Restriction map for
pFN21AE1008
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI KpnI MaeI MaeIII MamI
MfeI MluI MroI MseI MslI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 456 GTAC GT^AC
AocI 391 CCTGAGG CC^T[ACGT]AGG
ApaI 155 GGGCCC GGGCC^C
AspHI 244 GAGCTC G[AT]GC[AT]^C
AvaII 262 GGACC G^G[AT]CC
BalI 41 TGGCCA TGG^CCA
BamHI 288 GGATCC G^GATCC
BbvCI 441 GCTGAGG CCTCAGC(-5/-2)
BsgI 583 GTGCAG GTGCAG(16/14)
BsiHKAI 244 GAGCTC G[AT]GC[AT]^C
BsmAI 553 GAGAC GTCTC(1/5)
BsmBI 553 GAGACG CGTCTC(1/5)
BspHI 536 TCATGA T^CATGA
BssHII 116 GCGCGC G^CGCGC
BstF5I 360 GGATG GGATG(2/0)
BstXI 44 CCACCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 288 GGATCC [GA]^GATC[CT]
Bsu36I 391 CCTGAGG CC^T[ACGT]AGG
DpnII 289 GATC ^GATC
EarI 343 GAAGAG CTCTTC(1/4)
Eco81I 391 CCTGAGG CC^T[ACGT]AGG
FokI 360 GGATG GGATG(9/13)
HphI 364 GGTGA GGTGA(8/7)
Ksp632I 343 GAAGAG CTCTTC(1/4)
MboI 289 GATC ^GATC
MflI 288 GGATCC [GA]^GATC[CT]
MluNI 41 TGGCCA TGG^CCA
MscI 41 TGGCCA TGG^CCA
NdeII 289 GATC ^GATC
NotI 91 GCGGCCGC GC^GGCCGC
PvuII 514 CAGCTG CAG^CTG
RcaI 536 TCATGA T^CATGA
RsaI 456 GTAC GT^AC
SacI 244 GAGCTC GAGCT^C
Sau3AI 289 GATC ^GATC
SfiI 147 GGCCACCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 433 CCCGGG CCC^GGG
XcmI 473 CCAAAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 288 GGATCC [GA]^GATC[CT]
XmaCI 433 CCCGGG C^CCGGG
XmaI 433 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AlwI 289 GATCC GGATC(4/5)
288 GGATC
BpmI 221 CTGGAG CTGGAG(16/14)
484 CTGGAG
BsiYI 138 CCCGGCCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
183 CCCCGAGCCGG
BslI 138 CCCGGCCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
183 CCCCGAGCCGG
BsmFI 261 GGGAC GGGAC(10/14)
168 GTCCC
BsrFI 9 GCCGGC [GA]^CCGG[CT]
189 GCCGGC
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
189 GCCGGC
DdeI 392 CTGAG C^T[ACGT]AG
442 CTGAG
MaeII 556 ACGT A^CGT
581 ACGT
NaeI 9 GCCGGC GCC^GGC
189 GCCGGC
NgoMIV 9 GCCGGC G^CCGGC
189 GCCGGC
NlaIII 537 CATG CATG^
609 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsiEI BsoBI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI KspI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIV
SacII Sau96I ScrFI SfoI TseI