Restriction map for
pFN21AB8735
(Circular) (Six-base) MAPSORT of: pFN21AB8735.seq Check: 8328 from: 1 to: 5527
pFN21AB8735 5527 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2658 2658
Size: 5527
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 4974 5242 1632
Size: 1958 1384 268 1917
Fragments arranged by size:
1958 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 2028 2442 4426 5403 1203
Size: 825 414 1984 977 1327
Fragments arranged by size:
1984 1327 977 825 414
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2120 1184
Size: 936 4591
AlwNI CAG_nnn'CTG
Cuts at: 2155 3270 4842 2155
Size: 1115 1572 2840
Fragments arranged by size:
2840 1572 1115
ApaI G_GGCC'C
Cuts at: 2322 2322
Size: 5527
ApaLI G'TGCA_C
Cuts at: 2291 2297 3584 4740 2291
Size: 6 1287 1156 3078
Fragments arranged by size:
3078 1287 1156 6
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2212 2852 1870
Size: 73 243 26 640 4545
Fragments arranged by size:
4545 640 243 73 26
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2124 2221 2260 2398
Size: 325 217 627 234 103 97 39 138
Cuts at: 2398 2848 4303 618
Size: 450 1455 1842
Fragments arranged by size:
1842 1455 627 450 325 234 217 138
103 97 39
BanII G_rGCy'C
Cuts at: 1391 2178 2210 2322 2748 2763 2846 1391
Size: 787 32 112 426 15 83 4072
Fragments arranged by size:
4072 787 426 112 83 32 15
BbeI G_GCGC'C
Cuts at: 2128 2225 2264 2128
Size: 97 39 5391
Fragments arranged by size:
5391 97 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2713 928
Size: 971 814 3742
Fragments arranged by size:
3742 971 814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3718 3752 1903
Size: 34 1781 34 3678
Fragments arranged by size:
3678 1781 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2214 2407 4138
Size: 107 122 71 631 605 542 193 1731
Cuts at: 4138 136
Size: 1525
Fragments arranged by size:
1731 1525 631 605 542 193 122 107
71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2747 2944 2747
Size: 197 5330
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2295 2301 2322 3588 4744 1790
Size: 234 271 6 21 1266 1156 2573
Fragments arranged by size:
2573 1266 1156 271 234 21 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2276 2434 2786 4187 1239
Size: 953 84 158 352 1401 2579
Fragments arranged by size:
2579 1401 953 352 158 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 2797 4190 882
Size: 617 576 722 1393 2219
Fragments arranged by size:
2219 1393 722 617 576
BsaAI yAC'GTr
Cuts at: 493 1204 2040 2290 2296 3318 493
Size: 711 836 250 6 1022 2702
Fragments arranged by size:
2702 1022 836 711 250 6
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2125 2222 2261 2782
Size: 53 83 186 936 592 97 39 521
Cuts at: 2782 3334 3716 4351 275
Size: 552 382 635 1451
Fragments arranged by size:
1451 936 635 592 552 521 382 186
97 83 53 39
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 4566
BseYI C'CCAG_C
Cuts at: 2600 2654 2683 2726 4730 5226 2600
Size: 54 29 43 2004 496 2901
Fragments arranged by size:
2901 2004 496 54 43 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 2630 1163
Size: 573 558 336 4060
Fragments arranged by size:
4060 573 558 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2707 2977 3738 4766 1540
Size: 275 186 706 270 761 1028 2301
Fragments arranged by size:
2301 1028 761 706 275 270 186
BsiHKAI G_wGCw'C
Cuts at: 2295 2301 2514 2748 2772 3588 3673 4744 2295
Size: 6 213 234 24 816 85 1071 3078
Fragments arranged by size:
3078 1071 816 234 213 85 24 6
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2178 2210 2295 2301 2322 2514
Size: 399 234 154 32 85 6 21 192
Cuts at: 2514 2748 2763 2772 2846 3588 3673 4744 1391
Size: 234 15 9 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 234 234 192 154
85 85 74 32 21 15 9 6
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2658 2882 844
Size: 1319 495 224 3489
Fragments arranged by size:
3489 1319 495 224
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 3414 1394
Size: 429 791 800 3507
Fragments arranged by size:
3507 800 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4022 4196 65
Size: 1183 2774 174 1396
Fragments arranged by size:
2774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2263 2788 4171 4358 1700
Size: 110 238 215 525 1383 187 2869
Fragments arranged by size:
2869 1383 525 238 215 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 2158 1925
Size: 233 5294
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2305 794
Size: 1361 150 4016
Fragments arranged by size:
4016 1361 150
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstXI CCAn_nnnn'nTGG
Cuts at: 2412 2412
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522
Size: 885 367 385 17 1452 86 12 346
Cuts at: 5522 1972
Size: 1977
Fragments arranged by size:
1977 1452 885 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2314 2405 2707 513
Size: 552 939 310 91 302 3333
Fragments arranged by size:
3333 939 552 310 302 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3837 3857 3043
Size: 794 20 4713
Fragments arranged by size:
4713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2710 2974
Size: 54 1425 50 275 18 369 511 264
Cuts at: 2974 3863 5492 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 511 369 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 2621 3457 1353
Size: 1107 161 836 3423
Fragments arranged by size:
3423 1107 836 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2746 2746
Size: 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2276 2434 2786 3590 4187 4974
Size: 393 560 84 158 352 804 597 787
Cuts at: 4974 5242 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 597 560 393 352 268
158 84
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 5527
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2128 2225 2264 2645 2661 4674 1678
Size: 450 97 39 381 16 2013 2531
Fragments arranged by size:
2531 2013 450 381 97 39 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4168 4200 4242 4274 1373
Size: 32 135 32 2596 32 42 32 2626
Fragments arranged by size:
2626 2596 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KasI G'GCGC_C
Cuts at: 2124 2221 2260 2124
Size: 97 39 5391
Fragments arranged by size:
5391 97 39
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3485 3844 4003 518
Size: 648 843 1476 359 159 2042
Fragments arranged by size:
2042 1476 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2730 2943 3620 4768 5013 1606
Size: 699 425 213 677 1148 245 2120
Fragments arranged by size:
2120 1148 699 677 425 245 213
NaeI GCC'GGC
Cuts at: 2050 2265 2050
Size: 215 5312
NarI GG'CG_CC
Cuts at: 2125 2222 2261 2125
Size: 97 39 5391
Fragments arranged by size:
5391 97 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NgoMIV G'CCGG_C
Cuts at: 2048 2263 2048
Size: 215 5312
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2446 2885 4430 1132
Size: 97 1217 439 1545 2229
Fragments arranged by size:
2229 1545 1217 439 97
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 4858
PciI A'CATG_T
Cuts at: 2442 4426 2442
Size: 1984 3543
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5527
PfoI T'CCnGG_A
Cuts at: 2619 2619
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PmlI CAC'GTG
Cuts at: 2040 2290 2296 2040
Size: 250 6 5271
Fragments arranged by size:
5271 250 6
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2318 2318
Size: 5527
PspXI vC'TCGA_Gb
Cuts at: 2186 2186
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2099 2700 2879 830
Size: 1269 601 179 3478
Fragments arranged by size:
3478 1269 601 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
PvuII CAG'CTG
Cuts at: 2730 2730
Size: 5527
SacI G_AGCT'C
Cuts at: 2748 2748
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SapI GCTCTTCn'nnn_
Cuts at: 2621 2621
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2700 2879 2700
Size: 179 5348
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2095 2500 2696 2817 2875 4010 4691
Size: 219 1050 405 196 121 58 1135 681
Cuts at: 4691 4882 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1050 681 405 219 196 191
121 58
SfoI GGC'GCC
Cuts at: 2126 2223 2262 2126
Size: 97 39 5391
Fragments arranged by size:
5391 97 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2214 2854 1872
Size: 342 640 4545
Fragments arranged by size:
4545 640 342
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 3631 4532 4794 820
Size: 197 926 243 1445 901 262 1553
Fragments arranged by size:
1553 1445 926 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2004 2966 513
Size: 552 939 962 3074
Fragments arranged by size:
3074 962 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2361 2750 3554 3571 3724 3909 4354
Size: 1255 781 389 804 17 153 185 445
Cuts at: 4354 325
Size: 1498
Fragments arranged by size:
1498 1255 804 781 445 389 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3773 5485 96
Size: 274 80 33 51 1443 1796 1712 138
Fragments arranged by size:
1796 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5487 5519 5487
Size: 32 5495
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 2186 1943
Size: 243 5284
XmaI C'CCGG_G
Cuts at: 1870 2212 2852 1870
Size: 342 640 4545
Fragments arranged by size:
4545 640 342
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI NruI NsiI PacI PpuMI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI