Restriction map for
pFN21AB9261
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI Ksp632I MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 241 GTAC GT^AC
AlwI 225 GATCC GGATC(4/5)
ApaI 5 GGGCCC GGGCC^C
AvaI 389 CCCGGG C^[CT]CG[GA]G
BanII 5 GGGCCC G[GA]GC[CT]^C
BbsI 310 GAAGAC GAAGAC(2/6)
BbvCI 573 GCTGAGG CCTCAGC(-5/-2)
BmyI 5 GGGCCC G[AGT]GC[ACT]^C
BpmI 220 CTGGAG CTGGAG(16/14)
BpuAI 310 GAAGAC GAAGAC(2/6)
BsgI 487 GTGCAG GTGCAG(16/14)
BsmBI 108 CGTCTC CGTCTC(1/5)
BsoBI 389 CCCGGG C^[CT]CG[GA]G
Bsp1286I 5 GGGCCC G[AGT]GC[ACT]^C
BspMI 479 ACCTGC ACCTGC(4/8)
BsrBI 10 CCGCTC CCGCTC(-3/-3)
BsrFI 82 GCCGGC [GA]^CCGG[CT]
BstYI 224 AGATCC [GA]^GATC[CT]
Cfr10I 82 GCCGGC [GA]^CCGG[CT]
DpnII 225 GATC ^GATC
DrdI 100 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HphI 393 GGTGA GGTGA(8/7)
KspI 192 CCGCGG CCGC^GG
MboI 225 GATC ^GATC
MboII 310 GAAGA GAAGA(8/7)
MflI 224 AGATCC [GA]^GATC[CT]
NaeI 82 GCCGGC GCC^GGC
NdeII 225 GATC ^GATC
NgoMIV 82 GCCGGC G^CCGGC
PflMI 36 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 603 CTGCAG CTGCA^G
RsaI 241 GTAC GT^AC
SacII 192 CCGCGG CCGC^GG
Sau3AI 225 GATC ^GATC
SfcI 603 CTGCAG C^T[GA][CT]AG
SmaI 389 CCCGGG CCC^GGG
TspRI 229 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 36 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 332 CCAAAATAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 224 AGATCC [GA]^GATC[CT]
XmaCI 389 CCCGGG C^CCGGG
XmaI 389 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 28 GGCGCC G[GA]^CG[CT]C
67 GGCGCC
Aor51HI 21 AGCGCT AGC^GCT
581 AGCGCT
AvaII 260 GGACC G^G[AT]CC
591 GGACC
BbeI 28 GGCGCC GGCGC^C
67 GGCGCC
BsaHI 28 GGCGCC G[GA]^CG[CT]C
67 GGCGCC
BsmAI 133 GAGAC GTCTC(1/5)
109 GTCTC
BsrI 257 ACTGG ACTGG(1/-1)
228 CCAGT
DdeI 302 CTCAG C^T[ACGT]AG
574 CTGAG
DraII 4 GGGGCCC [GA]G^G[ACGT]CC[CT]
5 GGGCCCC
DsaI 192 CCGCGG C^C[GA][CT]GG
401 CCACGG
EaeI 84 CGGCCA [CT]^GGCC[GA]
114 CGGCCA
Eco47III 21 AGCGCT AGC^GCT
581 AGCGCT
EcoO109I 4 GGGGCCC [GA]G^G[ACGT]CC[CT]
5 GGGCCCC
HgaI 313 GACGC GACGC(5/10)
428 GCGTC
Hin1I 28 GGCGCC G[GA]^CG[CT]C
67 GGCGCC
KasI 28 GGCGCC G^GCGCC
67 GGCGCC
MaeII 107 ACGT A^CGT
170 ACGT
MspA1I 192 CCGCGG C[AC]G^C[GT]G
198 CAGCGG
NarI 28 GGCGCC GG^CGCC
67 GGCGCC
NlaIII 142 CATG CATG^
147 CATG
SfaNI 298 GCATC GCATC(5/9)
513 GCATC
SfoI 28 GGCGCC GGC^GCC
67 GGCGCC
StuI 473 AGGCCT AGG^CCT
599 AGGCCT
TaqI 283 TCGA T^CGA
434 TCGA
TthHB8I 283 TCGA T^CGA
434 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BcnI BsaJI BsiYI BslI BsmFI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI