Restriction map for
pFN21AB5467
(Linear) (Six-base) MAPSORT of: KIBB5467.seq Check: 7155 from: 1 to: 984
KIBB5467.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AclI AA'CG_TT
Cuts at: 0 566 984
Size: 566 418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 775 984
Size: 244 531 209
Fragments arranged by size:
531 244 209
AfeI AGC'GCT
Cuts at: 0 35 516 984
Size: 35 481 468
Fragments arranged by size:
481 468 35
AflIII A'CryG_T
Cuts at: 0 315 984
Size: 315 669
AhdI GACnn_n'nnGTC
Cuts at: 0 346 984
Size: 346 638
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 984
Size: 664 32 288
Fragments arranged by size:
664 288 32
AlwNI CAG_nnn'CTG
Cuts at: 0 85 984
Size: 85 899
ApaI G_GGCC'C
Cuts at: 0 774 984
Size: 774 210
ApoI r'AATT_y
Cuts at: 0 507 984
Size: 507 477
AvaI C'yCGr_G
Cuts at: 0 29 91 922 984
Size: 29 62 831 62
Fragments arranged by size:
831 62 62 29
BamHI G'GATC_C
Cuts at: 0 755 984
Size: 755 229
BanI G'GyrC_C
Cuts at: 0 917 984
Size: 917 67
BanII G_rGCy'C
Cuts at: 0 208 417 677 718 774 984
Size: 208 209 260 41 56 210
Fragments arranged by size:
260 210 209 208 56 41
BbvCI CC'TCA_GC
Cuts at: 0 467 850 984
Size: 467 383 134
Fragments arranged by size:
467 383 134
BglI GCCn_nnn'nGGC
Cuts at: 0 536 889 984
Size: 536 353 95
Fragments arranged by size:
536 353 95
BglII A'GATC_T
Cuts at: 0 836 984
Size: 836 148
BlpI GC'TnA_GC
Cuts at: 0 265 984
Size: 265 719
Bme1580I G_kGCm'C
Cuts at: 0 774 894 984
Size: 774 120 90
Fragments arranged by size:
774 120 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 64 984
Size: 64 920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 44 301 385 984
Size: 44 257 84 599
Fragments arranged by size:
599 257 84 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 293 380 691 984
Size: 106 187 87 311 293
Fragments arranged by size:
311 293 187 106 87
Bpu10I CC'TnA_GC
Cuts at: 0 467 664 814 850 984
Size: 467 197 150 36 134
Fragments arranged by size:
467 197 150 134 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 168 198 664 694 984
Size: 168 30 466 30 290
Fragments arranged by size:
466 290 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 290 984
Size: 290 694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 317 493 984
Size: 35 282 176 491
Fragments arranged by size:
491 282 176 35
BsiEI CG_ry'CG
Cuts at: 0 312 984
Size: 312 672
BsiHKAI G_wGCw'C
Cuts at: 0 417 677 984
Size: 417 260 307
Fragments arranged by size:
417 307 260
BsmI GAATG_Cn'
Cuts at: 0 171 984
Size: 171 813
Bsp1286I G_dGCh'C
Cuts at: 0 208 417 677 718 774 894 984
Size: 208 209 260 41 56 120 90
Fragments arranged by size:
260 209 208 120 90 56 41
BsrFI r'CCGG_y
Cuts at: 0 307 427 863 984
Size: 307 120 436 121
Fragments arranged by size:
436 307 121 120
BsrGI T'GTAC_A
Cuts at: 0 299 984
Size: 299 685
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 984
Size: 540 444
BstYI r'GATC_y
Cuts at: 0 755 836 984
Size: 755 81 148
Fragments arranged by size:
755 148 81
BtgI C'CryG_G
Cuts at: 0 209 525 975 984
Size: 209 316 450 9
Fragments arranged by size:
450 316 209 9
EaeI y'GGCC_r
Cuts at: 0 309 542 984
Size: 309 233 442
Fragments arranged by size:
442 309 233
EagI C'GGCC_G
Cuts at: 0 309 984
Size: 309 675
EarI CTCTTCn'nnn_
Cuts at: 0 220 984
Size: 220 764
EcoICRI GAG'CTC
Cuts at: 0 415 675 984
Size: 415 260 309
Fragments arranged by size:
415 309 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 244 293 380 691 775 984
Size: 106 138 49 87 311 84 209
Fragments arranged by size:
311 209 138 106 87 84 49
EcoO109I rG'GnC_Cy
Cuts at: 0 142 156 364 771 909 940 984
Size: 142 14 208 407 138 31 44
Fragments arranged by size:
407 208 142 138 44 31 14
HaeII r_GCGC'y
Cuts at: 0 26 37 518 933 984
Size: 26 11 481 415 51
Fragments arranged by size:
481 415 51 26 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 807 839 984
Size: 332 32 443 32 145
Fragments arranged by size:
443 332 145 32 32
HincII GTy'rAC
Cuts at: 0 564 984
Size: 564 420
MscI TGG'CCA
Cuts at: 0 544 984
Size: 544 440
MslI CAynn'nnrTG
Cuts at: 0 962 984
Size: 962 22
MspA1I CmG'CkG
Cuts at: 0 527 854 984
Size: 527 327 130
Fragments arranged by size:
527 327 130
NaeI GCC'GGC
Cuts at: 0 429 865 984
Size: 429 436 119
Fragments arranged by size:
436 429 119
NcoI C'CATG_G
Cuts at: 0 975 984
Size: 975 9
NgoMIV G'CCGG_C
Cuts at: 0 427 863 984
Size: 427 436 121
Fragments arranged by size:
436 427 121
NruI TCG'CGA
Cuts at: 0 419 984
Size: 419 565
NspI r_CATG'y
Cuts at: 0 319 961 984
Size: 319 642 23
Fragments arranged by size:
642 319 23
PciI A'CATG_T
Cuts at: 0 315 984
Size: 315 669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 984
Size: 664 32 288
Fragments arranged by size:
664 288 32
PpuMI rG'GwC_Cy
Cuts at: 0 364 909 984
Size: 364 545 75
Fragments arranged by size:
545 364 75
PspOMI G'GGCC_C
Cuts at: 0 770 984
Size: 770 214
PspXI vC'TCGA_Gb
Cuts at: 0 29 984
Size: 29 955
PstI C_TGCA'G
Cuts at: 0 12 875 984
Size: 12 863 109
Fragments arranged by size:
863 109 12
SacI G_AGCT'C
Cuts at: 0 417 677 984
Size: 417 260 307
Fragments arranged by size:
417 307 260
SacII CC_GC'GG
Cuts at: 0 528 984
Size: 528 456
SanDI GG'GwC_CC
Cuts at: 0 909 984
Size: 909 75
SfcI C'TryA_G
Cuts at: 0 8 871 984
Size: 8 863 113
Fragments arranged by size:
863 113 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 536 984
Size: 536 448
SmlI C'TyrA_G
Cuts at: 0 29 984
Size: 29 955
StyI C'CwwG_G
Cuts at: 0 878 975 984
Size: 878 97 9
Fragments arranged by size:
878 97 9
TatI w'GTAC_w
Cuts at: 0 299 984
Size: 299 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 168 200 984
Size: 119 32 17 32 784
Fragments arranged by size:
784 119 32 32 17
XhoI C'TCGA_G
Cuts at: 0 29 984
Size: 29 955
Enzymes that do cut:
AclI AcuI AfeI AflIII AhdI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbvCI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaXI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrFI BsrGI BstXI
BstYI BtgI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NruI NspI PciI PpiI PpuMI PspOMI PspXI
PstI SacI SacII SanDI SfcI SfiI SmlI StyI
TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BcgI BciVI BclI BfrBI BmgBI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NsiI PacI PasI
PflMI PfoI PmeI PmlI PshAI PsiI PsrI PvuI
PvuII RsrII SalI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XmaI XmnI
ZraI