(Circular) (Six-base) MAPSORT of: pFN21AE4376.seq Check: 9103 from: 1 to: 5902 pFN21AE4376 5902 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 1937 Size: 1308 4594 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 2450 2519 2588 2657 2726 3175 3965 Size: 749 69 69 69 69 69 449 790 Cuts at: 3965 5349 5617 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 790 749 449 268 69 69 69 69 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 2038 2281 2350 2419 2488 2557 2626 2695 3129 Size: 243 69 69 69 69 69 69 434 Cuts at: 3129 3645 5217 2038 Size: 516 1572 2723 Fragments arranged by size: 2723 1572 516 434 243 69 69 69 69 69 69 ApaI G_GGCC'C Cuts at: 2908 2908 Size: 5902 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 3113 3211 3437 3589 5698 1493 Size: 1620 98 226 152 2109 1697 Fragments arranged by size: 2109 1697 1620 226 152 98 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 3227 1870 Size: 73 1284 4545 Fragments arranged by size: 4545 1284 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 3223 4678 618 Size: 325 217 627 363 1073 1455 1842 Fragments arranged by size: 1842 1455 1073 627 363 325 217 BanII G_rGCy'C Cuts at: 1391 2129 2313 2908 3221 1391 Size: 738 184 595 313 4072 Fragments arranged by size: 4072 738 595 313 184 BbeI G_GCGC'C Cuts at: 2154 2154 Size: 5902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2783 3108 928 Size: 971 884 325 3722 Fragments arranged by size: 3722 971 884 325 BbvCI CC'TCA_GC Cuts at: 2931 2931 Size: 5902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2337 2406 2475 2544 2613 2682 5897 2337 Size: 69 69 69 69 69 3215 2342 Fragments arranged by size: 3215 2342 69 69 69 69 69 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 2908 3963 5119 1790 Size: 1118 1055 1156 2573 Fragments arranged by size: 2573 1156 1118 1055 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3633 4591 442 Size: 416 53 83 399 24 2216 958 1753 Fragments arranged by size: 2216 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4562 1239 Size: 3323 2579 Bpu10I CC'TnA_GC Cuts at: 2931 2931 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3018 3693 493 Size: 711 1814 675 2702 Fragments arranged by size: 2702 1814 711 675 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 3709 4091 4726 Size: 53 83 186 936 618 1558 382 635 Cuts at: 4726 275 Size: 1451 Fragments arranged by size: 1558 1451 936 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2963 2993 1178 Size: 30 165 30 1560 30 4087 Fragments arranged by size: 4087 1560 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2948 1186 Size: 973 789 4140 Fragments arranged by size: 4140 973 789 BseYI C'CCAG_C Cuts at: 2038 2189 2231 2286 2355 2700 2859 2907 3050 Size: 151 42 55 69 345 159 48 143 Cuts at: 3050 5105 5601 2038 Size: 2055 496 2339 Fragments arranged by size: 2339 2055 496 345 159 151 143 69 55 48 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 1163 Size: 573 315 5014 Fragments arranged by size: 5014 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2129 2313 3963 4048 5119 2129 Size: 184 1650 85 1071 2912 Fragments arranged by size: 2912 1650 1071 184 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 BsmBI CGTCTCn'nnnn_ Cuts at: 3189 3189 Size: 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2313 2908 3221 3963 4048 5119 Size: 399 339 184 595 313 742 85 1071 Cuts at: 5119 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 595 399 339 313 184 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3257 844 Size: 2413 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3029 4397 4571 65 Size: 1183 1781 1368 174 1396 Fragments arranged by size: 1781 1396 1368 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4546 4733 1700 Size: 110 2736 187 2869 Fragments arranged by size: 2869 2736 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2056 2337 2406 2475 2544 2613 2682 3165 Size: 84 281 69 69 69 69 69 483 Cuts at: 3165 3232 3599 3984 4001 5453 5539 5551 5897 Size: 67 367 385 17 1452 86 12 346 Cuts at: 5897 1972 Size: 1977 Fragments arranged by size: 1977 1452 483 385 367 346 281 86 84 69 69 69 69 69 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2146 513 Size: 552 1081 4269 Fragments arranged by size: 4269 1081 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2376 2445 2514 2583 2652 2721 3130 3418 Size: 69 69 69 69 69 69 409 288 Cuts at: 3418 4212 4232 2307 Size: 794 20 3977 Fragments arranged by size: 3977 794 409 288 69 69 69 69 69 69 20 ClaI AT'CG_AT Cuts at: 1554 3142 1554 Size: 1588 4314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2143 2202 Size: 54 1425 50 275 18 286 27 59 Cuts at: 2202 3349 4238 5867 8 Size: 1147 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 286 275 59 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3832 1353 Size: 2479 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2127 2311 2127 Size: 184 5718 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2450 2519 2588 2657 2726 3175 Size: 393 749 69 69 69 69 69 449 Cuts at: 3175 3965 4562 5349 5617 1239 Size: 790 597 787 268 1524 Fragments arranged by size: 1524 790 787 749 597 449 393 268 69 69 69 69 69 EcoNI CCTnn'n_nnAGG Cuts at: 2243 3005 2243 Size: 762 5140 EcoO109I rG'GnC_Cy Cuts at: 2194 2227 2904 2194 Size: 33 677 5192 Fragments arranged by size: 5192 677 33 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2154 5049 1678 Size: 476 2895 2531 Fragments arranged by size: 2895 2531 476 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373 Size: 32 135 32 2971 32 42 32 2626 Fragments arranged by size: 2971 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3154 3246 3492 669 Size: 988 281 1216 92 246 3079 Fragments arranged by size: 3079 1216 988 281 246 92 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KasI G'GCGC_C Cuts at: 2150 2150 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2084 5016 5200 1305 Size: 779 2932 184 2007 Fragments arranged by size: 2932 2007 779 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2118 2145 2204 10 Size: 54 1425 343 286 27 59 3708 Fragments arranged by size: 3708 1425 343 286 59 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4219 4378 518 Size: 648 2694 359 159 2042 Fragments arranged by size: 2694 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2359 2704 2863 2953 3061 3318 3995 Size: 432 321 345 159 90 108 257 677 Cuts at: 3995 5143 5388 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 432 345 321 257 245 159 108 90 NarI GG'CG_CC Cuts at: 2151 2151 Size: 5902 NcoI C'CATG_G Cuts at: 513 1065 2146 513 Size: 552 1081 4269 Fragments arranged by size: 4269 1081 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2817 1108 Size: 1709 4193 PfoI T'CCnGG_A Cuts at: 2046 2963 2981 2046 Size: 917 18 4967 Fragments arranged by size: 4967 917 18 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PmlI CAC'GTG Cuts at: 3018 3018 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2227 2194 Size: 33 5869 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PspOMI G'GGCC_C Cuts at: 2904 2904 Size: 5902 PstI C_TGCA'G Cuts at: 830 2302 2334 2371 2403 2440 2472 2509 2541 Size: 1472 32 37 32 37 32 37 32 Cuts at: 2541 2578 2610 2647 2679 2716 2958 3254 830 Size: 37 32 37 32 37 242 296 3478 Fragments arranged by size: 3478 1472 296 242 37 37 37 37 37 37 32 32 32 32 32 32 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2038 2359 2704 2953 2038 Size: 321 345 249 4987 Fragments arranged by size: 4987 345 321 249 SacI G_AGCT'C Cuts at: 2129 2313 2129 Size: 184 5718 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2130 2298 2330 2367 2399 2436 2468 Size: 219 1085 168 32 37 32 37 32 Cuts at: 2468 2505 2537 2574 2606 2643 2675 2712 2948 Size: 37 32 37 32 37 32 37 236 Cuts at: 2948 2954 3250 4385 5066 5257 826 Size: 6 296 1135 681 191 1471 Fragments arranged by size: 1471 1135 1085 681 296 236 219 191 168 37 37 37 37 37 37 32 32 32 32 32 32 6 SfoI GGC'GCC Cuts at: 2152 2152 Size: 5902 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 2016 2024 2146 2223 2811 2830 3341 Size: 552 951 8 122 77 588 19 511 Cuts at: 3341 513 Size: 3074 Fragments arranged by size: 3074 951 588 552 511 122 77 19 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2294 2363 2432 2501 2570 2639 2708 Size: 1255 714 69 69 69 69 69 69 Cuts at: 2708 2783 3016 3064 3929 3946 4099 4284 4729 Size: 75 233 48 865 17 153 185 445 Cuts at: 4729 325 Size: 1498 Fragments arranged by size: 1498 1255 865 714 445 233 185 153 75 69 69 69 69 69 69 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3002 3034 3039 3071 5862 5894 3002 Size: 32 5 32 2791 32 3010 Fragments arranged by size: 3010 2791 32 32 32 5 Tth111I GACn'n_nGTC Cuts at: 1316 2879 1316 Size: 1563 4339 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 2111 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI