Restriction map for
pFN21AB5521
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspEI BspMI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NciI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
StuI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AflIII 132 ACATGT A^C[GA][CT]GT
AocI 928 CCTCAGG CC^T[ACGT]AGG
Aor51HI 787 AGCGCT AGC^GCT
Asp700I 939 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 709 GAGCTC G[AT]GC[AT]^C
AvrII 265 CCTAGG C^CTAGG
BalI 531 TGGCCA TGG^CCA
BamHI 834 GGATCC G^GATCC
BbsI 552 GAAGAC GAAGAC(2/6)
BbvCI 790 GCTGAGG CCTCAGC(-5/-2)
BfaI 266 CTAG C^TAG
BlnI 265 CCTAGG C^CTAGG
BpmI 186 CTGGAG CTGGAG(16/14)
BpuAI 552 GAAGAC GAAGAC(2/6)
BsaI 191 GAGACC GGTCTC(1/5)
BsiEI 1027 CGACCG CG[GA][CT]^CG
BsiHKAI 709 GAGCTC G[AT]GC[AT]^C
BspDI 453 ATCGAT AT^CGAT
BspHI 111 TCATGA T^CATGA
BspLU11I 132 ACATGT A^CATGT
BsrBI 934 GAGCGG CCGCTC(-3/-3)
BsrFI 663 GCCGGC [GA]^CCGG[CT]
BssSI 641 CTCGTG CACGAG(-5/-1)
BstXI 585 CCAGTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 928 CCTCAGG CC^T[ACGT]AGG
CfoI 788 GCGC GCG^C
Cfr10I 663 GCCGGC [GA]^CCGG[CT]
ClaI 453 ATCGAT AT^CGAT
DraII 72 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 560 CCATGG C^C[GA][CT]GG
EaeI 531 TGGCCA [CT]^GGCC[GA]
Eco47III 787 AGCGCT AGC^GCT
Eco81I 928 CCTCAGG CC^T[ACGT]AGG
EcoNI 52 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 72 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 894 GAATTC G^AATTC
HaeII 787 AGCGCT [GA]GCGC^[CT]
HapII 664 CCGG C^CGG
HhaI 788 GCGC GCG^C
HinP1I 788 GCGC G^CGC
HpaII 664 CCGG C^CGG
HphI 595 GGTGA GGTGA(8/7)
MaeI 266 CTAG C^TAG
MluNI 531 TGGCCA TGG^CCA
MscI 531 TGGCCA TGG^CCA
MseI 521 TTAA T^TAA
MspA1I 1282 CAGCGG C[AC]G^C[GT]G
MspI 664 CCGG C^CGG
NaeI 663 GCCGGC GCC^GGC
NcoI 560 CCATGG C^CATGG
NgoMIV 663 GCCGGC G^CCGGC
PflMI 179 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 72 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1165 CTGCAG CTGCA^G
RcaI 111 TCATGA T^CATGA
SacI 709 GAGCTC GAGCT^C
SapI 495 GAAGAGC GCTCTTC(1/4)
SbfI 1164 CCTGCAGG CCTGCA^GG
SfcI 1165 CTGCAG C^T[GA][CT]AG
Sse8387I 1164 CCTGCAGG CCTGCA^GG
SspI 335 AATATT AAT^ATT
Tru9I 521 TTAA T^TAA
Van91I 179 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 939 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 1144 GTAC GT^AC
1269 GTAC
AvaII 73 GGACC G^G[AT]CC
800 GGACC
BseRI 706 GAGGAG GAGGAG(10/8)
793 GAGGAG
BsmAI 191 GAGAC GTCTC(1/5)
444 GTCTC
BsrDI 1085 CATTGC GCAATG(2/0)
205 GCAATG
BssKI 52 CCTGG ^CC[ACGT]GG
468 CCTGG
BstNI 52 CCTGG CC^[AT]GG
468 CCTGG
Cac8I 6 GCGGGC GC[ACGT]^[ACGT]GC
663 GCCGGC
EcoRII 52 CCTGG ^CC[AT]GG
468 CCTGG
FauI 6 GCGGG CCCGC(4/6)
159 GCGGG
MaeII 763 ACGT A^CGT
1207 ACGT
MvaI 52 CCTGG CC^[AT]GG
468 CCTGG
NdeI 983 CATATG CA^TATG
1250 CATATG
RsaI 1144 GTAC GT^AC
1269 GTAC
ScrFI 52 CCTGG CC^[ACGT]GG
468 CCTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BstF5I BstYI
Cfr13I DdeI DpnII EarI EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MwoI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
SfaNI SmlI StyI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XhoII