Restriction map for
pFN21AE1008
(Circular) (Six-base) MAPSORT of: pFN21AE1008.seq Check: 9437 from: 1 to: 5317
pFN21AE1008 5317 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3127 278
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3380 4764 5032 1632
Size: 1748 1384 268 1917
Fragments arranged by size:
1917 1748 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 4148 4148
Size: 5317
AhdI GACnn_n'nnGTC
Cuts at: 4046 4046
Size: 5317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 3060 4632 3060
Size: 1572 3745
ApaI G_GGCC'C
Cuts at: 2164 2164
Size: 5317
ApaLI G'TGCA_C
Cuts at: 3374 4530 3374
Size: 1156 4161
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 2252 2438 2642 1870
Size: 73 246 63 186 204 4545
Fragments arranged by size:
4545 246 204 186 73 63
BamHI G'GATC_C
Cuts at: 2293 2647 3014 2293
Size: 354 367 4596
Fragments arranged by size:
4596 367 354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2167 2305 2638 4093
Size: 325 217 627 224 156 138 333 1455
Cuts at: 4093 618
Size: 1842
Fragments arranged by size:
1842 1455 627 333 325 224 217 156
138
BanII G_rGCy'C
Cuts at: 1391 2108 2164 2235 2253 2280 2636 1391
Size: 717 56 71 18 27 356 4072
Fragments arranged by size:
4072 717 356 71 56 27 18
BbeI G_GCGC'C
Cuts at: 2015 2171 2309 2015
Size: 156 138 5023
Fragments arranged by size:
5023 156 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4346
BbvCI CC'TCA_GC
Cuts at: 2447 2447
Size: 5317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3508 3542 1903
Size: 34 1571 34 3678
Fragments arranged by size:
3678 1571 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2159 2212
Size: 107 122 71 631 605 337 150 53
Cuts at: 2212 2239 2284 3928 136
Size: 27 45 1644 1525
Fragments arranged by size:
1644 1525 631 605 337 150 122 107
71 53 45 27
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2734 2734
Size: 5317
Bme1580I G_kGCm'C
Cuts at: 1790 2164 3378 4534 1790
Size: 374 1214 1156 2573
Fragments arranged by size:
2573 1214 1156 374
BmgBI CAC'GTC
Cuts at: 1318 2587 1318
Size: 1269 4048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3048 4006 442
Size: 416 53 83 399 24 1631 958 1753
Fragments arranged by size:
1753 1631 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 2510 3977 1239
Size: 1008 263 1467 2579
Fragments arranged by size:
2579 1467 1008 263
Bpu10I CC'TnA_GC
Cuts at: 2447 2447
Size: 5317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3442 4307 4605 3442
Size: 865 298 4154
Fragments arranged by size:
4154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3980 882
Size: 617 2481 2219
Fragments arranged by size:
2481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3108 493
Size: 711 1904 2702
Fragments arranged by size:
2702 1904 711
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2168 2306 3124
Size: 53 83 186 936 479 156 138 818
Cuts at: 3124 3506 4141 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 818 635 479 382 186 156
138 83 53
BsaWI w'CCGG_w
Cuts at: 3750 4148 4422 4569 4980 3750
Size: 398 274 147 411 4087
Fragments arranged by size:
4087 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5092
Fragments arranged by size:
5092 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 2336 2366 2414 2417 2498 2507 2510
Size: 1048 102 30 48 3 81 9 3
Cuts at: 2510 1186
Size: 3993
Fragments arranged by size:
3993 1048 102 81 48 30 9 3
3
BseYI C'CCAG_C
Cuts at: 2242 4520 5016 2242
Size: 2278 496 2543
Fragments arranged by size:
2543 2278 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 1163
Size: 573 873 3871
Fragments arranged by size:
3871 873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2100 2148 2767 3528 4556
Size: 275 186 18 81 48 619 761 1028
Cuts at: 4556 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 619 275 186 81 48
18
BsiHKAI G_wGCw'C
Cuts at: 2253 3378 3463 4534 2253
Size: 1125 85 1071 3036
Fragments arranged by size:
3036 1125 1071 85
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
BsmBI CGTCTCn'nnnn_
Cuts at: 2552 2552
Size: 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2164 2235 2253 2280 2636 3378
Size: 399 318 56 71 18 27 356 742
Cuts at: 3378 3463 4534 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 318 85 71
56 27 18
BspEI T'CCGG_A
Cuts at: 4980 4980
Size: 5317
BspHI T'CATG_A
Cuts at: 2541 3206 4936 2541
Size: 665 1730 2922
Fragments arranged by size:
2922 1730 665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2672 844
Size: 1828 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3204 1394
Size: 429 1381 3507
Fragments arranged by size:
3507 1381 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3812 3986 65
Size: 1183 2564 174 1396
Fragments arranged by size:
2564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2194 3961 4148 1700
Size: 110 204 180 1767 187 2869
Fragments arranged by size:
2869 1767 204 187 180 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 2121 1925
Size: 196 5121
BssSI C'ACGA_G
Cuts at: 3377 4389 3377
Size: 1012 4305
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5317
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5317
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 5317
BstYI r'GATC_y
Cuts at: 1972 2293 2647 3014 3399 3416 4868 4954 4966
Size: 321 354 367 385 17 1452 86 12
Cuts at: 4966 5312 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 321 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2397 2397
Size: 5317
BtgI C'CryG_G
Cuts at: 513 1065 2004 2208 2235 2298 513
Size: 552 939 204 27 63 3532
Fragments arranged by size:
3532 939 552 204 63 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4578
Fragments arranged by size:
4578 683 56
BtsI GCAGTG_nn'
Cuts at: 2833 3627 3647 2833
Size: 794 20 4503
Fragments arranged by size:
4503 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5282
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4324 809
Size: 507 3008 1802
Fragments arranged by size:
3008 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2028 2046
Size: 54 1425 50 275 18 186 12 18
Cuts at: 2046 2097 2145 2764 3653 5282 8
Size: 51 48 619 889 1629 43
Fragments arranged by size:
1629 1425 889 619 275 186 54 51
50 48 43 18 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2097 2145 2764 1537
Size: 275 204 81 48 619 4090
Fragments arranged by size:
4090 619 275 204 81 48
EarI CTCTTCn'nnn_
Cuts at: 1353 2343 3247 1353
Size: 990 904 3423
Fragments arranged by size:
3423 990 904
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2333 3904 4278 4424 387
Size: 1064 138 744 1571 374 146 1280
Fragments arranged by size:
1571 1280 1064 744 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2251
Size: 5317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2510 3380 3977 4764 5032 1239
Size: 393 615 263 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 615 597 393 268 263
EcoO109I rG'GnC_Cy
Cuts at: 2160 2161 2393 2160
Size: 1 232 5084
Fragments arranged by size:
5084 232 1
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FspI TGC'GCA
Cuts at: 3823 3823
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2015 2171 2309 4464 1678
Size: 337 156 138 2155 2531
Fragments arranged by size:
2531 2155 337 156 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373
Size: 32 135 32 2386 32 42 32 2626
Fragments arranged by size:
2626 2386 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2661 2907 669
Size: 988 281 723 246 3079
Fragments arranged by size:
3079 988 723 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2907 2907
Size: 5317
KasI G'GCGC_C
Cuts at: 2011 2167 2305 2011
Size: 156 138 5023
Fragments arranged by size:
5023 156 138
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 4431 4615 1305
Size: 1296 1830 184 2007
Fragments arranged by size:
2007 1830 1296 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 10
Size: 54 1425 343 216 3279
Fragments arranged by size:
3279 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 3634 3793 518
Size: 648 2109 359 159 2042
Fragments arranged by size:
2109 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 2237 2300 2312 2521 2733 3410 4558
Size: 604 27 63 12 209 212 677 1148
Cuts at: 4558 4803 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 604 245 212 209 63
27 12
NaeI GCC'GGC
Cuts at: 2016 2196 2016
Size: 180 5137
NarI GG'CG_CC
Cuts at: 2012 2168 2306 2012
Size: 156 138 5023
Fragments arranged by size:
5023 156 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3826
Fragments arranged by size:
3826 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NgoMIV G'CCGG_C
Cuts at: 2014 2194 2014
Size: 180 5137
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5317
NotI GC'GGCC_GC
Cuts at: 2097 2764 2097
Size: 667 4650
NspI r_CATG'y
Cuts at: 1132 1229 2675 4220 1132
Size: 97 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5317
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5317
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 4899 4931 3386
Size: 32 1481 32 3772
Fragments arranged by size:
3772 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PspOMI G'GGCC_C
Cuts at: 2160 2160
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2669 830
Size: 1839 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
PvuII CAG'CTG
Cuts at: 2521 2521
Size: 5317
SacI G_AGCT'C
Cuts at: 2253 2253
Size: 5317
SacII CC_GC'GG
Cuts at: 2211 2238 2301 2211
Size: 27 63 5227
Fragments arranged by size:
5227 63 27
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SbfI CC_TGCA'GG
Cuts at: 2669 2669
Size: 5317
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2665 3800 4481 4672 826
Size: 219 1620 1135 681 191 1471
Fragments arranged by size:
1620 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2159 2159
Size: 5317
SfoI GGC'GCC
Cuts at: 2013 2169 2307 2013
Size: 156 138 5023
Fragments arranged by size:
5023 156 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2440 2644 1872
Size: 568 204 4545
Fragments arranged by size:
4545 568 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3421 4322 4584 820
Size: 197 926 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2675 1132
Size: 1543 3774
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5317
StyI C'CwwG_G
Cuts at: 513 1065 2004 2756 513
Size: 552 939 752 3074
Fragments arranged by size:
3074 939 752 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3344 3361 3514 3699 4144 325
Size: 1255 1764 17 153 185 445 1498
Fragments arranged by size:
1764 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 3563 5275
Size: 274 80 33 51 1443 483 1103 1712
Cuts at: 5275 96
Size: 138
Fragments arranged by size:
1712 1443 1103 483 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5277 5309 5277
Size: 32 5285
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2485
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2438 2642 1870
Size: 568 204 4545
Fragments arranged by size:
4545 568 204
XmnI GAAnn'nnTTC
Cuts at: 3446 3446
Size: 5317
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3125 276
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SanDI SapI SexAI SrfI SwaI