Restriction map for
pFN21AE0636
(Linear) (Six-base) MAPSORT of: KIEE0636.seq Check: 6122 from: 1 to: 1134
KIEE0636.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 1134
Size: 822 312
AflIII A'CryG_T
Cuts at: 0 780 1134
Size: 780 354
AleI CACnn'nnGTG
Cuts at: 0 934 1134
Size: 934 200
AlwNI CAG_nnn'CTG
Cuts at: 0 241 1134
Size: 241 893
ApoI r'AATT_y
Cuts at: 0 326 620 956 1124 1134
Size: 326 294 336 168 10
Fragments arranged by size:
336 326 294 168 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 885 918 1134
Size: 885 33 216
Fragments arranged by size:
885 216 33
BanII G_rGCy'C
Cuts at: 0 232 1134
Size: 232 902
BclI T'GATC_A
Cuts at: 0 2 1134
Size: 2 1132
BfrBI ATG'CAT
Cuts at: 0 675 1134
Size: 675 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 734 1134
Size: 218 516 400
Fragments arranged by size:
516 400 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 820 1134
Size: 157 663 314
Fragments arranged by size:
663 314 157
BsaI GGTCTCn'nnnn_
Cuts at: 0 630 1134
Size: 630 504
BsaAI yAC'GTr
Cuts at: 0 783 1134
Size: 783 351
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 756 1015 1134
Size: 73 683 259 119
Fragments arranged by size:
683 259 119 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 753 1134
Size: 753 381
BsiHKAI G_wGCw'C
Cuts at: 0 232 367 1134
Size: 232 135 767
Fragments arranged by size:
767 232 135
BsmI GAATG_Cn'
Cuts at: 0 484 493 568 1134
Size: 484 9 75 566
Fragments arranged by size:
566 484 75 9
Bsp1286I G_dGCh'C
Cuts at: 0 232 367 1134
Size: 232 135 767
Fragments arranged by size:
767 232 135
BsrDI GCAATG_nn'
Cuts at: 0 573 657 1134
Size: 573 84 477
Fragments arranged by size:
573 477 84
BsrGI T'GTAC_A
Cuts at: 0 1113 1134
Size: 1113 21
BssSI C'ACGA_G
Cuts at: 0 223 688 1134
Size: 223 465 446
Fragments arranged by size:
465 446 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 604 1134
Size: 488 116 530
Fragments arranged by size:
530 488 116
BtgI C'CryG_G
Cuts at: 0 196 1134
Size: 196 938
BtsI GCAGTG_nn'
Cuts at: 0 50 489 1134
Size: 50 439 645
Fragments arranged by size:
645 439 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 555 590 639 674 1134
Size: 555 35 49 35 460
Fragments arranged by size:
555 460 49 35 35
EcoICRI GAG'CTC
Cuts at: 0 230 1134
Size: 230 904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 734 822 1134
Size: 218 516 88 312
Fragments arranged by size:
516 312 218 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 588 1134
Size: 588 546
EcoRI G'AATT_C
Cuts at: 0 956 1134
Size: 956 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 1134
Size: 354 32 748
Fragments arranged by size:
748 354 32
HindIII A'AGCT_T
Cuts at: 0 917 1134
Size: 917 217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1134
Size: 92 1042
MslI CAynn'nnrTG
Cuts at: 0 136 813 934 1134
Size: 136 677 121 200
Fragments arranged by size:
677 200 136 121
MspA1I CmG'CkG
Cuts at: 0 69 1134
Size: 69 1065
NcoI C'CATG_G
Cuts at: 0 196 1134
Size: 196 938
NdeI CA'TA_TG
Cuts at: 0 907 1134
Size: 907 227
NsiI A_TGCA'T
Cuts at: 0 677 1134
Size: 677 457
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 690 1134
Size: 36 654 444
Fragments arranged by size:
654 444 36
PfoI T'CCnGG_A
Cuts at: 0 11 29 1134
Size: 11 18 1105
Fragments arranged by size:
1105 18 11
PmlI CAC'GTG
Cuts at: 0 783 1134
Size: 783 351
PsiI TTA'TAA
Cuts at: 0 356 860 1134
Size: 356 504 274
Fragments arranged by size:
504 356 274
PstI C_TGCA'G
Cuts at: 0 57 1134
Size: 57 1077
PvuII CAG'CTG
Cuts at: 0 69 1134
Size: 69 1065
SacI G_AGCT'C
Cuts at: 0 232 1134
Size: 232 902
SfcI C'TryA_G
Cuts at: 0 53 120 461 585 1134
Size: 53 67 341 124 549
Fragments arranged by size:
549 341 124 67 53
SmlI C'TyrA_G
Cuts at: 0 172 799 1134
Size: 172 627 335
Fragments arranged by size:
627 335 172
StyI C'CwwG_G
Cuts at: 0 196 1134
Size: 196 938
TatI w'GTAC_w
Cuts at: 0 1113 1134
Size: 1113 21
XmnI GAAnn'nnTTC
Cuts at: 0 499 751 835 1134
Size: 499 252 84 299
Fragments arranged by size:
499 299 252 84
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI BaeI BanII BclI
BfrBI BpmI BpuEI BsaI BsaAI BseRI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BsrGI BssSI BstAPI BtgI BtsI
CspCI EcoICRI Eco57MI EcoNI EcoRI Hin4I HindIII MmeI
MslI MspA1I NcoI NdeI NsiI PflMI PfoI PmlI
PsiI PstI PvuII SacI SfcI SmlI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI