################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MroI MseI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 62 GCGG CCGC(-3/-1) AcsI 127 AAATTT [GA]^AATT[CT] AfaI 8 GTAC GT^AC AluI 152 AGCT AG^CT ApoI 127 AAATTT [GA]^AATT[CT] AviII 217 TGCGCA TGC^GCA BalI 155 TGGCCA TGG^CCA BciVI 100 GGATAC GTATCC(6/5) BseRI 259 CTCCTC GAGGAG(10/8) BsmAI 257 GTCTC GTCTC(1/5) BsmBI 256 CGTCTC CGTCTC(1/5) BssKI 24 CCTGG ^CC[ACGT]GG BstF5I 121 CATCC GGATG(2/0) BstNI 24 CCTGG CC^[AT]GG CfoI 218 GCGC GCG^C DdeI 262 CTCAG C^T[ACGT]AG EaeI 155 TGGCCA [CT]^GGCC[GA] EcoRII 24 CCTGG ^CC[AT]GG FokI 121 CATCC GGATG(9/13) FspI 217 TGCGCA TGC^GCA HaeIII 156 GGCC GG^CC HgaI 255 GCGTC GACGC(5/10) HhaI 218 GCGC GCG^C HinP1I 218 GCGC G^CGC HinfI 67 GAATC G^A[ACGT]TC HphI 240 GGTGA GGTGA(8/7) MaeIII 170 GTGAC ^GT[ACGT]AC MboII 117 TCTTC GAAGA(8/7) MluNI 155 TGGCCA TGG^CCA MscI 155 TGGCCA TGG^CCA MspA1I 151 CAGCTG C[AC]G^C[GT]G MvaI 24 CCTGG CC^[AT]GG NlaIII 282 CATG CATG^ PshAI 88 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 151 CAGCTG CAG^CTG RsaI 8 GTAC GT^AC ScrFI 24 CCTGG CC^[ACGT]GG TfiI 67 GAATC G^A[AT]TC Tsp45I 170 GTGAC ^GT[GC]AC Tsp509I 128 AATT ^AATT TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 226 CCAAAACAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 2 enzymes) # ################################### Cac8I 76 GCAAGC GC[ACGT]^[ACGT]GC 153 GCTGGC MnlI 57 CCTC CCTC(7/6) 261 CCTC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BbvI Fnu4HI ItaI TseI