Restriction map for
pFN21AB8735
(Linear) (Six-base) MAPSORT of: KIBB8735.seq Check: 7786 from: 1 to: 822
KIBB8735.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 653 822
Size: 653 169
AflIII A'CryG_T
Cuts at: 0 23 437 822
Size: 23 414 385
Fragments arranged by size:
414 385 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 822
Size: 115 707
AlwNI CAG_nnn'CTG
Cuts at: 0 150 822
Size: 150 672
ApaI G_GGCC'C
Cuts at: 0 317 822
Size: 317 505
ApaLI G'TGCA_C
Cuts at: 0 286 292 822
Size: 286 6 530
Fragments arranged by size:
530 286 6
AvaI C'yCGr_G
Cuts at: 0 181 207 822
Size: 181 26 615
Fragments arranged by size:
615 181 26
BanI G'GyrC_C
Cuts at: 0 16 119 216 255 393 822
Size: 16 103 97 39 138 429
Fragments arranged by size:
429 138 103 97 39 16
BanII G_rGCy'C
Cuts at: 0 173 205 317 743 758 822
Size: 173 32 112 426 15 64
Fragments arranged by size:
426 173 112 64 32 15
BbeI G_GCGC'C
Cuts at: 0 123 220 259 822
Size: 123 97 39 563
Fragments arranged by size:
563 123 97 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 822
Size: 708 114
BglI GCCn_nnn'nGGC
Cuts at: 0 209 402 822
Size: 209 193 420
Fragments arranged by size:
420 209 193
BlpI GC'TnA_GC
Cuts at: 0 742 822
Size: 742 80
Bme1580I G_kGCm'C
Cuts at: 0 19 290 296 317 822
Size: 19 271 6 21 505
Fragments arranged by size:
505 271 21 19 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 271 429 781 822
Size: 187 84 158 352 41
Fragments arranged by size:
352 187 158 84 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 792 822
Size: 70 722 30
Fragments arranged by size:
722 70 30
BsaAI yAC'GTr
Cuts at: 0 35 285 291 822
Size: 35 250 6 531
Fragments arranged by size:
531 250 35 6
BsaHI Gr'CG_yC
Cuts at: 0 120 217 256 777 822
Size: 120 97 39 521 45
Fragments arranged by size:
521 120 97 45 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 822
Size: 142 680
BseYI C'CCAG_C
Cuts at: 0 595 649 678 721 822
Size: 595 54 29 43 101
Fragments arranged by size:
595 101 54 43 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 625 822
Size: 289 336 197
Fragments arranged by size:
336 289 197
BsiEI CG_ry'CG
Cuts at: 0 702 822
Size: 702 120
BsiHKAI G_wGCw'C
Cuts at: 0 290 296 509 743 767 822
Size: 290 6 213 234 24 55
Fragments arranged by size:
290 234 213 55 24 6
Bsp1286I G_dGCh'C
Cuts at: 0 19 173 205 290 296 317 509 743
Size: 19 154 32 85 6 21 192 234
Cuts at: 743 758 767 822
Size: 15 9 55
Fragments arranged by size:
234 192 154 85 55 32 21 19
15 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 653 822
Size: 158 495 169
Fragments arranged by size:
495 169 158
BsrBI CCG'CTC
Cuts at: 0 609 822
Size: 609 213
BsrFI r'CCGG_y
Cuts at: 0 43 258 783 822
Size: 43 215 525 39
Fragments arranged by size:
525 215 43 39
BssHII G'CGCG_C
Cuts at: 0 153 822
Size: 153 669
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 300 822
Size: 150 150 522
Fragments arranged by size:
522 150 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 407 822
Size: 407 415
BtgI C'CryG_G
Cuts at: 0 309 400 702 822
Size: 309 91 302 120
Fragments arranged by size:
309 302 120 91
EaeI y'GGCC_r
Cuts at: 0 194 705 822
Size: 194 511 117
Fragments arranged by size:
511 194 117
EarI CTCTTCn'nnn_
Cuts at: 0 455 616 822
Size: 455 161 206
Fragments arranged by size:
455 206 161
EcoICRI GAG'CTC
Cuts at: 0 741 822
Size: 741 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 271 429 781 822
Size: 187 84 158 352 41
Fragments arranged by size:
352 187 158 84 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 627 822
Size: 627 195
HaeII r_GCGC'y
Cuts at: 0 123 220 259 640 656 822
Size: 123 97 39 381 16 166
Fragments arranged by size:
381 166 123 97 39 16
KasI G'GCGC_C
Cuts at: 0 119 216 255 822
Size: 119 97 39 567
Fragments arranged by size:
567 119 97 39
MspA1I CmG'CkG
Cuts at: 0 300 725 822
Size: 300 425 97
Fragments arranged by size:
425 300 97
NaeI GCC'GGC
Cuts at: 0 45 260 822
Size: 45 215 562
Fragments arranged by size:
562 215 45
NarI GG'CG_CC
Cuts at: 0 120 217 256 822
Size: 120 97 39 566
Fragments arranged by size:
566 120 97 39
NgoMIV G'CCGG_C
Cuts at: 0 43 258 822
Size: 43 215 564
Fragments arranged by size:
564 215 43
NspI r_CATG'y
Cuts at: 0 441 822
Size: 441 381
PasI CC'CwG_GG
Cuts at: 0 12 822
Size: 12 810
PciI A'CATG_T
Cuts at: 0 437 822
Size: 437 385
PfoI T'CCnGG_A
Cuts at: 0 614 822
Size: 614 208
PmlI CAC'GTG
Cuts at: 0 35 285 291 822
Size: 35 250 6 531
Fragments arranged by size:
531 250 35 6
PspOMI G'GGCC_C
Cuts at: 0 313 822
Size: 313 509
PspXI vC'TCGA_Gb
Cuts at: 0 181 822
Size: 181 641
PstI C_TGCA'G
Cuts at: 0 94 695 822
Size: 94 601 127
Fragments arranged by size:
601 127 94
PvuII CAG'CTG
Cuts at: 0 725 822
Size: 725 97
SacI G_AGCT'C
Cuts at: 0 743 822
Size: 743 79
SapI GCTCTTCn'nnn_
Cuts at: 0 616 822
Size: 616 206
SbfI CC_TGCA'GG
Cuts at: 0 695 822
Size: 695 127
SfcI C'TryA_G
Cuts at: 0 90 495 691 812 822
Size: 90 405 196 121 10
Fragments arranged by size:
405 196 121 90 10
SfoI GGC'GCC
Cuts at: 0 121 218 257 822
Size: 121 97 39 565
Fragments arranged by size:
565 121 97 39
SmaI CCC'GGG
Cuts at: 0 209 822
Size: 209 613
SmlI C'TyrA_G
Cuts at: 0 181 822
Size: 181 641
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 356 745 822
Size: 356 389 77
Fragments arranged by size:
389 356 77
XhoI C'TCGA_G
Cuts at: 0 181 822
Size: 181 641
XmaI C'CCGG_G
Cuts at: 0 207 822
Size: 207 615
Enzymes that do cut:
AarI AflIII AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BpmI BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI EaeI
EarI EcoICRI Eco57MI FalI HaeII KasI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmlI PspOMI
PspXI PstI PvuII SacI SapI SbfI SfcI SfoI
SmaI SmlI TaqII XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XcmI XmnI ZraI