################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeI MaeII MamI MboII MfeI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 84 CGCG CG^CG AlwI 79 GATCC GGATC(4/5) AlwNI 250 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 161 CCCGAG C^[CT]CG[GA]G AvaII 258 GGACC G^G[AT]CC BanII 87 GGGCTC G[GA]GC[CT]^C BbvCI 129 GCTGAGG CCTCAGC(-5/-2) BmyI 87 GGGCTC G[AGT]GC[ACT]^C BsiYI 263 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 263 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 161 CCCGAG C^[CT]CG[GA]G Bsp1286I 87 GGGCTC G[AGT]GC[ACT]^C BspMI 245 ACCTGC ACCTGC(4/8) BsrFI 63 GCCGGT [GA]^CCGG[CT] BsrI 39 ACTGG ACTGG(1/-1) BstAPI 60 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 84 CGCG CG^CG BstYI 78 AGATCC [GA]^GATC[CT] Cfr10I 63 GCCGGT [GA]^CCGG[CT] DdeI 130 CTGAG C^T[ACGT]AG DpnII 79 GATC ^GATC DsaI 83 CCGCGG C^C[GA][CT]GG EaeI 309 TGGCCG [CT]^GGCC[GA] HgaI 57 GACGC GACGC(5/10) HinfI 157 GAGTC G^A[ACGT]TC KspI 83 CCGCGG CCGC^GG MaeIII 278 GTGAC ^GT[ACGT]AC MboI 79 GATC ^GATC MflI 78 AGATCC [GA]^GATC[CT] MseI 239 TTAA T^TAA MvnI 84 CGCG CG^CG NdeII 79 GATC ^GATC NlaIII 291 CATG CATG^ PleI 157 GAGTC GAGTC(4/5) PshAI 224 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 127 CAGCTG CAG^CTG SacII 83 CCGCGG CCGC^GG Sau3AI 79 GATC ^GATC Tru9I 239 TTAA T^TAA Tsp45I 278 GTGAC ^GT[GC]AC TspRI 318 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 78 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 37 GTAC GT^AC 336 GTAC AluI 128 AGCT AG^CT 213 AGCT BglI 117 GCCCCGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 178 GCCCTGCTGGC BseRI 109 GAGGAG GAGGAG(10/8) 325 GAGGAG BsmFI 284 GGGAC GGGAC(10/14) 159 GTCCC BstNI 171 CCTGG CC^[AT]GG 255 CCTGG Cfr13I 204 GGCCC G^G[ACGT]CC 258 GGACC EcoRII 171 CCTGG ^CC[AT]GG 255 CCTGG HaeIII 204 GGCC GG^CC 310 GGCC MvaI 171 CCTGG CC^[AT]GG 255 CCTGG PstI 97 CTGCAG CTGCA^G 247 CTGCAG RsaI 37 GTAC GT^AC 336 GTAC Sau96I 204 GGCCC G^G[ACGT]CC 258 GGACC SfcI 97 CTGCAG C^T[GA][CT]AG 247 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AcyI BanI BbeI BbvI BcnI BsaHI BsaJI BssKI BstF5I Cac8I CfoI FauI Fnu4HI FokI HaeII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MwoI NarI NciI NlaIV ScrFI SfoI TseI