Restriction map for
pFN21AE1025
(Linear) (Six-base) MAPSORT of: KIEE1025.seq Check: 3744 from: 1 to: 2514
KIEE1025.orf
With 182 enzymes: SgfI *
July 3, 2012 16:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1240 2514
Size: 1240 1274
AccI GT'mk_AC
Cuts at: 0 288 1466 2514
Size: 288 1178 1048
Fragments arranged by size:
1178 1048 288
AclI AA'CG_TT
Cuts at: 0 1261 2514
Size: 1261 1253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 146 1982 2408 2514
Size: 115 31 1836 426 106
Fragments arranged by size:
1836 426 115 106 31
AflIII A'CryG_T
Cuts at: 0 958 2514
Size: 958 1556
AleI CACnn'nnGTG
Cuts at: 0 1889 2319 2514
Size: 1889 430 195
Fragments arranged by size:
1889 430 195
AlwNI CAG_nnn'CTG
Cuts at: 0 640 883 1300 2514
Size: 640 243 417 1214
Fragments arranged by size:
1214 640 417 243
ApoI r'AATT_y
Cuts at: 0 191 607 1236 1417 1803 1876 2119 2188
Size: 191 416 629 181 386 73 243 69
Cuts at: 2188 2307 2514
Size: 119 207
Fragments arranged by size:
629 416 386 243 207 191 181 119
73 69
BamHI G'GATC_C
Cuts at: 0 1792 2514
Size: 1792 722
BanI G'GyrC_C
Cuts at: 0 174 773 2514
Size: 174 599 1741
Fragments arranged by size:
1741 599 174
BanII G_rGCy'C
Cuts at: 0 816 2514
Size: 816 1698
BglII A'GATC_T
Cuts at: 0 126 457 2514
Size: 126 331 2057
Fragments arranged by size:
2057 331 126
Bme1580I G_kGCm'C
Cuts at: 0 397 2514
Size: 397 2117
BmrI ACTGGGnnnn_n'
Cuts at: 0 1102 2514
Size: 1102 1412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 893 1083 1265 1646 2514
Size: 647 246 190 182 381 868
Fragments arranged by size:
868 647 381 246 190 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2097 2514
Size: 2097 417
BsaBI GATnn'nnATC
Cuts at: 0 125 496 2514
Size: 125 371 2018
Fragments arranged by size:
2018 371 125
BsiHKAI G_wGCw'C
Cuts at: 0 1113 2514
Size: 1113 1401
BsmI GAATG_Cn'
Cuts at: 0 414 1166 2514
Size: 414 752 1348
Fragments arranged by size:
1348 752 414
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2056 2514
Size: 2056 458
Bsp1286I G_dGCh'C
Cuts at: 0 397 816 1113 2514
Size: 397 419 297 1401
Fragments arranged by size:
1401 419 397 297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1240 2514
Size: 1240 1274
BsrDI GCAATG_nn'
Cuts at: 0 1139 1160 1401 1790 2514
Size: 1139 21 241 389 724
Fragments arranged by size:
1139 724 389 241 21
BsrGI T'GTAC_A
Cuts at: 0 1701 2514
Size: 1701 813
BstEII G'GTnAC_C
Cuts at: 0 2337 2514
Size: 2337 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1614 1785 2514
Size: 1614 171 729
Fragments arranged by size:
1614 729 171
BstYI r'GATC_y
Cuts at: 0 126 457 636 1792 2514
Size: 126 331 179 1156 722
Fragments arranged by size:
1156 722 331 179 126
BstZ17I GTA'TAC
Cuts at: 0 289 2514
Size: 289 2225
Bsu36I CC'TnA_GG
Cuts at: 0 2282 2514
Size: 2282 232
BtgI C'CryG_G
Cuts at: 0 1613 1661 2514
Size: 1613 48 853
Fragments arranged by size:
1613 853 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2453 2514
Size: 2453 61
BtsI GCAGTG_nn'
Cuts at: 0 1301 1923 2514
Size: 1301 622 591
Fragments arranged by size:
1301 622 591
DraIII CAC_nnn'GTG
Cuts at: 0 910 1985 2514
Size: 910 1075 529
Fragments arranged by size:
1075 910 529
EaeI y'GGCC_r
Cuts at: 0 1241 1604 2514
Size: 1241 363 910
Fragments arranged by size:
1241 910 363
EarI CTCTTCn'nnn_
Cuts at: 0 345 2514
Size: 345 2169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 146 647 893 1083 1265 1646 1982
Size: 115 31 501 246 190 182 381 336
Cuts at: 1982 2408 2514
Size: 426 106
Fragments arranged by size:
501 426 381 336 246 190 182 115
106 31
EcoRI G'AATT_C
Cuts at: 0 1803 2514
Size: 1803 711
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 87 118 119 150 301 333
Size: 52 32 3 31 1 31 151 32
Cuts at: 333 435 467 933 965 1182 1214 1459 1491
Size: 102 32 466 32 217 32 245 32
Cuts at: 1491 1846 1878 2116 2148 2155 2187 2514
Size: 355 32 238 32 7 32 327
Fragments arranged by size:
466 355 327 245 238 217 151 102
52 32 32 32 32 32 32 32
32 32 31 31 7 3 1
HincII GTy'rAC
Cuts at: 0 2271 2514
Size: 2271 243
HpaI GTT'AAC
Cuts at: 0 2271 2514
Size: 2271 243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1098 1664 2514
Size: 1098 566 850
Fragments arranged by size:
1098 850 566
MscI TGG'CCA
Cuts at: 0 1243 2514
Size: 1243 1271
MslI CAynn'nnrTG
Cuts at: 0 693 1035 1171 1889 2319 2332 2514
Size: 693 342 136 718 430 13 182
Fragments arranged by size:
718 693 430 342 182 136 13
MspA1I CmG'CkG
Cuts at: 0 704 883 1462 2514
Size: 704 179 579 1052
Fragments arranged by size:
1052 704 579 179
NcoI C'CATG_G
Cuts at: 0 1613 1661 2514
Size: 1613 48 853
Fragments arranged by size:
1613 853 48
NspI r_CATG'y
Cuts at: 0 962 2514
Size: 962 1552
PciI A'CATG_T
Cuts at: 0 958 2514
Size: 958 1556
PflMI CCAn_nnn'nTGG
Cuts at: 0 670 1985 2514
Size: 670 1315 529
Fragments arranged by size:
1315 670 529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2220 2252 2514
Size: 2220 32 262
Fragments arranged by size:
2220 262 32
PstI C_TGCA'G
Cuts at: 0 1296 2514
Size: 1296 1218
PvuII CAG'CTG
Cuts at: 0 704 883 1462 2514
Size: 704 179 579 1052
Fragments arranged by size:
1052 704 579 179
SfcI C'TryA_G
Cuts at: 0 1292 1301 1463 2156 2514
Size: 1292 9 162 693 358
Fragments arranged by size:
1292 693 358 162 9
SmlI C'TyrA_G
Cuts at: 0 2112 2514
Size: 2112 402
SspI AAT'ATT
Cuts at: 0 484 519 1082 2514
Size: 484 35 563 1432
Fragments arranged by size:
1432 563 484 35
StyI C'CwwG_G
Cuts at: 0 1613 1661 2514
Size: 1613 48 853
Fragments arranged by size:
1613 853 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 2514
Size: 192 2322
TatI w'GTAC_w
Cuts at: 0 304 535 1039 1689 1701 2514
Size: 304 231 504 650 12 813
Fragments arranged by size:
813 650 504 304 231 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 665 697 2514
Size: 665 32 1817
Fragments arranged by size:
1817 665 32
Tth111I GACn'n_nGTC
Cuts at: 0 64 2514
Size: 64 2450
XmnI GAAnn'nnTTC
Cuts at: 0 192 2514
Size: 192 2322
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AlwNI ApoI
BamHI BanI BanII BglII Bme1580I BmrI BpmI BpuEI
BsaBI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI Eco57MI EcoRI Hin4I HincII HpaI
MmeI MscI MslI MspA1I NcoI NspI PciI PflMI
PsrI PstI PvuII SfcI SmlI SspI StyI TaqII
TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BspEI
BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI XbaI XcmI XhoI XmaI ZraI