Restriction map for
pFN21AB9261
(Linear) (Six-base) MAPSORT of: KIBB9261.seq Check: 284 from: 1 to: 612
KIBB9261.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 612
Size: 423 189
AfeI AGC'GCT
Cuts at: 0 23 583 612
Size: 23 560 29
Fragments arranged by size:
560 29 23
ApaI G_GGCC'C
Cuts at: 0 9 612
Size: 9 603
AvaI C'yCGr_G
Cuts at: 0 389 612
Size: 389 223
BanI G'GyrC_C
Cuts at: 0 28 67 412 458 612
Size: 28 39 345 46 154
Fragments arranged by size:
345 154 46 39 28
BanII G_rGCy'C
Cuts at: 0 9 612
Size: 9 603
BbeI G_GCGC'C
Cuts at: 0 32 71 612
Size: 32 39 541
Fragments arranged by size:
541 39 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 612
Size: 317 295
BbvCI CC'TCA_GC
Cuts at: 0 574 612
Size: 574 38
Bme1580I G_kGCm'C
Cuts at: 0 9 612
Size: 9 603
BmgBI CAC'GTC
Cuts at: 0 108 171 612
Size: 108 63 441
Fragments arranged by size:
441 108 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 612
Size: 241 371
Bpu10I CC'TnA_GC
Cuts at: 0 574 612
Size: 574 38
BsaHI Gr'CG_yC
Cuts at: 0 29 68 612
Size: 29 39 544
Fragments arranged by size:
544 39 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 214 244 612
Size: 94 30 90 30 368
Fragments arranged by size:
368 94 90 30 30
BseYI C'CCAG_C
Cuts at: 0 24 612
Size: 24 588
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 612
Size: 508 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 114 612
Size: 114 498
Bsp1286I G_dGCh'C
Cuts at: 0 9 612
Size: 9 603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 488 612
Size: 488 124
BsrBI CCG'CTC
Cuts at: 0 12 612
Size: 12 600
BsrFI r'CCGG_y
Cuts at: 0 82 612
Size: 82 530
BstYI r'GATC_y
Cuts at: 0 224 612
Size: 224 388
BtgI C'CryG_G
Cuts at: 0 192 401 612
Size: 192 209 211
Fragments arranged by size:
211 209 192
DrdI GACnn_nn'nnGTC
Cuts at: 0 106 612
Size: 106 506
EaeI y'GGCC_r
Cuts at: 0 84 114 612
Size: 84 30 498
Fragments arranged by size:
498 84 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 423 612
Size: 241 182 189
Fragments arranged by size:
241 189 182
EcoO109I rG'GnC_Cy
Cuts at: 0 5 6 612
Size: 5 1 606
Fragments arranged by size:
606 5 1
HaeII r_GCGC'y
Cuts at: 0 25 32 71 585 612
Size: 25 7 39 514 27
Fragments arranged by size:
514 39 27 25 7
KasI G'GCGC_C
Cuts at: 0 28 67 612
Size: 28 39 545
Fragments arranged by size:
545 39 28
MspA1I CmG'CkG
Cuts at: 0 194 200 612
Size: 194 6 412
Fragments arranged by size:
412 194 6
NaeI GCC'GGC
Cuts at: 0 84 612
Size: 84 528
NarI GG'CG_CC
Cuts at: 0 29 68 612
Size: 29 39 544
Fragments arranged by size:
544 39 29
NgoMIV G'CCGG_C
Cuts at: 0 82 612
Size: 82 530
PasI CC'CwG_GG
Cuts at: 0 95 612
Size: 95 517
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 612
Size: 42 570
PspOMI G'GGCC_C
Cuts at: 0 5 612
Size: 5 607
PstI C_TGCA'G
Cuts at: 0 607 612
Size: 607 5
SacII CC_GC'GG
Cuts at: 0 195 612
Size: 195 417
SfcI C'TryA_G
Cuts at: 0 603 612
Size: 603 9
SfoI GGC'GCC
Cuts at: 0 30 69 612
Size: 30 39 543
Fragments arranged by size:
543 39 30
SmaI CCC'GGG
Cuts at: 0 391 612
Size: 391 221
StuI AGG'CCT
Cuts at: 0 475 601 612
Size: 475 126 11
Fragments arranged by size:
475 126 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 612
Size: 277 335
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 612
Size: 214 32 366
Fragments arranged by size:
366 214 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 612
Size: 339 273
XmaI C'CCGG_G
Cuts at: 0 389 612
Size: 389 223
Enzymes that do cut:
AcuI AfeI ApaI AvaI BanI BanII BbeI BbsI
BbvCI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaXI BseYI
BsgI BsmBI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI
DrdI EaeI Eco57MI EcoO109I HaeII KasI MspA1I NaeI
NarI NgoMIV PasI PflMI PspOMI PstI SacII SfcI
SfoI SmaI StuI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI