Restriction map for
pFN21AB5473
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HinfI HpaI KasI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TfiI
Tsp509I Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 55 GGTACC G^GTACC
AcyI 196 GACGCC G[GA]^CG[CT]C
AflIII 347 ACATGT A^C[GA][CT]GT
Alw44I 673 GTGCAC G^TGCAC
AocI 458 CCTCAGG CC^T[ACGT]AGG
ApaLI 673 GTGCAC G^TGCAC
Asp718I 55 GGTACC G^GTACC
AvaI 641 CTCGAG C^[CT]CG[GA]G
AvaII 15 GGTCC G^G[AT]CC
BanI 55 GGTACC G^G[CT][GA]CC
BbsI 726 GAAGAC GAAGAC(2/6)
BbvCI 658 GCTGAGG CCTCAGC(-5/-2)
BpuAI 726 GAAGAC GAAGAC(2/6)
BsaAI 676 CACGTA [CT]AC^GT[GA]
BsaHI 196 GACGCC G[GA]^CG[CT]C
BsaI 693 GAGACC GGTCTC(1/5)
BseRI 148 GAGGAG GAGGAG(10/8)
BsgI 323 CTGCAC GTGCAG(16/14)
BsmAI 693 GAGAC GTCTC(1/5)
BsoBI 641 CTCGAG C^[CT]CG[GA]G
Bsp1407I 446 TGTACA T^GTACA
BspLU11I 347 ACATGT A^CATGT
BspMI 767 ACCTGC ACCTGC(4/8)
BsrGI 446 TGTACA T^GTACA
BsrI 650 CCAGT ACTGG(1/-1)
BstAPI 596 GCATTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 201 CCACCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 458 CCTCAGG CC^T[ACGT]AGG
CfoI 375 GCGC GCG^C
DsaI 275 CCGTGG C^C[GA][CT]GG
Eco81I 458 CCTCAGG CC^T[ACGT]AGG
EcoRV 46 GATATC GAT^ATC
FauI 19 CCCGC CCCGC(4/6)
HapII 434 CCGG C^CGG
HgaI 196 GACGC GACGC(5/10)
HhaI 375 GCGC GCG^C
Hin1I 196 GACGCC G[GA]^CG[CT]C
HinP1I 375 GCGC G^CGC
HpaII 434 CCGG C^CGG
KpnI 55 GGTACC GGTAC^C
MaeII 677 ACGT A^CGT
MslI 202 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 434 CCGG C^CGG
NspI 347 ACATGT [GA]CATG^[CT]
PaeR7I 641 CTCGAG C^TCGAG
PflMI 270 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 232 GCATC GCATC(5/9)
SspBI 446 TGTACA T^GTACA
Van91I 270 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 641 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 20 CCGC CCGC(-3/-1)
358 GCGG
AfaI 56 GTAC GT^AC
447 GTAC
AlwNI 230 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
478 CAGAAACTG
AspHI 603 GTGCTC G[AT]GC[AT]^C
673 GTGCAC
BmyI 603 GTGCTC G[AGT]GC[ACT]^C
673 GTGCAC
BsaJI 275 CCGTGG C^C[ACGT][ACGT]GG
305 CCCTGG
BsiHKAI 603 GTGCTC G[AT]GC[AT]^C
673 GTGCAC
BsiYI 204 CCTGGATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCAGGCCGTGG
BslI 204 CCTGGATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
270 CCAGGCCGTGG
BsmFI 16 GTCCC GGGAC(10/14)
303 GTCCC
BsmI 361 GCATTC GAATGC(1/-1)
495 GCATTC
Bsp1286I 603 GTGCTC G[AGT]GC[ACT]^C
673 GTGCAC
Cac8I 155 GCCAGC GC[ACGT]^[ACGT]GC
293 GCTGGC
Cfr13I 15 GGTCC G^G[ACGT]CC
342 GGGCC
DdeI 459 CTCAG C^T[ACGT]AG
659 CTGAG
EarI 145 GAAGAG CTCTTC(1/4)
190 GAAGAG
HphI 690 GGTGA GGTGA(8/7)
29 TCACC
Ksp632I 145 GAAGAG CTCTTC(1/4)
190 GAAGAG
MseI 116 TTAA T^TAA
712 TTAA
MspA1I 334 CAGCTG C[AC]G^C[GT]G
368 CAGCTG
NlaIII 348 CATG CATG^
378 CATG
NlaIV 15 GGTCCC GG[ACGT]^[ACGT]CC
55 GGTACC
PvuII 334 CAGCTG CAG^CTG
368 CAGCTG
RsaI 56 GTAC GT^AC
447 GTAC
Sau96I 15 GGTCC G^G[ACGT]CC
342 GGGCC
SmlI 517 CTTGAG C^T[CT][GA]AG
641 CTCGAG
Tru9I 116 TTAA T^TAA
712 TTAA
Tsp45I 28 GTCAC ^GT[GC]AC
533 GTCAC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AlwI BbvI BssKI BstF5I
BstNI DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MaeIII MboI MboII
MnlI MvaI MwoI NdeII PstI
Sau3AI ScrFI SfcI TaqI TseI
TspRI TthHB8I