Restriction map for
pFN21AB5294
(Linear) (Six-base) MAPSORT of: KIBB5294.seq Check: 7190 from: 1 to: 1029
KIBB5294.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 1029
Size: 477 552
AclI AA'CG_TT
Cuts at: 0 445 1029
Size: 445 584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1029
Size: 412 617
AflIII A'CryG_T
Cuts at: 0 881 1029
Size: 881 148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 445 477 1029
Size: 445 32 552
Fragments arranged by size:
552 445 32
AvaI C'yCGr_G
Cuts at: 0 384 1029
Size: 384 645
BanII G_rGCy'C
Cuts at: 0 713 1029
Size: 713 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 705 739 1029
Size: 705 34 290
Fragments arranged by size:
705 290 34
BglI GCCn_nnn'nGGC
Cuts at: 0 145 607 1029
Size: 145 462 422
Fragments arranged by size:
462 422 145
Bme1580I G_kGCm'C
Cuts at: 0 514 1029
Size: 514 515
BmgBI CAC'GTC
Cuts at: 0 884 1029
Size: 884 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 1029
Size: 669 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 493 1019 1029
Size: 3 490 526 10
Fragments arranged by size:
526 490 10 3
BsaI GGTCTCn'nnnn_
Cuts at: 0 469 847 1029
Size: 469 378 182
Fragments arranged by size:
469 378 182
BsaBI GATnn'nnATC
Cuts at: 0 1021 1029
Size: 1021 8
BsaHI Gr'CG_yC
Cuts at: 0 84 1029
Size: 84 945
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 1029
Size: 445 30 554
Fragments arranged by size:
554 445 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 1029
Size: 799 230
BseYI C'CCAG_C
Cuts at: 0 588 1029
Size: 588 441
BsmI GAATG_Cn'
Cuts at: 0 225 755 980 1029
Size: 225 530 225 49
Fragments arranged by size:
530 225 225 49
Bsp1286I G_dGCh'C
Cuts at: 0 514 713 1029
Size: 514 199 316
Fragments arranged by size:
514 316 199
BspHI T'CATG_A
Cuts at: 0 983 1029
Size: 983 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 1029
Size: 477 552
BsrBI CCG'CTC
Cuts at: 0 383 614 1029
Size: 383 231 415
Fragments arranged by size:
415 383 231
BsrFI r'CCGG_y
Cuts at: 0 153 433 1029
Size: 153 280 596
Fragments arranged by size:
596 280 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 997 1029
Size: 997 32
Bsu36I CC'TnA_GG
Cuts at: 0 794 1029
Size: 794 235
BtgI C'CryG_G
Cuts at: 0 950 1029
Size: 950 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 708 750 1029
Size: 708 42 279
Fragments arranged by size:
708 279 42
BtsI GCAGTG_nn'
Cuts at: 0 873 1029
Size: 873 156
ClaI AT'CG_AT
Cuts at: 0 896 1029
Size: 896 133
EaeI y'GGCC_r
Cuts at: 0 5 123 212 599 1029
Size: 5 118 89 387 430
Fragments arranged by size:
430 387 118 89 5
EarI CTCTTCn'nnn_
Cuts at: 0 347 1029
Size: 347 682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 1029
Size: 163 866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 412 493 1019 1029
Size: 3 409 81 526 10
Fragments arranged by size:
526 409 81 10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 404 1029
Size: 404 625
EcoO109I rG'GnC_Cy
Cuts at: 0 396 417 1029
Size: 396 21 612
Fragments arranged by size:
612 396 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 520 843 1029
Size: 256 264 323 186
Fragments arranged by size:
323 264 256 186
FspI TGC'GCA
Cuts at: 0 805 1029
Size: 805 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 111 143 1029
Size: 111 32 886
Fragments arranged by size:
886 111 32
HincII GTy'rAC
Cuts at: 0 426 1029
Size: 426 603
HpaI GTT'AAC
Cuts at: 0 426 1029
Size: 426 603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1029
Size: 109 920
MscI TGG'CCA
Cuts at: 0 7 214 601 1029
Size: 7 207 387 428
Fragments arranged by size:
428 387 207 7
NaeI GCC'GGC
Cuts at: 0 435 1029
Size: 435 594
NcoI C'CATG_G
Cuts at: 0 950 1029
Size: 950 79
NgoMIV G'CCGG_C
Cuts at: 0 433 1029
Size: 433 596
NspI r_CATG'y
Cuts at: 0 414 588 1029
Size: 414 174 441
Fragments arranged by size:
441 414 174
PasI CC'CwG_GG
Cuts at: 0 684 1029
Size: 684 345
PflMI CCAn_nnn'nTGG
Cuts at: 0 951 1029
Size: 951 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 1029
Size: 445 32 552
Fragments arranged by size:
552 445 32
PpuMI rG'GwC_Cy
Cuts at: 0 396 1029
Size: 396 633
PshAI GACnn'nnGTC
Cuts at: 0 27 1029
Size: 27 1002
PspXI vC'TCGA_Gb
Cuts at: 0 384 1029
Size: 384 645
PstI C_TGCA'G
Cuts at: 0 346 865 1029
Size: 346 519 164
Fragments arranged by size:
519 346 164
ScaI AGT'ACT
Cuts at: 0 826 1029
Size: 826 203
SfcI C'TryA_G
Cuts at: 0 342 861 1029
Size: 342 519 168
Fragments arranged by size:
519 342 168
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 607 1029
Size: 607 422
SmlI C'TyrA_G
Cuts at: 0 384 1029
Size: 384 645
SphI G_CATG'C
Cuts at: 0 588 1029
Size: 588 441
StyI C'CwwG_G
Cuts at: 0 950 1029
Size: 950 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 329 1029
Size: 329 700
TatI w'GTAC_w
Cuts at: 0 824 1029
Size: 824 205
XhoI C'TCGA_G
Cuts at: 0 384 1029
Size: 384 645
Enzymes that do cut:
AarI AclI AcuI AflIII AloI AvaI BanII BcgI
BglI Bme1580I BmgBI BplI BpmI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsmI Bsp1286I BspHI BspMI BsrBI
BsrFI BstXI Bsu36I BtgI BtgZI BtsI ClaI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI FspI Hin4I
HincII HpaI MmeI MscI NaeI NcoI NgoMIV NspI
PasI PflMI PpiI PpuMI PshAI PspXI PstI ScaI
SfcI SfiI SmlI SphI StyI TaqII TatI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmrI BmtI Bpu10I BpuEI
BsaAI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FseI FspAI HaeII HindIII KasI KpnI MfeI
MluI MslI MspA1I NarI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PsiI PspOMI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI