 5510 1972 Size: 1977 Fragments arranged by size: 1977 1452 873 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2774 513 Size: 552 1709 3254 Fragments arranged by size: 3254 1709 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 2372 3031 3825 3845 2372 Size: 659 794 20 4042 Fragments arranged by size: 4042 794 659 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DraIII CAC_nnn'GTG Cuts at: 2725 2725 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2962 3851 Size: 54 1425 50 275 18 336 796 889 Cuts at: 3851 5480 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 796 336 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2166 2962 1537 Size: 275 354 796 4090 Fragments arranged by size: 4090 796 354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 3445 1353 Size: 668 1424 3423 Fragments arranged by size: 3423 1424 668 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2297 4102 4476 4622 387 Size: 1064 138 708 1805 374 146 1280 Fragments arranged by size: 1805 1280 1064 708 374 146 138 EcoICRI GAG'CTC Cuts at: 2717 2717 Size: 5515 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 3578 4175 4962 5230 1239 Size: 393 512 1434 597 787 268 1524 Fragments arranged by size: 1524 1434 787 597 512 393 268 EcoO109I rG'GnC_Cy Cuts at: 2161 2215 2216 2301 2302 2161 Size: 54 1 85 1 5374 Fragments arranged by size: 5374 85 54 1 1 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2047 2211 4662 1678 Size: 369 164 2451 2531 Fragments arranged by size: 2531 2451 369 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 4156 4188 4230 Size: 32 135 32 602 32 1950 32 42 Cuts at: 4230 4262 1373 Size: 32 2626 Fragments arranged by size: 2626 1950 602 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KasI G'GCGC_C Cuts at: 2207 2207 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3693 Fragments arranged by size: 3693 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2227 3473 3832 3991 518 Size: 648 1061 1246 359 159 2042 Fragments arranged by size: 2042 1246 1061 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2931 3608 4756 5001 1606 Size: 1325 677 1148 245 2120 Fragments arranged by size: 2120 1325 1148 677 245 NaeI GCC'GGC Cuts at: 2117 2170 2265 2117 Size: 53 95 5367 Fragments arranged by size: 5367 95 53 NarI GG'CG_CC Cuts at: 2208 2208 Size: 5515 NcoI C'CATG_G Cuts at: 513 1065 2774 513 Size: 552 1709 3254 Fragments arranged by size: 3254 1709 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NgoMIV G'CCGG_C Cuts at: 2115 2168 2263 2115 Size: 53 95 5367 Fragments arranged by size: 5367 95 53 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2873 4418 1132 Size: 97 1644 1545 2229 Fragments arranged by size: 2229 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2161 2215 2301 2161 Size: 54 86 5375 Fragments arranged by size: 5375 86 54 PstI C_TGCA'G Cuts at: 830 2867 830 Size: 2037 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 SacI G_AGCT'C Cuts at: 2719 2719 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SapI GCTCTTCn'nnn_ Cuts at: 2021 2021 Size: 5515 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 2449 3763 5475 2449 Size: 1314 1712 2489 Fragments arranged by size: 2489 1712 1314 SfcI C'TryA_G Cuts at: 826 1045 2863 3998 4679 4870 826 Size: 219 1818 1135 681 191 1471 Fragments arranged by size: 1818 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2209 2209 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2013 2842 1872 Size: 141 829 4545 Fragments arranged by size: 4545 829 141 SmlI C'TyrA_G Cuts at: 820 1017 1943 3619 4520 4782 820 Size: 197 926 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5515 StyI C'CwwG_G Cuts at: 513 1065 2107 2774 2954 513 Size: 552 1042 667 180 3074 Fragments arranged by size: 3074 1042 667 552 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2455 2600 3542 3559 3712 3897 4342 Size: 1255 875 145 942 17 153 185 445 Cuts at: 4342 325 Size: 1498 Fragments arranged by size: 1498 1255 942 875 445 185 153 145 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 2447 3761 Size: 274 80 33 51 1443 57 413 1314 Cuts at: 3761 5473 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1314 413 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2011 2840 1870 Size: 141 829 4545 Fragments arranged by size: 4545 829 141 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI