(Circular) (Six-base) MAPSORT of: pFN21AE1025.seq Check: 2877 from: 1 to: 7219 pFN21AE1025 7219 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3245 3245 Size: 7219 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5029 278 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2293 3471 4562 1937 Size: 356 1178 1091 4594 Fragments arranged by size: 4594 1178 1091 356 Acc65I G'GTAC_C Cuts at: 4540 4540 Size: 7219 AclI AA'CG_TT Cuts at: 1563 3266 5346 5719 1563 Size: 1703 2080 373 3063 Fragments arranged by size: 3063 2080 1703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2151 3987 4413 5282 6666 6934 1632 Size: 488 31 1836 426 869 1384 268 1917 Fragments arranged by size: 1917 1836 1384 869 488 426 268 31 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7022 AflIII A'CryG_T Cuts at: 1203 2963 6118 7095 1203 Size: 1760 3155 977 1327 Fragments arranged by size: 3155 1760 1327 977 AgeI A'CCGG_T Cuts at: 6050 6050 Size: 7219 AhdI GACnn_n'nnGTC Cuts at: 5948 5948 Size: 7219 AleI CACnn'nnGTG Cuts at: 1166 3894 4324 1166 Size: 2728 430 4061 Fragments arranged by size: 4061 2728 430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7219 AlwNI CAG_nnn'CTG Cuts at: 2645 2888 3305 4962 6534 2645 Size: 243 417 1657 1572 3330 Fragments arranged by size: 3330 1657 1572 417 243 ApaLI G'TGCA_C Cuts at: 5276 6432 5276 Size: 1156 6063 ApoI r'AATT_y Cuts at: 1493 2196 2612 3241 3422 3808 3881 4124 4193 Size: 703 416 629 181 386 73 243 69 Cuts at: 4193 4312 4528 4754 4906 7015 1493 Size: 119 216 226 152 2109 1697 Fragments arranged by size: 2109 1697 703 629 416 386 243 226 216 181 152 119 73 69 AseI AT'TA_AT Cuts at: 160 1032 5773 160 Size: 872 4741 1606 Fragments arranged by size: 4741 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 AvaI C'yCGr_G Cuts at: 1870 1943 4544 1870 Size: 73 2601 4545 Fragments arranged by size: 4545 2601 73 BamHI G'GATC_C Cuts at: 3797 4549 4916 3797 Size: 752 367 6100 Fragments arranged by size: 6100 752 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2778 4540 5995 618 Size: 325 217 627 392 599 1762 1455 1842 Fragments arranged by size: 1842 1762 1455 627 599 392 325 217 BanII G_rGCy'C Cuts at: 1391 2821 4538 1391 Size: 1430 1717 4072 Fragments arranged by size: 4072 1717 1430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5410 5444 1903 Size: 34 50 34 3389 34 3678 Fragments arranged by size: 3678 3389 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5111 6321 5111 Size: 1210 6009 BclI T'GATC_A Cuts at: 1548 1548 Size: 7219 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5830 136 Size: 107 122 71 631 605 4158 1525 Fragments arranged by size: 4158 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2131 2462 7214 2131 Size: 331 4752 2136 Fragments arranged by size: 4752 2136 331 BlpI GC'TnA_GC Cuts at: 4636 4636 Size: 7219 Bme1580I G_kGCm'C Cuts at: 1790 2402 5280 6436 1790 Size: 612 2878 1156 2573 Fragments arranged by size: 2878 2573 1156 612 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3107 4950 5908 Size: 416 53 83 399 24 1690 1843 958 Cuts at: 5908 442 Size: 1753 Fragments arranged by size: 1843 1753 1690 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7219 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 2898 3088 3270 3651 5879 1239 Size: 1413 246 190 182 381 2228 2579 Fragments arranged by size: 2579 2228 1413 381 246 190 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 5344 6209 6507 4102 Size: 1242 865 298 4814 Fragments arranged by size: 4814 1242 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5882 882 Size: 617 4383 2219 Fragments arranged by size: 4383 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5010 493 Size: 711 3806 2702 Fragments arranged by size: 3806 2702 711 BsaBI GATnn'nnATC Cuts at: 2130 2501 7213 2130 Size: 371 4712 2136 Fragments arranged by size: 4712 2136 371 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5026 5408 6043 275 Size: 53 83 186 936 3493 382 635 1451 Fragments arranged by size: 3493 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5652 6050 6324 6471 6882 5652 Size: 398 274 147 411 5989 Fragments arranged by size: 5989 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6994 Fragments arranged by size: 6994 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7219 BseYI C'CCAG_C Cuts at: 6422 6918 6422 Size: 496 6723 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6646 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4669 5430 6458 1540 Size: 275 186 2668 761 1028 2301 Fragments arranged by size: 2668 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3118 5280 5365 6436 3118 Size: 2162 85 1071 3901 Fragments arranged by size: 3901 2162 1071 85 BsmI GAATG_Cn' Cuts at: 2419 3171 4730 4823 2419 Size: 752 1559 93 4815 Fragments arranged by size: 4815 1559 752 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4061 4061 Size: 7219 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2402 2821 3118 4538 5280 5365 6436 Size: 399 612 419 297 1420 742 85 1071 Cuts at: 6436 1391 Size: 2174 Fragments arranged by size: 2174 1420 1071 742 612 419 399 297 85 BspEI T'CCGG_A Cuts at: 6882 6882 Size: 7219 BspHI T'CATG_A Cuts at: 5108 6838 5108 Size: 1730 5489 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3245 4574 844 Size: 2401 1329 3489 Fragments arranged by size: 3489 2401 1329 BsrBI CCG'CTC Cuts at: 1394 1823 5106 1394 Size: 429 3283 3507 Fragments arranged by size: 3507 3283 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3144 3165 3406 3795 5714 5888 65 Size: 1183 1896 21 241 389 1919 174 1396 Fragments arranged by size: 1919 1896 1396 1183 389 241 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 5863 6050 1700 Size: 110 4053 187 2869 Fragments arranged by size: 4053 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3706 96 Size: 3610 3609 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7219 BssSI C'ACGA_G Cuts at: 5279 6291 5279 Size: 1012 6207 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7219 BstBI TT'CG_AA Cuts at: 6023 6023 Size: 7219 BstEII G'GTnAC_C Cuts at: 1184 4342 1184 Size: 3158 4061 BstXI CCAn_nnnn'nTGG Cuts at: 3619 3790 3619 Size: 171 7048 BstYI r'GATC_y Cuts at: 1972 2131 2462 2641 3797 4549 4916 5301 5318 Size: 159 331 179 1156 752 367 385 17 Cuts at: 5318 6770 6856 6868 7214 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1156 752 385 367 346 331 179 159 86 17 12 BstZ17I GTA'TAC Cuts at: 2294 2294 Size: 7219 Bsu36I CC'TnA_GG Cuts at: 4287 4287 Size: 7219 BtgI C'CryG_G Cuts at: 513 1065 3618 3666 513 Size: 552 2553 48 4066 Fragments arranged by size: 4066 2553 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4458 487 Size: 683 56 3232 3248 Fragments arranged by size: 3248 3232 683 56 BtsI GCAGTG_nn' Cuts at: 3306 3928 4735 5529 5549 3306 Size: 622 807 794 20 4976 Fragments arranged by size: 4976 807 794 622 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7219 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7184 DraI TTT'AAA Cuts at: 4523 4523 Size: 7219 DraIII CAC_nnn'GTG Cuts at: 2915 3990 2915 Size: 1075 6144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6226 809 Size: 507 4910 1802 Fragments arranged by size: 4910 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3246 3609 4666 Size: 54 1425 50 275 18 1416 363 1057 Cuts at: 4666 5555 7184 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1416 1057 889 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4666 1537 Size: 275 2854 4090 Fragments arranged by size: 4090 2854 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2350 5149 1353 Size: 997 2799 3423 Fragments arranged by size: 3423 2799 997 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5806 6180 6326 387 Size: 1064 138 4217 374 146 1280 Fragments arranged by size: 4217 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2151 2652 2898 3088 3270 3651 Size: 393 488 31 501 246 190 182 381 Cuts at: 3651 3987 4413 5282 5879 6666 6934 1239 Size: 336 426 869 597 787 268 1524 Fragments arranged by size: 1524 869 787 597 501 488 426 393 381 336 268 246 190 182 31 EcoRI G'AATT_C Cuts at: 3808 4528 4906 3808 Size: 720 378 6121 Fragments arranged by size: 6121 720 378 FspI TGC'GCA Cuts at: 5725 5725 Size: 7219 HaeII r_GCGC'y Cuts at: 1678 6366 1678 Size: 4688 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2092 2123 2124 Size: 32 135 32 485 32 3 31 1 Cuts at: 2124 2155 2306 2338 2440 2472 2938 2970 3187 Size: 31 151 32 102 32 466 32 217 Cuts at: 3187 3219 3464 3496 3851 3883 4121 4153 4160 Size: 32 245 32 355 32 238 32 7 Cuts at: 4160 4192 5860 5892 5934 5966 1373 Size: 32 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 485 466 355 245 238 217 151 135 102 42 32 32 32 32 32 32 32 32 32 32 32 32 32 31 31 7 3 1 HincII GTy'rAC Cuts at: 669 1657 1938 4276 4563 4809 669 Size: 988 281 2338 287 246 3079 Fragments arranged by size: 3079 2338 988 287 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7219 HpaI GTT'AAC Cuts at: 4276 4809 4276 Size: 533 6686 KpnI G_GTAC'C Cuts at: 4544 4544 Size: 7219 MfeI C'AATT_G Cuts at: 4818 4818 Size: 7219 MluI A'CGCG_T Cuts at: 7095 7095 Size: 7219 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3103 3669 6333 6517 1305 Size: 1798 566 2664 184 2007 Fragments arranged by size: 2664 2007 1798 566 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3248 10 Size: 54 1425 343 1416 3981 Fragments arranged by size: 3981 1425 1416 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2698 3040 3176 3894 4324 4337 5177 Size: 648 1532 342 136 718 430 13 840 Cuts at: 5177 5536 5695 518 Size: 359 159 2042 Fragments arranged by size: 2042 1532 840 718 648 430 359 342 159 136 13 MspA1I CmG'CkG Cuts at: 1606 2709 2888 3467 4635 5312 6460 6705 1606 Size: 1103 179 579 1168 677 1148 245 2120 Fragments arranged by size: 2120 1168 1148 1103 677 579 245 179 NcoI C'CATG_G Cuts at: 513 1065 3618 3666 513 Size: 552 2553 48 4066 Fragments arranged by size: 4066 2553 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 7219 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7219 NotI GC'GGCC_GC Cuts at: 4666 4666 Size: 7219 NspI r_CATG'y Cuts at: 1132 1229 2967 4577 6122 1132 Size: 97 1738 1610 1545 2229 Fragments arranged by size: 2229 1738 1610 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7219 PciI A'CATG_T Cuts at: 2963 6118 2963 Size: 3155 4064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2675 3990 1108 Size: 1567 1315 4337 Fragments arranged by size: 4337 1567 1315 PmeI GTTT'AAAC Cuts at: 4523 4523 Size: 7219 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5288 5320 6801 6833 5288 Size: 32 1481 32 5674 Fragments arranged by size: 5674 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7219 PsiI TTA'TAA Cuts at: 4789 4789 Size: 7219 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4225 4257 4225 Size: 32 7187 PstI C_TGCA'G Cuts at: 830 3301 4571 830 Size: 2471 1270 3478 Fragments arranged by size: 3478 2471 1270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7219 PvuII CAG'CTG Cuts at: 2709 2888 3467 2709 Size: 179 579 6461 Fragments arranged by size: 6461 579 179 SalI G'TCGA_C Cuts at: 1936 4561 1936 Size: 2625 4594 SbfI CC_TGCA'GG Cuts at: 4571 4571 Size: 7219 ScaI AGT'ACT Cuts at: 5467 7179 5467 Size: 1712 5507 SfcI C'TryA_G Cuts at: 826 1045 3297 3306 3468 4161 4567 5702 6383 Size: 219 2252 9 162 693 406 1135 681 Cuts at: 6383 6574 826 Size: 191 1471 Fragments arranged by size: 2252 1471 1135 693 681 406 219 191 162 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7219 SmaI CCC'GGG Cuts at: 1872 4546 1872 Size: 2674 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4117 5323 6224 6486 820 Size: 197 926 2174 1206 901 262 1553 Fragments arranged by size: 2174 1553 1206 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5010 493 Size: 4517 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7219 SphI G_CATG'C Cuts at: 1132 4577 1132 Size: 3445 3774 SspI AAT'ATT Cuts at: 5 52 2489 2524 3087 5143 5 Size: 47 2437 35 563 2056 2081 Fragments arranged by size: 2437 2081 2056 563 47 35 StuI AGG'CCT Cuts at: 1517 1517 Size: 7219 StyI C'CwwG_G Cuts at: 513 1065 3618 3666 4658 513 Size: 552 2553 48 992 3074 Fragments arranged by size: 3074 2553 992 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2197 5246 5263 5416 5601 6046 325 Size: 1255 617 3049 17 153 185 445 1498 Fragments arranged by size: 3049 1498 1255 617 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2309 2540 3044 Size: 274 80 33 51 1443 332 231 504 Cuts at: 3044 3694 3706 5465 7177 96 Size: 650 12 1759 1712 138 Fragments arranged by size: 1759 1712 1443 650 504 332 274 231 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2670 2702 7179 7211 2670 Size: 32 4477 32 2678 Fragments arranged by size: 4477 2678 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2069 1316 Size: 753 6466 XbaI T'CTAG_A Cuts at: 4555 4555 Size: 7219 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7219 XmaI C'CCGG_G Cuts at: 1870 4544 1870 Size: 2674 4545 XmnI GAAnn'nnTTC Cuts at: 2197 5348 2197 Size: 3151 4068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5027 276 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI