(Circular) (Six-base) MAPSORT of: pFN21AB5360.seq Check: 4256 from: 1 to: 5809 pFN21AB5360 5809 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 1937 Size: 1215 4594 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2790 3872 5256 5524 1632 Size: 1158 1082 1384 268 1917 Fragments arranged by size: 1917 1384 1158 1082 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 2901 3552 5124 2901 Size: 651 1572 3586 Fragments arranged by size: 3586 1572 651 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 2612 3032 3118 3344 3496 5605 1493 Size: 1119 420 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 1119 420 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 3134 1870 Size: 73 1191 4545 Fragments arranged by size: 4545 1191 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2861 3130 4585 618 Size: 325 217 627 1074 269 1455 1842 Fragments arranged by size: 1842 1455 1074 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 3128 1391 Size: 1737 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BbvCI CC'TCA_GC Cuts at: 2348 2348 Size: 5809 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2467 3701 4911 2467 Size: 1234 1210 3365 Fragments arranged by size: 3365 1234 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4420 136 Size: 107 122 71 631 605 2748 1525 Fragments arranged by size: 2748 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5804 5804 Size: 5809 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 3870 5026 1790 Size: 2080 1156 2573 Fragments arranged by size: 2573 2080 1156 BmgBI CAC'GTC Cuts at: 1318 2536 1318 Size: 1218 4591 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 2451 3540 Size: 416 53 83 399 24 854 180 1089 Cuts at: 3540 4498 442 Size: 958 1753 Fragments arranged by size: 1753 1089 958 854 416 399 180 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 4469 1239 Size: 1066 2164 2579 Fragments arranged by size: 2579 2164 1066 Bpu10I CC'TnA_GC Cuts at: 2348 2348 Size: 5809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3934 4799 5097 3934 Size: 865 298 4646 Fragments arranged by size: 4646 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4472 882 Size: 617 2973 2219 Fragments arranged by size: 2973 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 2369 5803 2369 Size: 3434 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3616 3998 4633 275 Size: 53 83 186 936 2083 382 635 1451 Fragments arranged by size: 2083 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4242 4640 4914 5061 5472 4242 Size: 398 274 147 411 4579 Fragments arranged by size: 4579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2098 2128 2794 2824 1178 Size: 30 165 30 695 30 666 30 4163 Fragments arranged by size: 4163 695 666 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2802 1186 Size: 1616 4193 BseYI C'CCAG_C Cuts at: 2208 2247 2448 5012 5508 2208 Size: 39 201 2564 496 2509 Fragments arranged by size: 2564 2509 496 201 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2483 3259 4020 5048 1540 Size: 275 186 482 776 761 1028 2301 Fragments arranged by size: 2301 1028 776 761 482 275 186 BsiHKAI G_wGCw'C Cuts at: 3870 3955 5026 3870 Size: 85 1071 4653 Fragments arranged by size: 4653 1071 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3128 3870 3955 5026 1391 Size: 399 1338 742 85 1071 2174 Fragments arranged by size: 2174 1338 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3164 844 Size: 2320 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3696 1394 Size: 429 1873 3507 Fragments arranged by size: 3507 1873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4478 65 Size: 1183 3056 174 1396 Fragments arranged by size: 3056 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4453 4640 1700 Size: 110 2643 187 2869 Fragments arranged by size: 2869 2643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 3869 4881 3869 Size: 1012 4797 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstXI CCAn_nnnn'nTGG Cuts at: 2554 2554 Size: 5809 BstYI r'GATC_y Cuts at: 1972 2879 3139 3506 3891 3908 5360 5446 5458 Size: 907 260 367 385 17 1452 86 12 Cuts at: 5458 5804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 385 367 346 260 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2026 2814 2026 Size: 788 5021 BtgI C'CryG_G Cuts at: 513 1065 2547 513 Size: 552 1482 3775 Fragments arranged by size: 3775 1482 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2068 3325 4119 4139 2068 Size: 1257 794 20 3738 Fragments arranged by size: 3738 1257 794 20 ClaI AT'CG_AT Cuts at: 1554 2785 1554 Size: 1231 4578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2139 2174 529 Size: 35 1575 35 4164 Fragments arranged by size: 4164 1575 35 35 DraI TTT'AAA Cuts at: 2463 2668 2776 3113 2463 Size: 205 108 337 5159 Fragments arranged by size: 5159 337 205 108 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774 Size: 54 1425 50 275 18 1426 889 1629 Cuts at: 5774 8 Size: 43 Fragments arranged by size: 1629 1426 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3078 3739 1353 Size: 1725 661 3423 Fragments arranged by size: 3423 1725 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4396 4770 4916 387 Size: 1064 138 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2790 3872 4469 5256 5524 1239 Size: 393 673 485 1082 597 787 268 1524 Fragments arranged by size: 1524 1082 787 673 597 485 393 268 EcoO109I rG'GnC_Cy Cuts at: 2344 2575 2344 Size: 231 5578 EcoRI G'AATT_C Cuts at: 2612 3118 3496 2612 Size: 506 378 4925 Fragments arranged by size: 4925 506 378 FspI TGC'GCA Cuts at: 4315 4315 Size: 5809 HaeII r_GCGC'y Cuts at: 1678 4956 1678 Size: 3278 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2401 2433 2465 2497 2572 Size: 32 135 32 829 32 32 32 75 Cuts at: 2572 2604 2614 2646 4450 4482 4524 4556 1373 Size: 32 10 32 1804 32 42 32 2626 Fragments arranged by size: 2626 1804 829 135 75 42 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4923 5107 1305 Size: 3618 184 2007 Fragments arranged by size: 3618 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3987 Fragments arranged by size: 3987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 2652 3767 4126 4285 518 Size: 648 1386 100 1115 359 159 2042 Fragments arranged by size: 2042 1386 1115 648 359 159 100 MspA1I CmG'CkG Cuts at: 1606 2430 3225 3902 5050 5295 1606 Size: 824 795 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 795 677 245 NcoI C'CATG_G Cuts at: 513 1065 2547 513 Size: 552 1482 3775 Fragments arranged by size: 3775 1482 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 2056 3167 4712 1132 Size: 97 827 1111 1545 2229 Fragments arranged by size: 2229 1545 1111 827 97 PasI CC'CwG_GG Cuts at: 1348 2701 1348 Size: 1353 4456 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 2340 2406 1108 Size: 1232 66 4511 Fragments arranged by size: 4511 1232 66 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2344 2344 Size: 5809 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3039 3379 3039 Size: 340 5469 PstI C_TGCA'G Cuts at: 830 2569 3161 830 Size: 1739 592 3478 Fragments arranged by size: 3478 1739 592 PvuI CG_AT'CG Cuts at: 2001 2483 2001 Size: 482 5327 PvuII CAG'CTG Cuts at: 2430 2430 Size: 5809 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SapI GCTCTTCn'nnn_ Cuts at: 3078 3078 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2070 2425 2565 3157 4292 4973 5164 Size: 219 1025 355 140 592 1135 681 191 Cuts at: 5164 826 Size: 1471 Fragments arranged by size: 1471 1135 1025 681 592 355 219 191 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3913 4814 5076 820 Size: 197 926 1970 901 262 1553 Fragments arranged by size: 1970 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5809 StyI C'CwwG_G Cuts at: 513 1065 2547 3248 513 Size: 552 1482 701 3074 Fragments arranged by size: 3074 1482 701 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 3853 4006 4191 4636 325 Size: 1255 2256 17 153 185 445 1498 Fragments arranged by size: 2256 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4055 5767 96 Size: 274 80 33 51 1443 2078 1712 138 Fragments arranged by size: 2078 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2794 2826 5769 5801 2794 Size: 32 2943 32 2802 Fragments arranged by size: 2943 2802 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 2855 2554 Size: 301 5508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI