(Circular) (Six-base) MAPSORT of: pFN21AB5412.seq Check: 6545 from: 1 to: 5761 pFN21AB5412 5761 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3824 5208 5476 1632 Size: 2192 1384 268 1917 Fragments arranged by size: 2192 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 5729 AlwNI CAG_nnn'CTG Cuts at: 2234 3002 3504 5076 2234 Size: 768 502 1572 2919 Fragments arranged by size: 2919 1572 768 502 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2333 2421 2449 2799 3070 3296 3448 5557 Size: 840 88 28 350 271 226 152 2109 Cuts at: 5557 1493 Size: 1697 Fragments arranged by size: 2109 1697 840 350 271 226 152 88 28 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2084 3086 1870 Size: 73 141 1002 4545 Fragments arranged by size: 4545 1002 141 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3082 4537 618 Size: 325 217 627 1295 1455 1842 Fragments arranged by size: 1842 1455 1295 627 325 217 BanII G_rGCy'C Cuts at: 1391 2154 3080 1391 Size: 763 926 4072 Fragments arranged by size: 4072 926 763 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2497 1548 Size: 949 4812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2869 5756 2869 Size: 2887 2874 BlpI GC'TnA_GC Cuts at: 2546 3178 2546 Size: 632 5129 Bme1580I G_kGCm'C Cuts at: 1790 2379 3822 4978 1790 Size: 589 1443 1156 2573 Fragments arranged by size: 2573 1443 1156 589 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3031 3492 4450 Size: 416 53 83 399 24 1614 461 958 Cuts at: 4450 442 Size: 1753 Fragments arranged by size: 1753 1614 958 461 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 2620 4421 1239 Size: 1274 107 1801 2579 Fragments arranged by size: 2579 1801 1274 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2038 2766 3886 4751 5049 2038 Size: 728 1120 865 298 2750 Fragments arranged by size: 2750 1120 865 728 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2136 4424 882 Size: 617 637 2288 2219 Fragments arranged by size: 2288 2219 637 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 1178 Size: 30 165 30 1203 30 4303 Fragments arranged by size: 4303 1203 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2138 2144 2702 2930 1186 Size: 949 3 6 558 228 4017 Fragments arranged by size: 4017 949 558 228 6 3 BseYI C'CCAG_C Cuts at: 2643 4964 5460 2643 Size: 2321 496 2944 Fragments arranged by size: 2944 2321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 3822 3907 4978 2154 Size: 1668 85 1071 2937 Fragments arranged by size: 2937 1668 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 BsmBI CGTCTCn'nnnn_ Cuts at: 2093 2626 2093 Size: 533 5228 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2379 3080 3822 3907 4978 1391 Size: 399 364 225 701 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 701 399 364 225 85 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2704 4256 4430 65 Size: 1183 1456 1552 174 1396 Fragments arranged by size: 1552 1456 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 4405 4592 1700 Size: 110 234 2361 187 2869 Fragments arranged by size: 2869 2361 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 2204 2716 2896 794 Size: 1410 512 180 3659 Fragments arranged by size: 3659 1410 512 180 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2869 3091 3458 3843 3860 5312 5398 5410 Size: 897 222 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 897 385 367 346 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2381 2511 513 Size: 552 1316 130 3763 Fragments arranged by size: 3763 1316 552 130 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726 Size: 54 1425 50 275 18 1378 889 1629 Cuts at: 5726 8 Size: 43 Fragments arranged by size: 1629 1425 1378 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2687 2928 3691 1353 Size: 984 350 241 763 3423 Fragments arranged by size: 3423 984 763 350 241 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 2152 Size: 5761 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2620 3824 4421 5208 5476 1239 Size: 393 881 107 1204 597 787 268 1524 Fragments arranged by size: 1524 1204 881 787 597 393 268 107 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2861 2159 Size: 702 5059 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 4908 1678 Size: 3230 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2550 2582 4402 Size: 32 135 32 506 32 440 32 1820 Cuts at: 4402 4434 4476 4508 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1820 506 440 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2389 3105 3351 669 Size: 988 281 451 716 246 3079 Fragments arranged by size: 3079 988 716 451 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3939 Fragments arranged by size: 3939 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3719 4078 4237 518 Size: 648 2553 359 159 2042 Fragments arranged by size: 2553 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2372 2438 2722 3177 3854 5002 5247 1606 Size: 766 66 284 455 677 1148 245 2120 Fragments arranged by size: 2120 1148 766 677 455 284 245 66 NcoI C'CATG_G Cuts at: 513 1065 2381 513 Size: 552 1316 3893 Fragments arranged by size: 3893 1316 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PfoI T'CCnGG_A Cuts at: 2529 2529 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2597 2629 2597 Size: 32 5729 PstI C_TGCA'G Cuts at: 830 2133 2947 2967 3113 830 Size: 1303 814 20 146 3478 Fragments arranged by size: 3478 1303 814 146 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2372 2438 2372 Size: 66 5695 SacI G_AGCT'C Cuts at: 2154 2154 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 2337 2687 2337 Size: 350 5411 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2129 2943 2963 3109 4244 4925 5116 Size: 219 1084 814 20 146 1135 681 191 Cuts at: 5116 826 Size: 1471 Fragments arranged by size: 1471 1135 1084 814 681 219 191 146 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2017 2781 3865 4766 5028 820 Size: 197 926 74 764 1084 901 262 1553 Fragments arranged by size: 1553 1084 926 901 764 262 197 74 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 2893 152 Size: 2741 3020 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2381 3200 513 Size: 552 1316 819 3074 Fragments arranged by size: 3074 1316 819 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 3805 3958 4143 4588 325 Size: 1255 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4007 5719 96 Size: 274 80 33 51 1443 2030 1712 138 Fragments arranged by size: 2030 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 2409 3890 2409 Size: 1481 4280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI