(Circular) (Six-base) MAPSORT of: pFN21AB5797.seq Check: 8537 from: 1 to: 7207 pFN21AB5797 7207 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4291 4291 Size: 7207 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2532 5017 278 Size: 53 83 186 936 996 2485 2468 Fragments arranged by size: 2485 2468 996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2499 2568 4550 1937 Size: 562 69 1982 4594 Fragments arranged by size: 4594 1982 562 69 Acc65I G'GTAC_C Cuts at: 4528 4528 Size: 7207 AclI AA'CG_TT Cuts at: 1563 4502 5334 5707 1563 Size: 2939 832 373 3063 Fragments arranged by size: 3063 2939 832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3227 3401 3658 3722 4177 5270 6654 6922 Size: 1595 174 257 64 455 1093 1384 268 Cuts at: 6922 1632 Size: 1917 Fragments arranged by size: 1917 1595 1384 1093 455 268 257 174 64 AfeI AGC'GCT Cuts at: 4481 4481 Size: 7207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7010 AflIII A'CryG_T Cuts at: 1203 3141 6106 7083 1203 Size: 1938 2965 977 1327 Fragments arranged by size: 2965 1938 1327 977 AgeI A'CCGG_T Cuts at: 2869 3591 6038 2869 Size: 722 2447 4038 Fragments arranged by size: 4038 2447 722 AhdI GACnn_n'nnGTC Cuts at: 5936 5936 Size: 7207 AleI CACnn'nnGTG Cuts at: 1166 3356 1166 Size: 2190 5017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2229 4323 1184 Size: 1045 2094 4068 Fragments arranged by size: 4068 2094 1045 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3429 3461 3429 Size: 32 7175 AlwNI CAG_nnn'CTG Cuts at: 2861 2893 3499 3769 4126 4950 6522 2861 Size: 32 606 270 357 824 1572 3546 Fragments arranged by size: 3546 1572 824 606 357 270 32 ApaI G_GGCC'C Cuts at: 2448 2448 Size: 7207 ApaLI G'TGCA_C Cuts at: 4260 4436 5264 6420 4260 Size: 176 828 1156 5047 Fragments arranged by size: 5047 1156 828 176 ApoI r'AATT_y Cuts at: 1493 2352 4516 4742 4894 7003 1493 Size: 859 2164 226 152 2109 1697 Fragments arranged by size: 2164 2109 1697 859 226 152 AseI AT'TA_AT Cuts at: 160 1032 5761 160 Size: 872 4729 1606 Fragments arranged by size: 4729 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 AvaI C'yCGr_G Cuts at: 1870 1943 2307 2964 3078 4532 1870 Size: 73 364 657 114 1454 4545 Fragments arranged by size: 4545 1454 657 364 114 73 BamHI G'GATC_C Cuts at: 3217 4537 4904 3217 Size: 1320 367 5520 Fragments arranged by size: 5520 1320 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2378 2404 2883 3616 4528 Size: 325 217 627 591 26 479 733 912 Cuts at: 4528 5983 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 912 733 627 591 479 325 217 26 BanII G_rGCy'C Cuts at: 1391 2448 2939 4526 1391 Size: 1057 491 1587 4072 Fragments arranged by size: 4072 1587 1057 491 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3144 3489 928 Size: 971 1245 345 4646 Fragments arranged by size: 4646 1245 971 345 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2216 2250 5398 5432 1903 Size: 34 279 34 3148 34 3678 Fragments arranged by size: 3678 3148 279 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5099 6309 5099 Size: 1210 5997 BclI T'GATC_A Cuts at: 1548 1548 Size: 7207 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5818 136 Size: 107 122 71 631 605 4146 1525 Fragments arranged by size: 4146 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7202 7202 Size: 7207 BlpI GC'TnA_GC Cuts at: 4001 4207 4624 4001 Size: 206 417 6584 Fragments arranged by size: 6584 417 206 Bme1580I G_kGCm'C Cuts at: 1790 2448 3621 4264 4440 5268 6424 1790 Size: 658 1173 643 176 828 1156 2573 Fragments arranged by size: 2573 1173 1156 828 658 643 176 BmgBI CAC'GTC Cuts at: 1318 3150 1318 Size: 1832 5375 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4938 5896 442 Size: 416 53 83 399 24 3521 958 1753 Fragments arranged by size: 3521 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 7207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2203 3100 3236 3262 4324 4511 5867 1239 Size: 964 897 136 26 1062 187 1356 2579 Fragments arranged by size: 2579 1356 1062 964 897 187 136 26 Bpu10I CC'TnA_GC Cuts at: 2711 3195 3824 4110 2711 Size: 484 629 286 5808 Fragments arranged by size: 5808 629 484 286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2544 2619 3002 3284 4349 5332 6197 6495 2544 Size: 75 383 282 1065 983 865 298 3256 Fragments arranged by size: 3256 1065 983 865 383 298 282 75 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 5870 882 Size: 617 2646 1725 2219 Fragments arranged by size: 2646 2219 1725 617 BsaAI yAC'GTr Cuts at: 493 1204 2452 2497 4998 493 Size: 711 1248 45 2501 2702 Fragments arranged by size: 2702 2501 1248 711 45 BsaBI GATnn'nnATC Cuts at: 7201 7201 Size: 7207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2529 3627 5014 5396 Size: 53 83 186 936 996 1098 1387 382 Cuts at: 5396 6031 275 Size: 635 1451 Fragments arranged by size: 1451 1387 1098 996 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2773 2869 3591 5640 6038 6312 6459 6870 2773 Size: 96 722 2049 398 274 147 411 3110 Fragments arranged by size: 3110 2049 722 411 398 274 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3429 3459 3737 3767 1178 Size: 30 165 30 2026 30 278 30 4618 Fragments arranged by size: 4618 2026 278 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3151 3886 3932 1186 Size: 1965 735 46 4461 Fragments arranged by size: 4461 1965 735 46 BseYI C'CCAG_C Cuts at: 3190 4212 4409 4467 6410 6906 3190 Size: 1022 197 58 1943 496 3491 Fragments arranged by size: 3491 1943 1022 496 197 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2438 2788 3550 1163 Size: 573 702 350 762 4820 Fragments arranged by size: 4820 762 702 573 350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2155 4657 5418 6446 1540 Size: 275 186 154 2502 761 1028 2301 Fragments arranged by size: 2502 2301 1028 761 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2144 4111 4264 4440 5268 5353 6424 2144 Size: 1967 153 176 828 85 1071 2927 Fragments arranged by size: 2927 1967 1071 828 176 153 85 BsmI GAATG_Cn' Cuts at: 4718 4811 4718 Size: 93 7114 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2448 2939 3621 4111 4264 4440 Size: 399 354 304 491 682 490 153 176 Cuts at: 4440 4526 5268 5353 6424 1391 Size: 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 682 491 490 399 354 304 176 153 86 85 BspEI T'CCGG_A Cuts at: 6870 6870 Size: 7207 BspHI T'CATG_A Cuts at: 3723 3963 4332 5096 6826 3723 Size: 240 369 764 1730 4104 Fragments arranged by size: 4104 1730 764 369 240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 4291 4562 844 Size: 1966 1481 271 3489 Fragments arranged by size: 3489 1966 1481 271 BsrBI CCG'CTC Cuts at: 1394 1823 5094 1394 Size: 429 3271 3507 Fragments arranged by size: 3507 3271 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5702 5876 65 Size: 1183 4454 174 1396 Fragments arranged by size: 4454 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2869 3591 5851 6038 1700 Size: 110 1059 722 2260 187 2869 Fragments arranged by size: 2869 2260 1059 722 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7207 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7207 BssSI C'ACGA_G Cuts at: 2225 5267 6279 2225 Size: 3042 1012 3153 Fragments arranged by size: 3153 3042 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3422 794 Size: 2628 4579 BstBI TT'CG_AA Cuts at: 6011 6011 Size: 7207 BstEII G'GTnAC_C Cuts at: 1184 3779 1184 Size: 2595 4612 BstXI CCAn_nnnn'nTGG Cuts at: 3286 4378 3286 Size: 1092 6115 BstYI r'GATC_y Cuts at: 1972 3217 3495 4537 4904 5289 5306 6758 6844 Size: 1245 278 1042 367 385 17 1452 86 Cuts at: 6844 6856 7202 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1245 1042 385 367 346 278 86 17 12 BstZ17I GTA'TAC Cuts at: 2500 2569 2500 Size: 69 7138 BtgI C'CryG_G Cuts at: 513 1065 2374 2488 4041 4416 513 Size: 552 1309 114 1553 375 3304 Fragments arranged by size: 3304 1553 1309 552 375 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6468 Fragments arranged by size: 6468 683 56 BtsI GCAGTG_nn' Cuts at: 3363 3809 3862 4723 5517 5537 3363 Size: 446 53 861 794 20 5033 Fragments arranged by size: 5033 861 794 446 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7207 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4023 4058 529 Size: 35 3459 35 3678 Fragments arranged by size: 3678 3459 35 35 DraI TTT'AAA Cuts at: 4511 4511 Size: 7207 DraIII CAC_nnn'GTG Cuts at: 3749 4444 3749 Size: 695 6512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6214 809 Size: 507 4898 1802 Fragments arranged by size: 4898 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2263 2371 3552 Size: 54 1425 50 275 18 433 108 1181 Cuts at: 3552 4392 4654 5543 7172 8 Size: 840 262 889 1629 43 Fragments arranged by size: 1629 1425 1181 889 840 433 275 262 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4654 1537 Size: 275 2842 4090 Fragments arranged by size: 4090 2842 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3215 3677 4196 5137 1353 Size: 1862 462 519 941 3423 Fragments arranged by size: 3423 1862 941 519 462 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2670 5794 6168 6314 387 Size: 1064 138 1081 3124 374 146 1280 Fragments arranged by size: 3124 1280 1081 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 3100 3227 3236 3262 3401 3658 Size: 393 571 897 127 9 26 139 257 Cuts at: 3658 3722 4177 4324 4511 5270 5867 6654 6922 Size: 64 455 147 187 759 597 787 268 Cuts at: 6922 1239 Size: 1524 Fragments arranged by size: 1524 897 787 759 597 571 455 393 268 257 187 147 139 127 64 26 9 EcoNI CCTnn'n_nnAGG Cuts at: 2661 3827 2661 Size: 1166 6041 EcoO109I rG'GnC_Cy Cuts at: 2031 2445 2597 2824 3259 3667 3838 2031 Size: 414 152 227 435 408 171 5400 Fragments arranged by size: 5400 435 414 408 227 171 152 EcoRI G'AATT_C Cuts at: 4516 4894 4516 Size: 378 6829 EcoRV GAT'ATC Cuts at: 2539 2539 Size: 7207 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2726 2726 Size: 7207 FspI TGC'GCA Cuts at: 5713 5713 Size: 7207 HaeII r_GCGC'y Cuts at: 1678 4483 6354 1678 Size: 2805 1871 2531 Fragments arranged by size: 2805 2531 1871 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2301 2333 2495 2527 2617 Size: 32 135 32 729 32 162 32 90 Cuts at: 2617 2649 3487 3519 5848 5880 5922 5954 1373 Size: 32 838 32 2329 32 42 32 2626 Fragments arranged by size: 2626 2329 838 729 162 135 90 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4551 4797 669 Size: 988 281 2613 246 3079 Fragments arranged by size: 3079 2613 988 281 246 HindIII A'AGCT_T Cuts at: 748 2186 748 Size: 1438 5769 HpaI GTT'AAC Cuts at: 4797 4797 Size: 7207 KpnI G_GTAC'C Cuts at: 4532 4532 Size: 7207 MfeI C'AATT_G Cuts at: 4806 4806 Size: 7207 MluI A'CGCG_T Cuts at: 7083 7083 Size: 7207 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2822 3999 6321 6505 1305 Size: 1517 1177 2322 184 2007 Fragments arranged by size: 2322 2007 1517 1177 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2265 2373 3554 10 Size: 54 1425 343 433 108 1181 3663 Fragments arranged by size: 3663 1425 1181 433 343 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 3339 3356 3777 4376 5165 5524 5683 Size: 648 2173 17 421 599 789 359 159 Cuts at: 5683 518 Size: 2042 Fragments arranged by size: 2173 2042 789 648 599 421 359 159 17 MspA1I CmG'CkG Cuts at: 1606 3247 3534 3766 3985 4000 4015 4471 4623 Size: 1641 287 232 219 15 15 456 152 Cuts at: 4623 5300 6448 6693 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1641 1148 677 456 287 245 232 219 152 15 15 NcoI C'CATG_G Cuts at: 513 1065 2488 513 Size: 552 1423 5232 Fragments arranged by size: 5232 1423 552 NdeI CA'TA_TG Cuts at: 387 2953 387 Size: 2566 4641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7207 NotI GC'GGCC_GC Cuts at: 4654 4654 Size: 7207 NspI r_CATG'y Cuts at: 1132 1229 3145 4565 6110 1132 Size: 97 1916 1420 1545 2229 Fragments arranged by size: 2229 1916 1545 1420 97 PasI CC'CwG_GG Cuts at: 1348 3757 1348 Size: 2409 4798 PciI A'CATG_T Cuts at: 3141 6106 3141 Size: 2965 4242 PflMI CCAn_nnn'nTGG Cuts at: 1108 3749 4018 1108 Size: 2641 269 4297 Fragments arranged by size: 4297 2641 269 PfoI T'CCnGG_A Cuts at: 2854 3213 3432 2854 Size: 359 219 6629 Fragments arranged by size: 6629 359 219 PmeI GTTT'AAAC Cuts at: 4511 4511 Size: 7207 PmlI CAC'GTG Cuts at: 2452 2452 Size: 7207 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3429 3461 5276 5308 6789 6821 3429 Size: 32 1815 32 1481 32 3815 Fragments arranged by size: 3815 1815 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2824 2824 Size: 7207 PshAI GACnn'nnGTC Cuts at: 1613 4373 1613 Size: 2760 4447 PsiI TTA'TAA Cuts at: 4777 4777 Size: 7207 PspOMI G'GGCC_C Cuts at: 2444 2444 Size: 7207 PstI C_TGCA'G Cuts at: 830 2866 4428 4559 830 Size: 2036 1562 131 3478 Fragments arranged by size: 3478 2036 1562 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7207 PvuII CAG'CTG Cuts at: 3534 3766 3985 4000 4015 4471 3534 Size: 232 219 15 15 456 6270 Fragments arranged by size: 6270 456 232 219 15 15 SalI G'TCGA_C Cuts at: 1936 4549 1936 Size: 2613 4594 SapI GCTCTTCn'nnn_ Cuts at: 3215 3215 Size: 7207 SbfI CC_TGCA'GG Cuts at: 4428 4559 4428 Size: 131 7076 ScaI AGT'ACT Cuts at: 5455 7167 5455 Size: 1712 5495 SfcI C'TryA_G Cuts at: 826 1045 2862 3847 3905 4424 4555 5690 6371 Size: 219 1817 985 58 519 131 1135 681 Cuts at: 6371 6562 826 Size: 191 1471 Fragments arranged by size: 1817 1471 1135 985 681 519 219 191 131 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7207 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7207 SmaI CCC'GGG Cuts at: 1872 4534 1872 Size: 2662 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2559 2634 2981 3263 4364 5311 Size: 197 926 616 75 347 282 1101 947 Cuts at: 5311 6212 6474 820 Size: 901 262 1553 Fragments arranged by size: 1553 1101 947 926 901 616 347 282 262 197 75 SnaBI TAC'GTA Cuts at: 493 2497 4998 493 Size: 2004 2501 2702 Fragments arranged by size: 2702 2501 2004 SpeI A'CTAG_T Cuts at: 152 152 Size: 7207 SphI G_CATG'C Cuts at: 1132 4565 1132 Size: 3433 3774 SspI AAT'ATT Cuts at: 5 52 5131 5 Size: 47 5079 2081 Fragments arranged by size: 5079 2081 47 StuI AGG'CCT Cuts at: 1517 3822 1517 Size: 2305 4902 StyI C'CwwG_G Cuts at: 513 1065 2488 3165 4646 513 Size: 552 1423 677 1481 3074 Fragments arranged by size: 3074 1481 1423 677 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2396 2922 3260 5234 5251 5404 5589 Size: 1255 816 526 338 1974 17 153 185 Cuts at: 5589 6034 325 Size: 445 1498 Fragments arranged by size: 1974 1498 1255 816 526 445 338 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3119 5453 7165 Size: 274 80 33 51 1443 1142 2334 1712 Cuts at: 7165 96 Size: 138 Fragments arranged by size: 2334 1712 1443 1142 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 2916 2948 3737 3769 7167 7199 2117 Size: 32 767 32 789 32 3398 32 2125 Fragments arranged by size: 3398 2125 789 767 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2045 1316 Size: 729 6478 XbaI T'CTAG_A Cuts at: 4457 4543 4457 Size: 86 7121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2366 3026 2366 Size: 660 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7207 XmaI C'CCGG_G Cuts at: 1870 4532 1870 Size: 2662 4545 XmnI GAAnn'nnTTC Cuts at: 5336 5336 Size: 7207 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2530 5015 276 Size: 53 83 186 936 996 2485 2468 Fragments arranged by size: 2485 2468 996 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI