Restriction map for
pFN21AB5467
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BbeI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspHI BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 565 AACGTT AA^CGTT
AcsI 507 GAATTT [GA]^AATT[CT]
AflIII 315 ACATGT A^C[GA][CT]GT
AhdI 341 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 80 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 770 GGGCCC GGGCC^C
ApoI 507 GAATTT [GA]^AATT[CT]
AspEI 341 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 542 TGGCCA TGG^CCA
BamHI 755 GGATCC G^GATCC
BanI 917 GGCACC G^G[CT][GA]CC
BglII 836 AGATCT A^GATCT
BlpI 264 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 264 GCTCAGC GC^T[ACGT]AGC
BseRI 275 GAGGAG GAGGAG(10/8)
BsiEI 309 CGGCCG CG[GA][CT]^CG
BsmI 165 GAATGC GAATGC(1/-1)
Bsp1407I 299 TGTACA T^GTACA
BspLU11I 315 ACATGT A^CATGT
BsrGI 299 TGTACA T^GTACA
BstXI 533 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 264 GCTCAGC GC^T[ACGT]AGC
EagI 309 CGGCCG C^GGCCG
Eam1105I 341 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 225 GAAGAG CTCTTC(1/4)
EclXI 309 CGGCCG C^GGCCG
Eco52I 309 CGGCCG C^GGCCG
HincII 562 GTCAAC GT[CT]^[GA]AC
HindII 562 GTCAAC GT[CT]^[GA]AC
Ksp632I 225 GAAGAG CTCTTC(1/4)
KspI 525 CCGCGG CCGC^GG
MaeII 566 ACGT A^CGT
MluNI 542 TGGCCA TGG^CCA
MscI 542 TGGCCA TGG^CCA
MslI 958 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 975 CCATGG C^CATGG
NruI 417 TCGCGA TCG^CGA
PaeR7I 29 CTCGAG C^TCGAG
Psp1406I 565 AACGTT AA^CGTT
SacII 525 CCGCGG CCGC^GG
SfiI 529 GGCCCCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 29 CTCGAG C^T[CT][GA]AG
SspBI 299 TGTACA T^GTACA
TaqI 30 TCGA T^CGA
Tsp45I 748 GTCAC ^GT[GC]AC
Tsp509I 508 AATT ^AATT
TthHB8I 30 TCGA T^CGA
XhoI 29 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 756 GATCC GGATC(4/5)
755 GGATC
Aor51HI 33 AGCGCT AGC^GCT
514 AGCGCT
AspHI 413 GAGCTC G[AT]GC[AT]^C
673 GAGCTC
BbvCI 849 CCTCAGC CCTCAGC(-5/-2)
466 GCTGAGG
BglI 530 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
883 GCCCACAGGGC
BsiHKAI 413 GAGCTC G[AT]GC[AT]^C
673 GAGCTC
BstF5I 213 GGATG GGATG(2/0)
963 GGATG
BstYI 755 GGATCC [GA]^GATC[CT]
836 AGATCT
EaeI 309 CGGCCG [CT]^GGCC[GA]
542 TGGCCA
Eco47III 33 AGCGCT AGC^GCT
514 AGCGCT
EcoT14I 878 CCTTGG C^C[AT][AT]GG
975 CCATGG
FauI 524 CCCGC CCCGC(4/6)
729 CCCGC
FokI 213 GGATG GGATG(9/13)
963 GGATG
MaeIII 748 GTCAC ^GT[ACGT]AC
857 GTAAC
MflI 755 GGATCC [GA]^GATC[CT]
836 AGATCT
MseI 259 TTAA T^TAA
605 TTAA
MspA1I 525 CCGCGG C[AC]G^C[GT]G
852 CAGCGG
NaeI 427 GCCGGC GCC^GGC
863 GCCGGC
NgoMIV 427 GCCGGC G^CCGGC
863 GCCGGC
NspI 315 ACATGT [GA]CATG^[CT]
957 GCATGT
PpuMI 363 AGGTCCC [GA]G^G[AT]CC[CT]
908 GGGACCC
PstI 8 CTGCAG CTGCA^G
871 CTGCAG
SacI 413 GAGCTC GAGCT^C
673 GAGCTC
SfcI 8 CTGCAG C^T[GA][CT]AG
871 CTGCAG
StyI 878 CCTTGG C^C[AT][AT]GG
975 CCATGG
TfiI 688 GAATC G^A[AT]TC
822 GAATC
Tru9I 259 TTAA T^TAA
605 TTAA
XhoII 755 GGATCC [GA]^GATC[CT]
836 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TseI TspRI