(Circular) (Six-base) MAPSORT of: pFN21AE0636.seq Check: 2758 from: 1 to: 5839 pFN21AE0636 5839 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2827 3902 5286 5554 1632 Size: 1195 1075 1384 268 1917 Fragments arranged by size: 1917 1384 1195 1075 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 2785 4738 5715 1203 Size: 1582 1953 977 1327 Fragments arranged by size: 1953 1582 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 2939 1166 Size: 1773 4066 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 2246 3582 5154 2246 Size: 1336 1572 2931 Fragments arranged by size: 2931 1572 1336 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 2331 2625 2961 3129 3148 3374 3526 5635 Size: 838 294 336 168 19 226 152 2109 Cuts at: 5635 1493 Size: 1697 Fragments arranged by size: 2109 1697 838 336 294 226 168 152 19 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 3164 1870 Size: 73 1221 4545 Fragments arranged by size: 4545 1221 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2890 2923 2890 Size: 33 5806 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 2237 3158 1391 Size: 846 921 4072 Fragments arranged by size: 4072 921 846 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 5380 BfrBI ATG'CAT Cuts at: 2680 2680 Size: 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4450 136 Size: 107 122 71 631 605 2778 1525 Fragments arranged by size: 2778 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 3900 5056 1790 Size: 2110 1156 2573 Fragments arranged by size: 2573 2110 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2223 2739 4499 1239 Size: 984 516 1760 2579 Fragments arranged by size: 2579 1760 984 516 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2162 2825 3964 4829 5127 2162 Size: 663 1139 865 298 2874 Fragments arranged by size: 2874 1139 865 663 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2635 4502 882 Size: 617 1136 1867 2219 Fragments arranged by size: 2219 1867 1136 617 BsaAI yAC'GTr Cuts at: 493 1204 2788 3630 493 Size: 711 1584 842 2702 Fragments arranged by size: 2702 1584 842 711 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3646 4028 4663 275 Size: 53 83 186 936 2113 382 635 1451 Fragments arranged by size: 2113 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5614 Fragments arranged by size: 5614 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2761 3020 1186 Size: 892 683 259 4005 Fragments arranged by size: 4005 892 683 259 BseYI C'CCAG_C Cuts at: 5042 5538 5042 Size: 496 5343 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2758 1163 Size: 573 1022 4244 Fragments arranged by size: 4244 1022 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2372 3900 3985 5056 2237 Size: 135 1528 85 1071 3020 Fragments arranged by size: 3020 1528 1071 135 85 BsmI GAATG_Cn' Cuts at: 2489 2498 2573 3350 3443 2489 Size: 9 75 777 93 4885 Fragments arranged by size: 4885 777 93 75 9 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2372 3158 3900 3985 5056 1391 Size: 399 447 135 786 742 85 1071 2174 Fragments arranged by size: 2174 1071 786 742 447 399 135 85 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3194 844 Size: 2350 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3726 1394 Size: 429 1903 3507 Fragments arranged by size: 3507 1903 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2578 2662 4334 4508 65 Size: 1183 1330 84 1672 174 1396 Fragments arranged by size: 1672 1396 1330 1183 174 84 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 3118 96 Size: 3022 2817 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 2228 2693 3899 4911 2228 Size: 465 1206 1012 3156 Fragments arranged by size: 3156 1206 1012 465 BstAPI GCAn_nnn'nTGC Cuts at: 794 2493 2609 794 Size: 1699 116 4024 Fragments arranged by size: 4024 1699 116 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstYI r'GATC_y Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834 Size: 1197 367 385 17 1452 86 12 346 Cuts at: 5834 1972 Size: 1977 Fragments arranged by size: 1977 1452 1197 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2201 513 Size: 552 1136 4151 Fragments arranged by size: 4151 1136 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5100 Fragments arranged by size: 5100 683 56 BtsI GCAGTG_nn' Cuts at: 2055 2494 3355 4149 4169 2055 Size: 439 861 794 20 3725 Fragments arranged by size: 3725 861 794 439 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2560 2595 2644 2679 529 Size: 35 1996 35 49 35 3689 Fragments arranged by size: 3689 1996 49 35 35 35 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804 Size: 54 1425 50 275 18 1456 889 1629 Cuts at: 5804 8 Size: 43 Fragments arranged by size: 1629 1456 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3769 1353 Size: 2416 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2235 2235 Size: 5839 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2739 2827 3902 4499 5286 5554 Size: 393 591 516 88 1075 597 787 268 Cuts at: 5554 1239 Size: 1524 Fragments arranged by size: 1524 1075 787 597 591 516 393 268 88 EcoNI CCTnn'n_nnAGG Cuts at: 2593 2593 Size: 5839 EcoRI G'AATT_C Cuts at: 2961 3148 3526 2961 Size: 187 378 5274 Fragments arranged by size: 5274 378 187 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 4986 1678 Size: 3308 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2359 2391 4480 4512 4554 Size: 32 135 32 787 32 2089 32 42 Cuts at: 4554 4586 1373 Size: 32 2626 Fragments arranged by size: 2626 2089 787 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3429 669 Size: 988 281 1245 246 3079 Fragments arranged by size: 3079 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 2922 748 Size: 2174 3665 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 4953 5137 1305 Size: 792 2856 184 2007 Fragments arranged by size: 2856 2007 792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4017 Fragments arranged by size: 4017 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2818 2939 3797 4156 4315 518 Size: 648 975 677 121 858 359 159 2042 Fragments arranged by size: 2042 975 858 677 648 359 159 121 MspA1I CmG'CkG Cuts at: 1606 2074 3255 3932 5080 5325 1606 Size: 468 1181 677 1148 245 2120 Fragments arranged by size: 2120 1181 1148 677 468 245 NcoI C'CATG_G Cuts at: 513 1065 2201 513 Size: 552 1136 4151 Fragments arranged by size: 4151 1136 552 NdeI CA'TA_TG Cuts at: 387 2912 387 Size: 2525 3314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NsiI A_TGCA'T Cuts at: 2682 2682 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 3197 4742 1132 Size: 97 1968 1545 2229 Fragments arranged by size: 2229 1968 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 2695 1108 Size: 933 654 4252 Fragments arranged by size: 4252 933 654 PfoI T'CCnGG_A Cuts at: 2016 2034 2016 Size: 18 5821 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PmlI CAC'GTG Cuts at: 2788 2788 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 2361 2865 3409 2361 Size: 504 544 4791 Fragments arranged by size: 4791 544 504 PstI C_TGCA'G Cuts at: 830 2062 3191 830 Size: 1232 1129 3478 Fragments arranged by size: 3478 1232 1129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5839 SacI G_AGCT'C Cuts at: 2237 2237 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2058 2125 2466 2590 3187 4322 5003 Size: 219 1013 67 341 124 597 1135 681 Cuts at: 5003 5194 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1013 681 597 341 219 191 124 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 2804 3943 4844 5106 820 Size: 197 926 234 627 1139 901 262 1553 Fragments arranged by size: 1553 1139 926 901 627 262 234 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 2201 3278 513 Size: 552 1136 1077 3074 Fragments arranged by size: 3074 1136 1077 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3866 3883 4036 4221 4666 325 Size: 1255 2286 17 153 185 445 1498 Fragments arranged by size: 2286 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3118 4085 5797 Size: 274 80 33 51 1443 1141 967 1712 Cuts at: 5797 96 Size: 138 Fragments arranged by size: 1712 1443 1141 967 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5799 5831 5799 Size: 32 5807 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 2504 2756 2840 3968 2504 Size: 252 84 1128 4375 Fragments arranged by size: 4375 1128 252 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI