Restriction map for
pFN21AE1006
(Circular) (Six-base) MAPSORT of: pFN21AE1006.seq Check: 1255 from: 1 to: 5266
pFN21AE1006 5266 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3076 278
Size: 53 83 186 936 1540 2468
Fragments arranged by size:
2468 1540 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2609 1937
Size: 672 4594
Acc65I G'GTAC_C
Cuts at: 2587 2587
Size: 5266
AclI AA'CG_TT
Cuts at: 1563 3393 3766 1563
Size: 1830 373 3063
Fragments arranged by size:
3063 1830 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3329 4713 4981 1632
Size: 1697 1384 268 1917
Fragments arranged by size:
1917 1697 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5069
AflIII A'CryG_T
Cuts at: 1203 2504 4165 5142 1203
Size: 1301 1661 977 1327
Fragments arranged by size:
1661 1327 1301 977
AgeI A'CCGG_T
Cuts at: 4097 4097
Size: 5266
AhdI GACnn_n'nnGTC
Cuts at: 3995 3995
Size: 5266
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5266
AlwNI CAG_nnn'CTG
Cuts at: 3009 4581 3009
Size: 1572 3694
ApaI G_GGCC'C
Cuts at: 2037 2491 2037
Size: 454 4812
ApaLI G'TGCA_C
Cuts at: 3323 4479 3323
Size: 1156 4110
ApoI r'AATT_y
Cuts at: 1493 2447 2575 2801 2953 5062 1493
Size: 954 128 226 152 2109 1697
Fragments arranged by size:
2109 1697 954 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 3820 160
Size: 872 2788 1606
Fragments arranged by size:
2788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5266
AvaI C'yCGr_G
Cuts at: 1870 1943 2329 2591 1870
Size: 73 386 262 4545
Fragments arranged by size:
4545 386 262 73
BamHI G'GATC_C
Cuts at: 2236 2596 2963 2236
Size: 360 367 4539
Fragments arranged by size:
4539 367 360
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2587 4042 618
Size: 325 217 627 800 1455 1842
Fragments arranged by size:
1842 1455 800 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2330 2412 2484 2491 2585 1391
Size: 646 293 82 72 7 94 4072
Fragments arranged by size:
4072 646 293 94 82 72 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4295
BbvCI CC'TCA_GC
Cuts at: 2018 2417 2018
Size: 399 4867
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3457 3491 1903
Size: 34 50 34 1436 34 3678
Fragments arranged by size:
3678 1436 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3158 4368 3158
Size: 1210 4056
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5266
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2495 3877
Size: 107 122 71 631 605 427 396 1382
Cuts at: 3877 136
Size: 1525
Fragments arranged by size:
1525 1382 631 605 427 396 122 107
71
BglII A'GATC_T
Cuts at: 5261 5261
Size: 5266
BlpI GC'TnA_GC
Cuts at: 2683 2683
Size: 5266
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2348 2491 2538 3327 4483 1790
Size: 247 311 143 47 789 1156 2573
Fragments arranged by size:
2573 1156 789 311 247 143 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2997 3955 442
Size: 416 53 83 399 24 1580 958 1753
Fragments arranged by size:
1753 1580 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 3926 1239
Size: 1212 1475 2579
Fragments arranged by size:
2579 1475 1212
Bpu10I CC'TnA_GC
Cuts at: 2018 2417 2483 2018
Size: 399 66 4801
Fragments arranged by size:
4801 399 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 3391 4256 4554 2059
Size: 1332 865 298 2771
Fragments arranged by size:
2771 1332 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2427 3929 882
Size: 617 928 1502 2219
Fragments arranged by size:
2219 1502 928 617
BsaAI yAC'GTr
Cuts at: 493 1204 2505 3057 493
Size: 711 1301 552 2702
Fragments arranged by size:
2702 1301 711 552
BsaBI GATnn'nnATC
Cuts at: 5260 5260
Size: 5266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2129 2459 3073 3455
Size: 53 83 186 936 596 330 614 382
Cuts at: 3455 4090 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 614 596 382 330 186
83 53
BsaWI w'CCGG_w
Cuts at: 3699 4097 4371 4518 4929 3699
Size: 398 274 147 411 4036
Fragments arranged by size:
4036 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5041
Fragments arranged by size:
5041 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5266
BseYI C'CCAG_C
Cuts at: 2113 2225 4469 4965 2113
Size: 112 2244 496 2414
Fragments arranged by size:
2414 2244 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4693
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2716 3477 4505 1540
Size: 275 186 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 275 186
BsiHKAI G_wGCw'C
Cuts at: 2484 3327 3412 4483 2484
Size: 843 85 1071 3267
Fragments arranged by size:
3267 1071 843 85
BsmI GAATG_Cn'
Cuts at: 2777 2870 2777
Size: 93 5173
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2330 2348 2412 2484 2491 2538
Size: 399 247 293 18 64 72 7 47
Cuts at: 2538 2585 3327 3412 4483 1391
Size: 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 293 247 85 72
64 47 47 18 7
BspEI T'CCGG_A
Cuts at: 4929 4929
Size: 5266
BspHI T'CATG_A
Cuts at: 3155 4885 3155
Size: 1730 3536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2621 844
Size: 1777 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3153 1394
Size: 429 1330 3507
Fragments arranged by size:
3507 1330 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2293 3761 3935 65
Size: 1183 1045 1468 174 1396
Fragments arranged by size:
1468 1396 1183 1045 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3910 4097 1700
Size: 110 198 1902 187 2869
Fragments arranged by size:
2869 1902 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5266
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5266
BssSI C'ACGA_G
Cuts at: 3326 4338 3326
Size: 1012 4254
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 3983
BstBI TT'CG_AA
Cuts at: 4070 4070
Size: 5266
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5266
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5266
BstYI r'GATC_y
Cuts at: 1972 2236 2596 2963 3348 3365 4817 4903 4915
Size: 264 360 367 385 17 1452 86 12
Cuts at: 4915 5261 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 360 346 264 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2440 513
Size: 552 1375 3339
Fragments arranged by size:
3339 1375 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4527
Fragments arranged by size:
4527 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 2782 3576 3596 2305
Size: 477 794 20 3975
Fragments arranged by size:
3975 794 477 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5231
DraI TTT'AAA
Cuts at: 2570 2570
Size: 5266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4273 809
Size: 507 2957 1802
Fragments arranged by size:
2957 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2713 3602 5231
Size: 54 1425 50 275 18 883 889 1629
Cuts at: 5231 8
Size: 43
Fragments arranged by size:
1629 1425 889 883 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2713 1537
Size: 275 901 4090
Fragments arranged by size:
4090 901 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3196 1353
Size: 1843 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3853 4227 4373 387
Size: 1064 138 2264 374 146 1280
Fragments arranged by size:
2264 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2482
Size: 5266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 3329 3926 4713 4981 1239
Size: 393 819 878 597 787 268 1524
Fragments arranged by size:
1524 878 819 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2034 2333 2487 2488 2034
Size: 299 154 1 4812
Fragments arranged by size:
4812 299 154 1
EcoRI G'AATT_C
Cuts at: 2575 2953 2575
Size: 378 4888
FspI TGC'GCA
Cuts at: 3772 3772
Size: 5266
HaeII r_GCGC'y
Cuts at: 1678 4413 1678
Size: 2735 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 3907 3939 3981
Size: 32 135 32 968 32 1335 32 42
Cuts at: 3981 4013 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 968 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2610 2856 669
Size: 988 281 337 335 246 3079
Fragments arranged by size:
3079 988 337 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5266
HpaI GTT'AAC
Cuts at: 2856 2856
Size: 5266
KpnI G_GTAC'C
Cuts at: 2591 2591
Size: 5266
MfeI C'AATT_G
Cuts at: 2865 2865
Size: 5266
MluI A'CGCG_T
Cuts at: 5142 5142
Size: 5266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4380 4564 1305
Size: 3075 184 2007
Fragments arranged by size:
3075 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3444
Fragments arranged by size:
3444 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2361 3224 3583 3742 518
Size: 648 843 352 863 359 159 2042
Fragments arranged by size:
2042 863 843 648 359 352 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2554 2682 3359 4507 4752 1606
Size: 471 477 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 477 471 245 128
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4714
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5266
NotI GC'GGCC_GC
Cuts at: 2713 2713
Size: 5266
NspI r_CATG'y
Cuts at: 1132 1229 2084 2624 4169 1132
Size: 97 855 540 1545 2229
Fragments arranged by size:
2229 1545 855 540 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5266
PciI A'CATG_T
Cuts at: 4165 4165
Size: 5266
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5266
PmeI GTTT'AAAC
Cuts at: 2570 2570
Size: 5266
PmlI CAC'GTG
Cuts at: 2505 2505
Size: 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 4848 4880 3335
Size: 32 1481 32 3721
Fragments arranged by size:
3721 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5266
PsiI TTA'TAA
Cuts at: 2836 2836
Size: 5266
PspOMI G'GGCC_C
Cuts at: 2033 2487 2033
Size: 454 4812
PstI C_TGCA'G
Cuts at: 830 2618 830
Size: 1788 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5266
PvuII CAG'CTG
Cuts at: 2554 2554
Size: 5266
SacI G_AGCT'C
Cuts at: 2484 2484
Size: 5266
SalI G'TCGA_C
Cuts at: 1936 2608 1936
Size: 672 4594
SbfI CC_TGCA'GG
Cuts at: 2618 2618
Size: 5266
ScaI AGT'ACT
Cuts at: 3514 5226 3514
Size: 1712 3554
SfcI C'TryA_G
Cuts at: 826 1045 2614 3749 4430 4621 826
Size: 219 1569 1135 681 191 1471
Fragments arranged by size:
1569 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5266
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5266
SmaI CCC'GGG
Cuts at: 1872 2593 1872
Size: 721 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 3370 4271 4533 820
Size: 197 926 95 1332 901 262 1553
Fragments arranged by size:
1553 1332 926 901 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3057 493
Size: 2564 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5266
SphI G_CATG'C
Cuts at: 1132 2624 1132
Size: 1492 3774
SspI AAT'ATT
Cuts at: 5 52 3190 5
Size: 47 3138 2081
Fragments arranged by size:
3138 2081 47
StuI AGG'CCT
Cuts at: 1517 2352 1517
Size: 835 4431
StyI C'CwwG_G
Cuts at: 513 1065 2199 2340 2705 513
Size: 552 1134 141 365 3074
Fragments arranged by size:
3074 1134 552 365 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3293 3310 3463 3648 4093 325
Size: 1255 570 1143 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1143 570 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3512 5224 96
Size: 274 80 33 51 1443 1535 1712 138
Fragments arranged by size:
1712 1535 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5226 5258 5226
Size: 32 5234
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5266
XbaI T'CTAG_A
Cuts at: 2602 2602
Size: 5266
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5266
XmaI C'CCGG_G
Cuts at: 1870 2591 1870
Size: 721 4545
XmnI GAAnn'nnTTC
Cuts at: 3395 3395
Size: 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3074 276
Size: 53 83 186 936 1540 2468
Fragments arranged by size:
2468 1540 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI