(Circular) (Six-base) MAPSORT of: pFN21AE1001.seq Check: 5195 from: 1 to: 5104 pFN21AE1001 5104 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2914 278 Size: 53 83 186 936 1378 2468 Fragments arranged by size: 2468 1378 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2447 1937 Size: 510 4594 Acc65I G'GTAC_C Cuts at: 2037 2425 2037 Size: 388 4716 AclI AA'CG_TT Cuts at: 1563 3231 3604 1563 Size: 1668 373 3063 Fragments arranged by size: 3063 1668 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2357 3167 4551 4819 1632 Size: 555 170 810 1384 268 1917 Fragments arranged by size: 1917 1384 810 555 268 170 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4907 AflIII A'CryG_T Cuts at: 1203 2193 4003 4980 1203 Size: 990 1810 977 1327 Fragments arranged by size: 1810 1327 990 977 AgeI A'CCGG_T Cuts at: 3935 3935 Size: 5104 AhdI GACnn_n'nnGTC Cuts at: 3833 3833 Size: 5104 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5104 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5104 AlwNI CAG_nnn'CTG Cuts at: 2847 4419 2847 Size: 1572 3532 ApaI G_GGCC'C Cuts at: 2143 2143 Size: 5104 ApaLI G'TGCA_C Cuts at: 3161 4317 3161 Size: 1156 3948 ApoI r'AATT_y Cuts at: 1493 2413 2639 2791 4900 1493 Size: 920 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 226 152 AseI AT'TA_AT Cuts at: 160 1032 3658 160 Size: 872 2626 1606 Fragments arranged by size: 2626 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5104 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2429 1870 Size: 73 72 414 4545 Fragments arranged by size: 4545 414 73 72 BamHI G'GATC_C Cuts at: 2434 2801 2434 Size: 367 4737 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 2425 3880 618 Size: 325 217 627 250 388 1455 1842 Fragments arranged by size: 1842 1455 627 388 325 250 217 BanII G_rGCy'C Cuts at: 1391 2061 2143 2423 1391 Size: 670 82 280 4072 Fragments arranged by size: 4072 670 280 82 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3295 3329 1903 Size: 34 50 34 1274 34 3678 Fragments arranged by size: 3678 1274 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2996 4206 2996 Size: 1210 3894 BclI T'GATC_A Cuts at: 1548 1548 Size: 5104 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3715 136 Size: 107 122 71 631 605 2043 1525 Fragments arranged by size: 2043 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5099 5099 Size: 5104 BlpI GC'TnA_GC Cuts at: 2521 2521 Size: 5104 Bme1580I G_kGCm'C Cuts at: 1790 2143 3165 4321 1790 Size: 353 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 353 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5104 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2835 3793 442 Size: 416 53 83 399 24 1418 958 1753 Fragments arranged by size: 1753 1418 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5104 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3764 1239 Size: 2525 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3229 4094 4392 2369 Size: 860 865 298 3081 Fragments arranged by size: 3081 865 860 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3767 882 Size: 617 2268 2219 Fragments arranged by size: 2268 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2895 493 Size: 711 1691 2702 Fragments arranged by size: 2702 1691 711 BsaBI GATnn'nnATC Cuts at: 2247 5098 2247 Size: 2851 2253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2911 3293 3928 275 Size: 53 83 186 936 1378 382 635 1451 Fragments arranged by size: 1451 1378 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2329 3537 3935 4209 4356 4767 2329 Size: 1208 398 274 147 411 2666 Fragments arranged by size: 2666 1208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4879 Fragments arranged by size: 4879 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2081 1186 Size: 883 12 4209 Fragments arranged by size: 4209 883 12 BseYI C'CCAG_C Cuts at: 4307 4803 4307 Size: 496 4608 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4531 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2554 3315 4343 1540 Size: 275 186 553 761 1028 2301 Fragments arranged by size: 2301 1028 761 553 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 3165 3250 4321 2061 Size: 1104 85 1071 2844 Fragments arranged by size: 2844 1104 1071 85 BsmI GAATG_Cn' Cuts at: 2615 2708 2615 Size: 93 5011 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2143 2423 3165 3250 4321 1391 Size: 399 271 82 280 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 280 271 85 82 BspEI T'CCGG_A Cuts at: 4767 4767 Size: 5104 BspHI T'CATG_A Cuts at: 2993 4723 2993 Size: 1730 3374 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2459 844 Size: 1615 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2991 1394 Size: 429 1168 3507 Fragments arranged by size: 3507 1168 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3599 3773 65 Size: 1183 2351 174 1396 Fragments arranged by size: 2351 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3748 3935 1700 Size: 110 1938 187 2869 Fragments arranged by size: 2869 1938 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5104 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5104 BssSI C'ACGA_G Cuts at: 3164 4176 3164 Size: 1012 4092 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5104 BstBI TT'CG_AA Cuts at: 3908 3908 Size: 5104 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5104 BstYI r'GATC_y Cuts at: 1972 2152 2434 2801 3186 3203 4655 4741 4753 Size: 180 282 367 385 17 1452 86 12 Cuts at: 4753 5099 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 282 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2135 2135 Size: 5104 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4365 Fragments arranged by size: 4365 683 56 BtsI GCAGTG_nn' Cuts at: 2620 3414 3434 2620 Size: 794 20 4290 Fragments arranged by size: 4290 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5104 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5069 DraI TTT'AAA Cuts at: 2408 2408 Size: 5104 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4111 809 Size: 507 2795 1802 Fragments arranged by size: 2795 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2551 3440 5069 Size: 54 1425 50 275 18 721 889 1629 Cuts at: 5069 8 Size: 43 Fragments arranged by size: 1629 1425 889 721 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2551 1537 Size: 275 739 4090 Fragments arranged by size: 4090 739 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3034 1353 Size: 1681 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 3691 4065 4211 387 Size: 1064 138 504 1598 374 146 1280 Fragments arranged by size: 1598 1280 1064 504 374 146 138 EcoICRI GAG'CTC Cuts at: 2059 2059 Size: 5104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2357 3167 3764 4551 4819 1239 Size: 393 555 170 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 555 393 268 170 EcoNI CCTnn'n_nnAGG Cuts at: 2147 2312 2363 2147 Size: 165 51 4888 Fragments arranged by size: 4888 165 51 EcoO109I rG'GnC_Cy Cuts at: 2131 2139 2140 2131 Size: 8 1 5095 Fragments arranged by size: 5095 8 1 EcoRI G'AATT_C Cuts at: 2413 2791 2413 Size: 378 4726 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 5104 FspI TGC'GCA Cuts at: 3610 3610 Size: 5104 HaeII r_GCGC'y Cuts at: 1678 4251 1678 Size: 2573 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3745 3777 3819 3851 1373 Size: 32 135 32 2173 32 42 32 2626 Fragments arranged by size: 2626 2173 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2302 2448 2694 669 Size: 988 281 364 146 246 3079 Fragments arranged by size: 3079 988 364 281 246 146 HindIII A'AGCT_T Cuts at: 748 748 Size: 5104 HpaI GTT'AAC Cuts at: 2694 2694 Size: 5104 KpnI G_GTAC'C Cuts at: 2041 2429 2041 Size: 388 4716 MfeI C'AATT_G Cuts at: 2703 2703 Size: 5104 MluI A'CGCG_T Cuts at: 4980 4980 Size: 5104 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4218 4402 1305 Size: 2913 184 2007 Fragments arranged by size: 2913 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3282 Fragments arranged by size: 3282 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3062 3421 3580 518 Size: 648 1896 359 159 2042 Fragments arranged by size: 2042 1896 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2520 3197 4345 4590 1606 Size: 914 677 1148 245 2120 Fragments arranged by size: 2120 1148 914 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5104 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5104 NotI GC'GGCC_GC Cuts at: 2551 2551 Size: 5104 NspI r_CATG'y Cuts at: 1132 1229 2197 2462 4007 1132 Size: 97 968 265 1545 2229 Fragments arranged by size: 2229 1545 968 265 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5104 PciI A'CATG_T Cuts at: 2193 4003 2193 Size: 1810 3294 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5104 PmeI GTTT'AAAC Cuts at: 2408 2408 Size: 5104 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3173 3205 4686 4718 3173 Size: 32 1481 32 3559 Fragments arranged by size: 3559 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5104 PsiI TTA'TAA Cuts at: 2674 2674 Size: 5104 PspOMI G'GGCC_C Cuts at: 2139 2139 Size: 5104 PstI C_TGCA'G Cuts at: 830 2148 2456 830 Size: 1318 308 3478 Fragments arranged by size: 3478 1318 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5104 SacI G_AGCT'C Cuts at: 2061 2061 Size: 5104 SalI G'TCGA_C Cuts at: 1936 2446 1936 Size: 510 4594 SbfI CC_TGCA'GG Cuts at: 2456 2456 Size: 5104 ScaI AGT'ACT Cuts at: 3352 5064 3352 Size: 1712 3392 SfcI C'TryA_G Cuts at: 826 1045 2144 2452 3587 4268 4459 826 Size: 219 1099 308 1135 681 191 1471 Fragments arranged by size: 1471 1135 1099 681 308 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5104 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5104 SmaI CCC'GGG Cuts at: 1872 2431 1872 Size: 559 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3208 4109 4371 820 Size: 197 926 441 824 901 262 1553 Fragments arranged by size: 1553 926 901 824 441 262 197 SnaBI TAC'GTA Cuts at: 493 2895 493 Size: 2402 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5104 SphI G_CATG'C Cuts at: 1132 2462 1132 Size: 1330 3774 SspI AAT'ATT Cuts at: 5 52 3028 5 Size: 47 2976 2081 Fragments arranged by size: 2976 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5104 StyI C'CwwG_G Cuts at: 513 1065 2320 2543 513 Size: 552 1255 223 3074 Fragments arranged by size: 3074 1255 552 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3131 3148 3301 3486 3931 325 Size: 1255 1551 17 153 185 445 1498 Fragments arranged by size: 1551 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3350 5062 96 Size: 274 80 33 51 1443 1373 1712 138 Fragments arranged by size: 1712 1443 1373 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5064 5096 5064 Size: 32 5072 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5104 XbaI T'CTAG_A Cuts at: 2440 2440 Size: 5104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5104 XmaI C'CCGG_G Cuts at: 1870 2429 1870 Size: 559 4545 XmnI GAAnn'nnTTC Cuts at: 3233 3233 Size: 5104 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2912 276 Size: 53 83 186 936 1378 2468 Fragments arranged by size: 2468 1378 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI