Restriction map for
pFN21AE4376
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 1108 GAATTT [GA]^AATT[CT]
AcyI 145 GGCGCC G[GA]^CG[CT]C
AfaI 1059 GTAC GT^AC
ApaI 899 GGGCCC GGGCC^C
ApoI 1108 GAATTT [GA]^AATT[CT]
AspI 871 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 145 GGCGCC G^G[CT][GA]CC
BbeI 145 GGCGCC GGCGC^C
BbrPI 1011 CACGTG CAC^GTG
BbvCI 925 GCTGAGG CCTCAGC(-5/-2)
BsaAI 1011 CACGTG [CT]AC^GT[GA]
BsaHI 145 GGCGCC G[GA]^CG[CT]C
BsgI 25 GTGCAG GTGCAG(16/14)
BsmBI 1190 GAGACG CGTCTC(1/5)
BspDI 1136 ATCGAT AT^CGAT
BsrDI 1017 GCAATG GCAATG(2/0)
CfoI 146 GCGC GCG^C
ClaI 1136 ATCGAT AT^CGAT
DsaI 141 CCATGG C^C[GA][CT]GG
HaeII 145 GGCGCC [GA]GCGC^[CT]
HgaI 259 GACGC GACGC(5/10)
HhaI 146 GCGC GCG^C
Hin1I 145 GGCGCC G[GA]^CG[CT]C
HinP1I 146 GCGC G^CGC
HincII 1147 GTTGAC GT[CT]^[GA]AC
HindII 1147 GTTGAC GT[CT]^[GA]AC
KasI 145 GGCGCC G^GCGCC
MseI 287 TTAA T^TAA
NarI 145 GGCGCC GG^CGCC
NcoI 141 CCATGG C^CATGG
PflFI 871 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 806 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1011 CACGTG CAC^GTG
PmlI 1011 CACGTG CAC^GTG
RsaI 1059 GTAC GT^AC
SfaNI 68 GCATC GCATC(5/9)
SfoI 145 GGCGCC GGC^GCC
TaqI 1137 TCGA T^CGA
Tru9I 287 TTAA T^TAA
Tth111I 871 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1137 TCGA T^CGA
Van91I 806 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 99 CCAGCAGATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 52 GATCC GGATC(4/5)
1160 GGATC
AspHI 120 GAGCTC G[AT]GC[AT]^C
304 GAGCTC
AvaII 189 GGTCC G^G[AT]CC
222 GGTCC
BbsI 771 GAAGAC GAAGAC(2/6)
1096 GAAGAC
BpuAI 771 GAAGAC GAAGAC(2/6)
1096 GAAGAC
BseRI 163 CTCCTC GAGGAG(10/8)
928 GAGGAG
BsiHKAI 120 GAGCTC G[AT]GC[AT]^C
304 GAGCTC
BsmAI 86 GAGAC GTCTC(1/5)
1190 GAGAC
BstF5I 201 CATCC GGATG(2/0)
980 GGATG
EcoNI 234 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
996 CCTAAATCAGG
FauI 1174 CCCGC CCCGC(4/6)
1088 GCGGG
FokI 201 CATCC GGATG(9/13)
980 GGATG
HinfI 7 GACTC G^A[ACGT]TC
871 GACTC
MaeII 1012 ACGT A^CGT
1145 ACGT
PleI 7 GACTC GAGTC(4/5)
871 GACTC
PpuMI 188 GGGTCCT [GA]G^G[AT]CC[CT]
221 AGGTCCC
SacI 120 GAGCTC GAGCT^C
304 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwNI BalI BanII
BbvI BcnI BglII BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp45I Tsp509I TspRI XhoII