(Circular) (Six-base) MAPSORT of: pFN21AE3612.seq Check: 3346 from: 1 to: 5050 pFN21AE3612 5050 bp With 182 enzymes: SgfI * March 28, 2012 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2860 278 Size: 53 83 186 936 1324 2468 Fragments arranged by size: 2468 1324 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2393 1937 Size: 456 4594 Acc65I G'GTAC_C Cuts at: 2371 2371 Size: 5050 AclI AA'CG_TT Cuts at: 1563 3177 3550 1563 Size: 1614 373 3063 Fragments arranged by size: 3063 1614 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3113 4497 4765 1632 Size: 1481 1384 268 1917 Fragments arranged by size: 1917 1481 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4853 AflIII A'CryG_T Cuts at: 1203 3949 4926 1203 Size: 2746 977 1327 Fragments arranged by size: 2746 1327 977 AgeI A'CCGG_T Cuts at: 3881 3881 Size: 5050 AhdI GACnn_n'nnGTC Cuts at: 3779 3779 Size: 5050 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5050 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5050 AlwNI CAG_nnn'CTG Cuts at: 2260 2793 4365 2260 Size: 533 1572 2945 Fragments arranged by size: 2945 1572 533 ApaLI G'TGCA_C Cuts at: 3107 4263 3107 Size: 1156 3894 ApoI r'AATT_y Cuts at: 1493 2359 2585 2737 4846 1493 Size: 866 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 226 152 AseI AT'TA_AT Cuts at: 160 1032 3604 160 Size: 872 2572 1606 Fragments arranged by size: 2572 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5050 AvaI C'yCGr_G Cuts at: 1870 1943 2166 2375 1870 Size: 73 223 209 4545 Fragments arranged by size: 4545 223 209 73 BamHI G'GATC_C Cuts at: 2380 2747 2380 Size: 367 4683 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2071 2119 2180 2371 Size: 325 217 627 267 17 48 61 191 Cuts at: 2371 3826 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 267 217 191 61 48 17 BanII G_rGCy'C Cuts at: 1391 2096 2369 1391 Size: 705 273 4072 Fragments arranged by size: 4072 705 273 BbeI G_GCGC'C Cuts at: 2058 2123 2184 2058 Size: 65 61 4924 Fragments arranged by size: 4924 65 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4079 BbvCI CC'TCA_GC Cuts at: 2135 2135 Size: 5050 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3241 3275 1903 Size: 34 1304 34 3678 Fragments arranged by size: 3678 1304 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2942 4152 2942 Size: 1210 3840 BclI T'GATC_A Cuts at: 1548 1548 Size: 5050 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2128 2189 3661 Size: 107 122 71 631 605 456 61 1472 Cuts at: 3661 136 Size: 1525 Fragments arranged by size: 1525 1472 631 605 456 122 107 71 61 BglII A'GATC_T Cuts at: 5045 5045 Size: 5050 BlpI GC'TnA_GC Cuts at: 2467 2467 Size: 5050 Bme1580I G_kGCm'C Cuts at: 1790 3111 4267 1790 Size: 1321 1156 2573 Fragments arranged by size: 2573 1321 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5050 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2781 3739 442 Size: 416 53 83 399 24 1364 958 1753 Fragments arranged by size: 1753 1364 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5050 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2362 2362 Size: 5050 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3710 1239 Size: 2471 2579 Bpu10I CC'TnA_GC Cuts at: 2135 2135 Size: 5050 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3175 4040 4338 3175 Size: 865 298 3887 Fragments arranged by size: 3887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3713 882 Size: 617 2214 2219 Fragments arranged by size: 2219 2214 617 BsaAI yAC'GTr Cuts at: 493 1204 2841 493 Size: 711 1637 2702 Fragments arranged by size: 2702 1637 711 BsaBI GATnn'nnATC Cuts at: 5044 5044 Size: 5050 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2120 2181 2857 Size: 53 83 186 936 522 65 61 676 Cuts at: 2857 3239 3874 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 676 635 522 382 186 83 65 61 53 BsaWI w'CCGG_w Cuts at: 3483 3881 4155 4302 4713 3483 Size: 398 274 147 411 3820 Fragments arranged by size: 3820 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2354 1178 Size: 30 165 30 921 30 3874 Fragments arranged by size: 3874 921 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2345 1186 Size: 943 216 3891 Fragments arranged by size: 3891 943 216 BseYI C'CCAG_C Cuts at: 2267 4253 4749 2267 Size: 1986 496 2568 Fragments arranged by size: 2568 1986 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4477 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2500 3261 4289 1540 Size: 275 186 499 761 1028 2301 Fragments arranged by size: 2301 1028 761 499 275 186 BsiHKAI G_wGCw'C Cuts at: 3111 3196 4267 3111 Size: 85 1071 3894 Fragments arranged by size: 3894 1071 85 BsmI GAATG_Cn' Cuts at: 2561 2654 2561 Size: 93 4957 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2369 3111 3196 4267 1391 Size: 399 306 273 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 273 85 BspEI T'CCGG_A Cuts at: 4713 4713 Size: 5050 BspHI T'CATG_A Cuts at: 2939 4669 2939 Size: 1730 3320 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2405 844 Size: 1415 146 3489 Fragments arranged by size: 3489 1415 146 BsrBI CCG'CTC Cuts at: 1394 1823 2937 1394 Size: 429 1114 3507 Fragments arranged by size: 3507 1114 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3545 3719 65 Size: 1183 2297 174 1396 Fragments arranged by size: 2297 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 3694 3881 1700 Size: 110 258 1626 187 2869 Fragments arranged by size: 2869 1626 258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5050 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5050 BssSI C'ACGA_G Cuts at: 3110 4122 3110 Size: 1012 4038 BstAPI GCAn_nnn'nTGC Cuts at: 794 2071 794 Size: 1277 3773 BstBI TT'CG_AA Cuts at: 3854 3854 Size: 5050 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5050 BstYI r'GATC_y Cuts at: 1972 2083 2380 2747 3132 3149 4601 4687 4699 Size: 111 297 367 385 17 1452 86 12 Cuts at: 4699 5045 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 297 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2088 513 Size: 552 1023 3475 Fragments arranged by size: 3475 1023 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4311 Fragments arranged by size: 4311 683 56 BtsI GCAGTG_nn' Cuts at: 2329 2566 3360 3380 2329 Size: 237 794 20 3999 Fragments arranged by size: 3999 794 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5050 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5015 DraI TTT'AAA Cuts at: 2354 2354 Size: 5050 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4057 809 Size: 507 2741 1802 Fragments arranged by size: 2741 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2314 2497 3386 Size: 54 1425 50 275 18 484 183 889 Cuts at: 3386 5015 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 484 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2497 1537 Size: 275 685 4090 Fragments arranged by size: 4090 685 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2980 1353 Size: 1627 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3637 4011 4157 387 Size: 1064 138 2048 374 146 1280 Fragments arranged by size: 2048 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3113 3710 4497 4765 1239 Size: 393 1481 597 787 268 1524 Fragments arranged by size: 1524 1481 787 597 393 268 EcoRI G'AATT_C Cuts at: 2359 2737 2359 Size: 378 4672 FspI TGC'GCA Cuts at: 3556 3556 Size: 5050 HaeII r_GCGC'y Cuts at: 1678 2058 2123 2184 4197 1678 Size: 380 65 61 2013 2531 Fragments arranged by size: 2531 2013 380 65 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 3691 3723 3765 Size: 32 135 32 575 32 1512 32 42 Cuts at: 3765 3797 1373 Size: 32 2626 Fragments arranged by size: 2626 1512 575 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2394 2640 669 Size: 988 281 456 246 3079 Fragments arranged by size: 3079 988 456 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5050 HpaI GTT'AAC Cuts at: 2640 2640 Size: 5050 KasI G'GCGC_C Cuts at: 2054 2119 2180 2054 Size: 65 61 4924 Fragments arranged by size: 4924 65 61 KpnI G_GTAC'C Cuts at: 2375 2375 Size: 5050 MfeI C'AATT_G Cuts at: 2649 2649 Size: 5050 MluI A'CGCG_T Cuts at: 4926 4926 Size: 5050 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4164 4348 1305 Size: 2859 184 2007 Fragments arranged by size: 2859 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3228 Fragments arranged by size: 3228 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3008 3367 3526 518 Size: 648 1842 359 159 2042 Fragments arranged by size: 2042 1842 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2090 2107 2134 2466 3143 4291 4536 Size: 407 77 17 27 332 677 1148 245 Cuts at: 4536 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 407 332 245 77 27 17 NarI GG'CG_CC Cuts at: 2055 2120 2181 2055 Size: 65 61 4924 Fragments arranged by size: 4924 65 61 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4498 NdeI CA'TA_TG Cuts at: 387 387 Size: 5050 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5050 NotI GC'GGCC_GC Cuts at: 2497 2497 Size: 5050 NspI r_CATG'y Cuts at: 1132 1229 2408 3953 1132 Size: 97 1179 1545 2229 Fragments arranged by size: 2229 1545 1179 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5050 PciI A'CATG_T Cuts at: 3949 3949 Size: 5050 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5050 PmeI GTTT'AAAC Cuts at: 2354 2354 Size: 5050 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3119 3151 4632 4664 3119 Size: 32 1481 32 3505 Fragments arranged by size: 3505 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2233 1613 Size: 620 4430 PsiI TTA'TAA Cuts at: 2620 2620 Size: 5050 PstI C_TGCA'G Cuts at: 830 2106 2256 2402 830 Size: 1276 150 146 3478 Fragments arranged by size: 3478 1276 150 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5050 PvuII CAG'CTG Cuts at: 2134 2134 Size: 5050 SacII CC_GC'GG Cuts at: 2091 2091 Size: 5050 SalI G'TCGA_C Cuts at: 1936 2392 1936 Size: 456 4594 SbfI CC_TGCA'GG Cuts at: 2402 2402 Size: 5050 ScaI AGT'ACT Cuts at: 3298 5010 3298 Size: 1712 3338 SfcI C'TryA_G Cuts at: 826 1045 2102 2252 2398 3533 4214 4405 826 Size: 219 1057 150 146 1135 681 191 1471 Fragments arranged by size: 1471 1135 1057 681 219 191 150 146 SfoI GGC'GCC Cuts at: 2056 2121 2182 2056 Size: 65 61 4924 Fragments arranged by size: 4924 65 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5050 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5050 SmaI CCC'GGG Cuts at: 1872 2377 1872 Size: 505 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3154 4055 4317 820 Size: 197 926 1211 901 262 1553 Fragments arranged by size: 1553 1211 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2841 493 Size: 2348 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5050 SphI G_CATG'C Cuts at: 1132 2408 1132 Size: 1276 3774 SspI AAT'ATT Cuts at: 5 52 2974 5 Size: 47 2922 2081 Fragments arranged by size: 2922 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5050 StyI C'CwwG_G Cuts at: 513 1065 2489 513 Size: 552 1424 3074 Fragments arranged by size: 3074 1424 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3077 3094 3247 3432 3877 325 Size: 1255 1497 17 153 185 445 1498 Fragments arranged by size: 1498 1497 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3296 5008 96 Size: 274 80 33 51 1443 1319 1712 138 Fragments arranged by size: 1712 1443 1319 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5010 5042 5010 Size: 32 5018 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5050 XbaI T'CTAG_A Cuts at: 2386 2386 Size: 5050 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5050 XmaI C'CCGG_G Cuts at: 1870 2375 1870 Size: 505 4545 XmnI GAAnn'nnTTC Cuts at: 3179 3179 Size: 5050 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2858 276 Size: 53 83 186 936 1324 2468 Fragments arranged by size: 2468 1324 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI