Restriction map for
pFN21AB5521
(Linear) (Six-base) MAPSORT of: KIBB5521.seq Check: 4971 from: 1 to: 1344
KIBB5521.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 925 1344
Size: 738 187 419
Fragments arranged by size:
738 419 187
AfeI AGC'GCT
Cuts at: 0 789 1344
Size: 789 555
AflIII A'CryG_T
Cuts at: 0 132 1344
Size: 132 1212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 1344
Size: 508 32 804
Fragments arranged by size:
804 508 32
ApoI r'AATT_y
Cuts at: 0 601 894 917 1344
Size: 601 293 23 427
Fragments arranged by size:
601 427 293 23
AvrII C'CTAG_G
Cuts at: 0 265 1344
Size: 265 1079
BamHI G'GATC_C
Cuts at: 0 834 1344
Size: 834 510
BanII G_rGCy'C
Cuts at: 0 508 713 1343 1344
Size: 508 205 630 1
Fragments arranged by size:
630 508 205 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 559 1344
Size: 559 785
BbvCI CC'TCA_GC
Cuts at: 0 791 1344
Size: 791 553
BmgBI CAC'GTC
Cuts at: 0 764 1344
Size: 764 580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1344
Size: 1098 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1344
Size: 207 1137
Bpu10I CC'TnA_GC
Cuts at: 0 791 1344
Size: 791 553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 397 522 1338 1344
Size: 53 344 125 816 6
Fragments arranged by size:
816 344 125 53 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 185 1344
Size: 185 1159
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 808 1344
Size: 721 87 536
Fragments arranged by size:
721 536 87
BseYI C'CCAG_C
Cuts at: 0 542 784 1308 1344
Size: 542 242 524 36
Fragments arranged by size:
542 524 242 36
BsiEI CG_ry'CG
Cuts at: 0 1030 1344
Size: 1030 314
BsiHKAI G_wGCw'C
Cuts at: 0 713 1344
Size: 713 631
Bsp1286I G_dGCh'C
Cuts at: 0 508 713 1343 1344
Size: 508 205 630 1
Fragments arranged by size:
630 508 205 1
BspHI T'CATG_A
Cuts at: 0 111 1344
Size: 111 1233
BsrBI CCG'CTC
Cuts at: 0 936 1344
Size: 936 408
BsrDI GCAATG_nn'
Cuts at: 0 212 1084 1344
Size: 212 872 260
Fragments arranged by size:
872 260 212
BsrFI r'CCGG_y
Cuts at: 0 663 1344
Size: 663 681
BssSI C'ACGA_G
Cuts at: 0 641 1344
Size: 641 703
BstXI CCAn_nnnn'nTGG
Cuts at: 0 592 1344
Size: 592 752
BstYI r'GATC_y
Cuts at: 0 381 834 953 1344
Size: 381 453 119 391
Fragments arranged by size:
453 391 381 119
Bsu36I CC'TnA_GG
Cuts at: 0 929 1344
Size: 929 415
BtgI C'CryG_G
Cuts at: 0 560 1344
Size: 560 784
BtsI GCAGTG_nn'
Cuts at: 0 13 318 887 1344
Size: 13 305 569 457
Fragments arranged by size:
569 457 305 13
ClaI AT'CG_AT
Cuts at: 0 454 1344
Size: 454 890
EaeI y'GGCC_r
Cuts at: 0 531 1344
Size: 531 813
EarI CTCTTCn'nnn_
Cuts at: 0 51 490 698 901 1344
Size: 51 439 208 203 443
Fragments arranged by size:
443 439 208 203 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 423 1344
Size: 423 921
EcoICRI GAG'CTC
Cuts at: 0 711 1344
Size: 711 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 738 925 1344
Size: 207 531 187 419
Fragments arranged by size:
531 419 207 187
EcoNI CCTnn'n_nnAGG
Cuts at: 0 56 1344
Size: 56 1288
EcoO109I rG'GnC_Cy
Cuts at: 0 73 1344
Size: 73 1271
EcoRI G'AATT_C
Cuts at: 0 894 1344
Size: 894 450
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 516 1344
Size: 61 455 828
Fragments arranged by size:
828 455 61
HaeII r_GCGC'y
Cuts at: 0 791 1344
Size: 791 553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1344
Size: 1196 148
MscI TGG'CCA
Cuts at: 0 533 1344
Size: 533 811
MslI CAynn'nnrTG
Cuts at: 0 110 565 596 1107 1210 1344
Size: 110 455 31 511 103 134
Fragments arranged by size:
511 455 134 110 103 31
MspA1I CmG'CkG
Cuts at: 0 1284 1344
Size: 1284 60
NaeI GCC'GGC
Cuts at: 0 665 1344
Size: 665 679
NcoI C'CATG_G
Cuts at: 0 560 1344
Size: 560 784
NdeI CA'TA_TG
Cuts at: 0 984 1251 1344
Size: 984 267 93
Fragments arranged by size:
984 267 93
NgoMIV G'CCGG_C
Cuts at: 0 663 1344
Size: 663 681
NspI r_CATG'y
Cuts at: 0 122 136 417 1344
Size: 122 14 281 927
Fragments arranged by size:
927 281 122 14
PciI A'CATG_T
Cuts at: 0 132 1344
Size: 132 1212
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 1344
Size: 185 1159
PfoI T'CCnGG_A
Cuts at: 0 51 1344
Size: 51 1293
PpuMI rG'GwC_Cy
Cuts at: 0 73 1344
Size: 73 1271
PstI C_TGCA'G
Cuts at: 0 1169 1344
Size: 1169 175
SacI G_AGCT'C
Cuts at: 0 713 1344
Size: 713 631
SapI GCTCTTCn'nnn_
Cuts at: 0 490 1344
Size: 490 854
SbfI CC_TGCA'GG
Cuts at: 0 1169 1344
Size: 1169 175
SfcI C'TryA_G
Cuts at: 0 1165 1344
Size: 1165 179
SmlI C'TyrA_G
Cuts at: 0 68 376 501 1317 1344
Size: 68 308 125 816 27
Fragments arranged by size:
816 308 125 68 27
SspI AAT'ATT
Cuts at: 0 337 1344
Size: 337 1007
StyI C'CwwG_G
Cuts at: 0 29 265 560 1344
Size: 29 236 295 784
Fragments arranged by size:
784 295 236 29
TatI w'GTAC_w
Cuts at: 0 1143 1268 1344
Size: 1143 125 76
Fragments arranged by size:
1143 125 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 819 851 945 977 1344
Size: 819 32 94 32 367
Fragments arranged by size:
819 367 94 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 943 1344
Size: 943 401
Enzymes that do cut:
AcuI AfeI AflIII AloI ApoI AvrII BamHI BanII
BbsI BbvCI BmgBI BplI BpmI Bpu10I BpuEI BsaI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI
BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PpuMI PstI
SacI SapI SbfI SfcI SmlI SspI StyI TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BanI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI
BspMI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
CspCI DraI DraIII DrdI EagI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI