Restriction map for
pFN21AB5294
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HindIII KasI
KpnI KspI MaeI MfeI MflI
MluI MroI MslI MspA1I MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 565 CGCG CG^CG
AclI 444 AACGTT AA^CGTT
AcyI 83 GACGCC G[GA]^CG[CT]C
AflIII 881 ACACGT A^C[GA][CT]GT
AocI 793 CCTGAGG CC^T[ACGT]AGG
AvaI 384 CTCGAG C^[CT]CG[GA]G
AvaII 396 GGACC G^G[AT]CC
AviII 803 TGCGCA TGC^GCA
BanII 709 GAGCCC G[GA]GC[CT]^C
BcgI 716 GCAACTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1017 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 83 GACGCC G[GA]^CG[CT]C
BseRI 784 GAGGAG GAGGAG(10/8)
BsmFI 885 GTCCC GGGAC(10/14)
BsoBI 384 CTCGAG C^[CT]CG[GA]G
BspDI 895 ATCGAT AT^CGAT
BspHI 983 TCATGA T^CATGA
BspMI 486 GCAGGT ACCTGC(4/8)
BstUI 565 CGCG CG^CG
BstXI 990 CCACTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 793 CCTGAGG CC^T[ACGT]AGG
ClaI 895 ATCGAT AT^CGAT
DsaI 950 CCATGG C^C[GA][CT]GG
EarI 352 GAAGAG CTCTTC(1/4)
Eco81I 793 CCTGAGG CC^T[ACGT]AGG
EcoNI 400 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 950 CCATGG C^C[AT][AT]GG
FspI 803 TGCGCA TGC^GCA
Hin1I 83 GACGCC G[GA]^CG[CT]C
HincII 424 GTTAAC GT[CT]^[GA]AC
HindII 424 GTTAAC GT[CT]^[GA]AC
HinfI 190 GACTC G^A[ACGT]TC
HpaI 424 GTTAAC GTT^AAC
Ksp632I 352 GAAGAG CTCTTC(1/4)
MamI 1017 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 425 TTAA T^TAA
MvnI 565 CGCG CG^CG
NaeI 433 GCCGGC GCC^GGC
NcoI 950 CCATGG C^CATGG
NgoMIV 433 GCCGGC G^CCGGC
PaeR7I 384 CTCGAG C^TCGAG
PflMI 945 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 190 GACTC GAGTC(4/5)
PpuMI 395 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 23 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 444 AACGTT AA^CGTT
RcaI 983 TCATGA T^CATGA
ScaI 824 AGTACT AGT^ACT
SfiI 600 GGCCAACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 384 CTCGAG C^T[CT][GA]AG
SphI 584 GCATGC GCATG^C
StyI 950 CCATGG C^C[AT][AT]GG
Tru9I 425 TTAA T^TAA
Van91I 945 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 384 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BcnI 606 CCCGG CC^[GC]GG
712 CCCGG
BglI 139 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
601 GCCAACCCGGC
BmyI 510 GGGCAC G[AGT]GC[ACT]^C
709 GAGCCC
BsaI 475 GAGACC GGTCTC(1/5)
853 GAGACC
BsmAI 475 GAGAC GTCTC(1/5)
853 GAGAC
Bsp1286I 510 GGGCAC G[AGT]GC[ACT]^C
709 GAGCCC
BsrBI 381 CCGCTC CCGCTC(-3/-3)
612 CCGCTC
BsrFI 153 GCCGGT [GA]^CCGG[CT]
433 GCCGGC
BsrI 962 ACTGG ACTGG(1/-1)
140 CCAGT
CfoI 666 GCGC GCG^C
804 GCGC
Cfr10I 153 GCCGGT [GA]^CCGG[CT]
433 GCCGGC
DraII 395 AGGACCC [GA]G^G[ACGT]CC[CT]
416 GGGGCCT
EcoO109I 395 AGGACCC [GA]G^G[ACGT]CC[CT]
416 GGGGCCT
HgaI 83 GACGC GACGC(5/10)
566 GCGTC
HhaI 666 GCGC GCG^C
804 GCGC
HinP1I 666 GCGC G^CGC
804 GCGC
HphI 464 TCACC GGTGA(8/7)
629 TCACC
MaeII 445 ACGT A^CGT
883 ACGT
MboII 352 GAAGA GAAGA(8/7)
774 GAAGA
NciI 606 CCCGG CC^[GC]GG
712 CCCGG
NspI 410 GCATGT [GA]CATG^[CT]
584 GCATGC
PstI 342 CTGCAG CTGCA^G
861 CTGCAG
SfcI 342 CTGCAG C^T[GA][CT]AG
861 CTGCAG
TaqI 385 TCGA T^CGA
896 TCGA
TthHB8I 385 TCGA T^CGA
896 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BpmI BsaJI BsiYI BslI BsmI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeIII MboI MluNI MnlI
MscI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI