Restriction map for
pFN21AE0636
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NlaIV NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 491 GCGG CCGC(-3/-1)
AfaI 1114 GTAC GT^AC
AflIII 780 ACACGT A^C[GA][CT]GT
AlwI 168 GATCC GGATC(4/5)
AlwNI 236 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 693 GGTCC G^G[AT]CC
BanII 228 GAGCTC G[GA]GC[CT]^C
BbrPI 781 CACGTG CAC^GTG
BclI 2 TGATCA T^GATCA
BsaAI 781 CACGTG [CT]AC^GT[GA]
BsaI 636 GAGACC GGTCTC(1/5)
BsgI 732 GTGCAG GTGCAG(16/14)
BsmFI 1017 GTCCC GGGAC(10/14)
Bsp1407I 1113 TGTACA T^GTACA
BsrGI 1113 TGTACA T^GTACA
Cfr13I 693 GGTCC G^G[ACGT]CC
DsaI 196 CCATGG C^C[GA][CT]GG
EcoNI 584 CCTATAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 956 GAATTC G^AATTC
EcoT14I 196 CCATGG C^C[AT][AT]GG
EcoT22I 673 ATGCAT ATGCA^T
FauI 491 GCGGG CCCGC(4/6)
FbaI 2 TGATCA T^GATCA
HindIII 917 AAGCTT A^AGCTT
MaeII 782 ACGT A^CGT
MspA1I 67 CAGCTG C[AC]G^C[GT]G
NcoI 196 CCATGG C^CATGG
NdeI 906 CATATG CA^TATG
NsiI 673 ATGCAT ATGCA^T
PmaCI 781 CACGTG CAC^GTG
PmlI 781 CACGTG CAC^GTG
PstI 53 CTGCAG CTGCA^G
PvuII 67 CAGCTG CAG^CTG
RsaI 1114 GTAC GT^AC
SacI 228 GAGCTC GAGCT^C
Sau96I 693 GGTCC G^G[ACGT]CC
SfaNI 273 GCATC GCATC(5/9)
SspBI 1113 TGTACA T^GTACA
StyI 196 CCATGG C^C[AT][AT]GG
Tsp45I 22 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 228 GAGCTC G[AT]GC[AT]^C
363 GAGCAC
BbvI 66 GCAGC GCAGC(8/12)
69 GCTGC
BmyI 228 GAGCTC G[AGT]GC[ACT]^C
363 GAGCAC
BpmI 233 CTCCAG CTGGAG(16/14)
713 CTGGAG
BsaJI 196 CCATGG C^C[ACGT][ACGT]GG
265 CCAGGG
BsiHKAI 228 GAGCTC G[AT]GC[AT]^C
363 GAGCAC
BsmAI 636 GAGAC GTCTC(1/5)
702 GAGAC
Bsp1286I 228 GAGCTC G[AGT]GC[ACT]^C
363 GAGCAC
BsrDI 566 GCAATG GCAATG(2/0)
650 GCAATG
BsrI 712 ACTGG ACTGG(1/-1)
897 CCAGT
BssSI 223 CACGAG CACGAG(-5/-1)
688 CTCGTG
BstAPI 482 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
598 GCACACCTTGC
DpnII 3 GATC ^GATC
168 GATC
Fnu4HI 66 GCAGC GC^[ACGT]GC
69 GCTGC
ItaI 66 GCAGC GC^[ACGT]GC
69 GCTGC
MboI 3 GATC ^GATC
168 GATC
MwoI 482 GCAGTGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
598 GCACACCTTGC
NdeII 3 GATC ^GATC
168 GATC
NlaIII 197 CATG CATG^
529 CATG
PflMI 30 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
684 CCAACTCGTGG
Sau3AI 3 GATC ^GATC
168 GATC
SmlI 172 CTCAAG C^T[CT][GA]AG
799 CTTGAG
TseI 66 GCAGC G^C[AT]GC
69 GCTGC
Van91I 30 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
684 CCAACTCGTGG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BseRI
BsiYI BslI BsmI BssKI BstF5I
BstNI DdeI EcoRII FokI HinfI
MaeIII MnlI MseI MslI MvaI
ScrFI SfcI TfiI Tru9I Tsp509I
TspRI XmnI