Restriction map for
pFN21AB5473
(Linear) (Six-base) MAPSORT of: KIBB5473.seq Check: 1748 from: 1 to: 783
KIBB5473.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
Acc65I G'GTAC_C
Cuts at: 0 55 783
Size: 55 728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 738 783
Size: 209 529 45
Fragments arranged by size:
529 209 45
AflIII A'CryG_T
Cuts at: 0 347 783
Size: 347 436
AlwNI CAG_nnn'CTG
Cuts at: 0 235 483 783
Size: 235 248 300
Fragments arranged by size:
300 248 235
ApaLI G'TGCA_C
Cuts at: 0 673 783
Size: 673 110
AvaI C'yCGr_G
Cuts at: 0 641 783
Size: 641 142
BanI G'GyrC_C
Cuts at: 0 55 783
Size: 55 728
BbsI GAAGACnn'nnnn_
Cuts at: 0 733 783
Size: 733 50
BbvCI CC'TCA_GC
Cuts at: 0 659 783
Size: 659 124
Bme1580I G_kGCm'C
Cuts at: 0 677 783
Size: 677 106
Bpu10I CC'TnA_GC
Cuts at: 0 659 783
Size: 659 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 783
Size: 538 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 687 783
Size: 687 96
BsaAI yAC'GTr
Cuts at: 0 678 783
Size: 678 105
BsaHI Gr'CG_yC
Cuts at: 0 197 783
Size: 197 586
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 783
Size: 163 620
BseYI C'CCAG_C
Cuts at: 0 370 783
Size: 370 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 783
Size: 308 475
BsiHKAI G_wGCw'C
Cuts at: 0 607 677 783
Size: 607 70 106
Fragments arranged by size:
607 106 70
BsmI GAATG_Cn'
Cuts at: 0 361 495 783
Size: 361 134 288
Fragments arranged by size:
361 288 134
Bsp1286I G_dGCh'C
Cuts at: 0 607 677 783
Size: 607 70 106
Fragments arranged by size:
607 106 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 783
Size: 776 7
BsrGI T'GTAC_A
Cuts at: 0 446 783
Size: 446 337
BstAPI GCAn_nnn'nTGC
Cuts at: 0 602 783
Size: 602 181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 783
Size: 208 575
Bsu36I CC'TnA_GG
Cuts at: 0 459 783
Size: 459 324
BtgI C'CryG_G
Cuts at: 0 275 783
Size: 275 508
BtsI GCAGTG_nn'
Cuts at: 0 320 783
Size: 320 463
EarI CTCTTCn'nnn_
Cuts at: 0 140 185 783
Size: 140 45 598
Fragments arranged by size:
598 140 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 738 783
Size: 209 529 45
Fragments arranged by size:
529 209 45
EcoRV GAT'ATC
Cuts at: 0 48 783
Size: 48 735
KpnI G_GTAC'C
Cuts at: 0 59 783
Size: 59 724
MslI CAynn'nnrTG
Cuts at: 0 206 783
Size: 206 577
MspA1I CmG'CkG
Cuts at: 0 336 370 783
Size: 336 34 413
Fragments arranged by size:
413 336 34
NspI r_CATG'y
Cuts at: 0 351 783
Size: 351 432
PciI A'CATG_T
Cuts at: 0 347 783
Size: 347 436
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 783
Size: 276 507
PstI C_TGCA'G
Cuts at: 0 131 231 341 783
Size: 131 100 110 442
Fragments arranged by size:
442 131 110 100
PvuII CAG'CTG
Cuts at: 0 336 370 783
Size: 336 34 413
Fragments arranged by size:
413 336 34
SfcI C'TryA_G
Cuts at: 0 127 227 337 735 783
Size: 127 100 110 398 48
Fragments arranged by size:
398 127 110 100 48
SmlI C'TyrA_G
Cuts at: 0 517 641 783
Size: 517 124 142
Fragments arranged by size:
517 142 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 590 783
Size: 3 587 193
Fragments arranged by size:
587 193 3
TatI w'GTAC_w
Cuts at: 0 446 783
Size: 446 337
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 428 460 783
Size: 428 32 323
Fragments arranged by size:
428 323 32
XhoI C'TCGA_G
Cuts at: 0 641 783
Size: 641 142
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaLI AvaI BanI BbsI
BbvCI Bme1580I Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstAPI
BstXI Bsu36I BtgI BtsI EarI Eco57MI EcoRV KpnI
MslI MspA1I NspI PciI PflMI PstI PvuII SfcI
SmlI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI XmaI XmnI ZraI